Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,459,911	T→C	T187A (ACC→GCC)	fadI ←	3‑ketoacyl‑CoA thiolase FadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,459,911	0	T	C	100.0%	92.6 / NA	29	T187A (ACC→GCC)	fadI	3‑ketoacyl‑CoA thiolase FadI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

GGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGAC  >  NC_000913/2459775‑2460031                                                                                                                                         |                                                                                                                         gggATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTc                                                                                                                       >  1:275064/1‑140 (MQ=255)  ggATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCg                                                                                                                      >  2:19132/1‑140 (MQ=255)               catcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGc                                                                                                         <  1:293177/140‑1 (MQ=255)                       cttcttTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGaa                                                                                                 <  2:519637/140‑1 (MQ=255)                              gAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTg                                                                                          <  2:275064/140‑1 (MQ=255)                                     ccGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGc                                                                                   <  1:227761/140‑1 (MQ=255)                                           gACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAggtgg                                                                             >  2:42647/1‑140 (MQ=255)                                           gACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAggtgg                                                                             >  2:319972/1‑140 (MQ=255)                                             ccATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTa                                                                           <  2:445849/140‑1 (MQ=255)                                                 gCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACggg                                                                       >  1:118424/1‑140 (MQ=255)                                                      aGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTa                                                                  >  1:455614/1‑140 (MQ=255)                                                      aGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTa                                                                  >  1:504008/1‑140 (MQ=255)                                                      aGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTa                                                                  >  2:242450/1‑140 (MQ=255)                                                         ggCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGt                                                               <  2:455614/140‑1 (MQ=255)                                                                          ggTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCga                                              <  1:319972/140‑1 (MQ=255)                                                                          ggTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCga                                              <  1:42647/140‑1 (MQ=255)                                                                           gTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCACCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCgag                                             <  2:3070/140‑1 (MQ=255)                                                                                    tGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAgg                                                 <  1:364067/127‑1 (MQ=255)                                                                                    tGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAgg                                                 >  2:364067/1‑127 (MQ=255)                                                                                           tGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCg                             <  1:19132/140‑1 (MQ=255)                                                                                                ttCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCACAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGc                        >  1:426947/1‑140 (MQ=255)                                                                                                     gggTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATg                   >  2:423497/1‑140 (MQ=255)                                                                                                      ggTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATgg                  <  2:118424/140‑1 (MQ=255)                                                                                                         gATGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTAc               <  1:9217/140‑1 (MQ=255)                                                                                                          aTGCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACg              >  2:391618/1‑140 (MQ=255)                                                                                                            gCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAg            <  2:504008/140‑1 (MQ=255)                                                                                                            gCCGTAGGTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAg            >  1:112773/1‑140 (MQ=255)                                                                                                                  ggTTTTCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTttgt      <  1:391618/140‑1 (MQ=255)                                                                                                                      ttCGCCATTTGCTCTGCGGCGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCACAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGAc  <  2:426947/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                         GGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGAC  >  NC_000913/2459775‑2460031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑