Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,048,871	A→G	K1312E (AAG→GAG)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,048,871	0	A	G	100.0%	97.9 / NA	29	K1312E (AAG→GAG)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/17);  total (12/17)

CTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCG  >  NC_000913/2048735‑2048999                                                                                                                                         |                                                                                                                                 cTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAgg                                                                                                                               >  2:472956/1‑140 (MQ=255)    gggCAGCTTCACCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTa                                                                                                                            <  1:67682/139‑1 (MQ=255)       cggCGTCACCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCg                                                                                                                         <  2:526574/138‑1 (MQ=255)       cAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCg                                                                                                                         <  2:22578/140‑1 (MQ=255)               ccaCCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTtat                                                                                                                 <  2:480194/139‑1 (MQ=255)                        caGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATAcc                                                                                                        <  1:507878/140‑1 (MQ=255)                        caGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATAcc                                                                                                        >  2:119912/1‑140 (MQ=255)                          gTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCg                                                                                                      >  2:357655/1‑140 (MQ=255)                          gTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCg                                                                                                      <  2:123971/140‑1 (MQ=255)                                     taatGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAg                                                                                           >  1:116682/1‑140 (MQ=255)                                        tGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAAt                                                                                        >  1:156019/1‑140 (MQ=255)                                           cTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAg                                                                                     >  1:304511/1‑140 (MQ=255)                                           cTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAg                                                                                     >  2:414858/1‑140 (MQ=255)                                            tttCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAgg                                                                                    >  1:259301/1‑140 (MQ=255)                                              tCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAg                                                                                  <  2:428516/140‑1 (MQ=255)                                                                   gAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAgg                                                             >  2:176536/1‑140 (MQ=255)                                                                    aaCTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGc                                                            <  2:59590/140‑1 (MQ=255)                                                                    aaCTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGc                                                            <  2:184970/140‑1 (MQ=255)                                                                    aaCTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGc                                                            <  2:304511/140‑1 (MQ=255)                                                                    aaCTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGc                                                            >  1:184970/1‑140 (MQ=255)                                                                                   gCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATgg                                             <  2:177750/140‑1 (MQ=255)                                                                                   gCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATgg                                             <  1:357655/140‑1 (MQ=255)                                                                                    cAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGt                                            <  2:156019/140‑1 (MQ=255)                                                                                    cAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGt                                            >  2:478926/1‑140 (MQ=255)                                                                                          cAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTcc                                      <  2:291574/140‑1 (MQ=255)                                                                                          cAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTcc                                      <  2:116682/140‑1 (MQ=255)                                                                                                  aTGACCATCGTCTGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCCCCTCGATAGAATCAGGAGCGAGACTGGATCCCGTTGAGGCCAGGCTGGAAAATGGTAGCGCCActgttat                              <  2:69360/140‑7 (MQ=255)                                                                                                     aCGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGcac                           <  2:38125/140‑1 (MQ=255)                                                                                                                             aacgaacAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGc   <  1:176536/140‑1 (MQ=255)                                                                                                                              acgaacAGGGTGAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCATATCAATTAATGTCAACGCTGATGCg  >  2:285727/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 CTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGGTAAGGCTACCGTCACTTATACCAATACCCGCTCCTCGATAGAATCAGGAGCGAGACCGGATACCGTTGAGGCCAGTCTGGAAAATGGTAGCTCCACGCTTAGCACATCAATTAATGTCAACGCTGATGCG  >  NC_000913/2048735‑2048999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑