Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,034,899	T→C	pseudogene (849/1193 nt)	yedS →	putative outer membrane protein YedS, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,034,899	0	T	C	100.0%	138.8 / NA	39	pseudogene (849/1193 nt)	yedS	putative outer membrane protein YedS, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/23);  total (16/23)

TAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGTTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCATCGCTTACCTGCAATCTAAAGGTAAGGATTTAGGCGGTT  >  NC_000913/2034769‑2035034                                                                                                                                   |                                                                                                                                        tAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATg                                                                                                                                <  1:148897/140‑1 (MQ=255) tAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATg                                                                                                                                <  1:58015/140‑1 (MQ=255)  aGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGTCGAGCTTGATAATgg                                                                                                                               >  2:545020/1‑140 (MQ=255)  aGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATgg                                                                                                                               >  1:387021/1‑140 (MQ=255)  aGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATgg                                                                                                                               >  1:381231/1‑140 (MQ=255)   gCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGt                                                                                                                              >  1:67218/1‑140 (MQ=255)   gCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGt                                                                                                                              <  2:381231/140‑1 (MQ=255)   gCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGt                                                                                                                              <  2:229213/140‑1 (MQ=255)   gCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGt                                                                                                                              <  1:429224/140‑1 (MQ=255)       cAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAAt                                                                                                                          <  2:479814/140‑1 (MQ=255)       cAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAAt                                                                                                                          <  2:474722/140‑1 (MQ=255)       cAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAAt                                                                                                                          >  1:173447/1‑140 (MQ=255)        aGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATc                                                                                                                         <  2:275286/140‑1 (MQ=255)         gAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCa                                                                                                                        >  1:93972/1‑140 (MQ=255)              gCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAAt                                                                                                                                 >  2:1385/1‑126 (MQ=255)              gCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAAt                                                                                                                                 <  1:1385/126‑1 (MQ=255)                  gtggtgAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGcc                                                                                                               <  2:150238/140‑1 (MQ=255)                        aaaCCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCt                                                                                                         >  1:385921/1‑140 (MQ=255)                          aCCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTaa                                                                                                       >  1:345071/1‑140 (MQ=255)                                    cATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCaa                                                                                             <  2:67218/140‑1 (MQ=255)                                    cATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCaa                                                                                             <  2:385921/140‑1 (MQ=255)                                         aCTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACtt                                                                                        <  1:451651/140‑1 (MQ=255)                                             ttGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGaa                                                                                    >  1:515009/1‑140 (MQ=255)                                              tGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAg                                                                                   <  1:240964/140‑1 (MQ=255)                                                  gCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAgttgt                                                                               <  2:515009/140‑1 (MQ=255)                                                      aaTATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCt                                                                           <  2:387021/140‑1 (MQ=255)                                                      aaTATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCt                                                                           <  2:93972/140‑1 (MQ=255)                                                                 aataatGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGtt                                                                <  1:165225/140‑1 (MQ=255)                                                                      tgtgTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACt                                                           >  2:290764/1‑140 (MQ=255)                                                                         gtATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTg                                                        >  1:445023/1‑140 (MQ=255)                                                                           aTTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTCGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGc                                                      <  2:476026/140‑1 (MQ=255)                                                                                 cagcTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTgcgc                                                >  1:249585/1‑140 (MQ=255)                                                                                              tGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCATCGCtt                                   >  1:241661/1‑140 (MQ=255)                                                                                                 aaCTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCAt                                        >  1:444599/1‑132 (MQ=255)                                                                                                 aaCTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCAt                                        <  2:444599/132‑1 (MQ=255)                                                                                                   cTCGCAATATGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCATCGCTTACCTg                              <  2:445023/140‑1 (MQ=255)                                                                                                            tGACCCCATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCATCGCTTACCTGCAATCTaaa                     >  2:319444/1‑140 (MQ=255)                                                                                                               ccccATATGGTGGTGGCGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCATCGCTTACCTGCAATCTAAAGGt                  <  1:462928/140‑1 (MQ=255)                                                                                                                               cGAGCTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCATCGCTTACCTGCAATCTAAAGGTAAGGATTTAGGCGGtt  <  1:319444/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATGTGTATTTAGCAGCTATGTACGCTGAAACTCGCAATATGACCCCATATGGTGGTGGCGAGTTTGATAATGGTGAATCAAGATCAGCCATCGCTAACAAAACCCAAAACTTTGAAGTTGTTGCTCAGTATCAGTTCGACTTTGGCCTGCGCCCGTCCATCGCTTACCTGCAATCTAAAGGTAAGGATTTAGGCGGTT  >  NC_000913/2034769‑2035034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑