Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,455,256	T→C	T131A (ACC→GCC)	yfcV ←	putative fimbrial protein YfcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,455,256	0	T	C	100.0%	142.8 / NA	41	T131A (ACC→GCC)	yfcV	putative fimbrial protein YfcV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/26);  total (15/26)

AAATTACCCAGATCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGTACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCT  >  NC_000913/2455120‑2455387                                                                                                                                         |                                                                                                                                    aaaTTACCCAGATCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCAcc                                                                                                                                  <  1:537390/140‑1 (MQ=255)                 gcgcATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATc                                                                                                                  <  2:191900/140‑1 (MQ=255)                      tCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAg                                                                                                             >  2:357295/1‑140 (MQ=255)                      tCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAg                                                                                                             >  1:424853/1‑140 (MQ=255)                               ccGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGc                                                                                                                <  1:405712/128‑1 (MQ=255)                               ccGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGc                                                                                                                >  2:405712/1‑128 (MQ=255)                                  aCCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGtt                                                                                                 >  2:352821/1‑140 (MQ=255)                                  aCCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGtt                                                                                                 >  1:16698/1‑140 (MQ=255)                                        cagGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGc                                                                                           <  1:336995/140‑1 (MQ=255)                                                   gTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACcggtat                                                                                <  2:424853/140‑1 (MQ=255)                                                         agTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGcc                                                                                          >  2:526315/1‑124 (MQ=255)                                                         agTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGcc                                                                                          <  1:526315/124‑1 (MQ=255)                                                           tCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTg                                                                        >  2:523395/1‑140 (MQ=255)                                                                 tCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATg                                                                  <  1:352821/140‑1 (MQ=255)                                                                   gCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGgt                                                                <  1:357295/140‑1 (MQ=255)                                                                   gCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGgt                                                                >  2:50573/1‑140 (MQ=255)                                                                                       aGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTg                                            >  1:229033/1‑140 (MQ=255)                                                                                       aGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTg                                            <  1:523395/140‑1 (MQ=255)                                                                                        gTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGc                                           >  1:488335/1‑140 (MQ=255)                                                                                           tCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCaga                                        <  2:70866/140‑1 (MQ=255)                                                                                                cACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGaaa                                   <  2:109317/140‑1 (MQ=255)                                                                                                cACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGaaa                                   <  2:16698/140‑1 (MQ=255)                                                                                                cACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGaaa                                   <  1:512383/140‑1 (MQ=255)                                                                                                cACGATTTTCTGGTAAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGaaa                                   <  2:536280/140‑1 (MQ=255)                                                                                                 aCGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAAc                                  >  1:478834/1‑140 (MQ=255)                                                                                                     ttttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTa                              <  2:229033/140‑1 (MQ=255)                                                                                                     ttttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTa                              <  1:405583/140‑1 (MQ=255)                                                                                                      tttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTAc                             <  2:488335/140‑1 (MQ=255)                                                                                                       ttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTAcc                            <  1:50573/140‑1 (MQ=255)                                                                                                       ttCTGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTAcc                            <  1:491082/140‑1 (MQ=255)                                                                                                          tGGTCAATACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGt                         <  2:242191/140‑1 (MQ=255)                                                                                                                 tACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTAcc                            <  2:332479/130‑1 (MQ=255)                                                                                                                 tACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTAcc                            >  1:332479/1‑130 (MQ=255)                                                                                                                 tACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTa                  <  2:260158/140‑1 (MQ=255)                                                                                                                 tACCCATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTa                  <  1:203440/140‑1 (MQ=255)                                                                                                                     cATGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTgg              <  1:10444/140‑1 (MQ=255)                                                                                                                      aTGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGt             >  2:62282/1‑140 (MQ=255)                                                                                                                       tGCCGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTc            <  2:231949/140‑1 (MQ=255)                                                                                                                         ccGCTTTTGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCAt          >  2:158236/1‑140 (MQ=255)                                                                                                                             ttttGTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGtt      >  1:399276/1‑140 (MQ=255)                                                                                                                                 gTAAGAGGCACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCt  <  2:392134/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    AAATTACCCAGATCTGGCGCATCAGCTGCGCCGACCAGCCAGGCTTTAAAGTTCAGAGTCTGCTTCGCTTTGCCTTTATTAGTGGCAGTATCTTTCACGATTTTCTGGTCAATACCCATGCCGCTTTTGTAAGAGGTACCCAGAGAGTCACCGATCGCCAGGCTAACATTGTTAAATGCCGCACCGGTATCGGTATTGAAAATGGTGTAATAATTGCCGCTATTTGCAGAAGAAACGGTACCAGTAAAGGTAGTGGTCATTGTTTCCT  >  NC_000913/2455120‑2455387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑