Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,124,459	G→A	P21L (CCG→CTG)	cpsB ←	mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,124,459	0	G	A	100.0%	70.4 / NA	21	P21L (CCG→CTG)	cpsB	mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/12);  total (9/12)

TTGCAAATCACCACCGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGT  >  NC_000913/2124339‑2124576                                                                                                                         |                                                                                                                      ttGCAAATCACCACCGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGa                                                                                                    <  1:69276/140‑1 (MQ=255)  tGCAAATCACCACCGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAg                                                                                                   >  1:229945/1‑140 (MQ=255)  tGCAAATCACCACCGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAg                                                                                                   >  2:404771/1‑140 (MQ=255)     aaaTCACCACCGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCa                                                                                                <  1:404771/140‑1 (MQ=255)             accGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCAt                                                                                        <  2:472037/140‑1 (MQ=255)             accGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCAt                                                                                        <  2:229945/140‑1 (MQ=255)               cGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATca                                                                                      <  1:223377/140‑1 (MQ=255)               cGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATca                                                                                      <  2:270092/140‑1 (MQ=255)                            tCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATagag                                                                         >  2:288134/1‑140 (MQ=255)                                   cGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGAc                                                                  <  1:423097/140‑1 (MQ=255)                                     ttCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTg                                                                <  1:123804/140‑1 (MQ=255)                                      tCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTgc                                                               >  2:472187/1‑140 (MQ=255)                                          gcggcAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCa                                                           >  2:122248/1‑140 (MQ=255)                                           cggcAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCAt                                                          >  2:248271/1‑140 (MQ=255)                                                    gtggtTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATccc                                                 >  2:414373/1‑140 (MQ=255)                                                    gtggtTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATccc                                                 >  2:76787/1‑140 (MQ=255)                                                       gtTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGa                                              <  1:472187/140‑1 (MQ=255)                                                               caTGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTa                                                     <  1:379459/125‑1 (MQ=255)                                                               caTGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTa                                                     >  2:379459/1‑125 (MQ=255)                                                                               ttCAGGCATAAAACCTGCTTGGGATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAac                      <  1:285721/140‑1 (MQ=255)                                                                                  aGGCATAAAAACTGCTTGGGACAAAGCACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACCCCTGGATAGCGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACg                   >  2:83886/1‑140 (MQ=255)                                                                                                   gggATAAAGTACGCGGGAAAGCAGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAgcgt  <  1:414373/140‑1 (MQ=255)                                                                                                                         |                                                                                                                      TTGCAAATCACCACCGGGCTTTCGCACTCCACGCCGTTCAGGCGGCAGATGGTGGTTTGCAGCATGGTGAGATCGCCTTTCAGGCATAAAAACTGCTTGGGATAAAGTACGCGGGAAAGCGGCCATAAGCGGCTACCGGAGCCACCTGCCATCACAACTGGATAGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGT  >  NC_000913/2124339‑2124576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑