Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,604,871	T→C	F21L (TTT→CTT)	hyfD →	hydrogenase 4 component D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,604,871	0	T	C	100.0%	122.7 / NA	36	F21L (TTT→CTT)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

ACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGTCTTCTGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGG  >  NC_000913/2604735‑2605004                                                                                                                                         |                                                                                                                                      aCCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGTCTTCTGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGcttt                                                                                                                                    <  1:317421/140‑1 (MQ=255)           gcttCAGCCTTGCTGCGCTTGCATGGGTCTTCTGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACg                                                                                                                          >  1:493985/1‑140 (MQ=255)                   cTTGCTGCGCTTGCATGGGTCTTCTGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGgccg                                                                                                                  <  1:447810/140‑1 (MQ=255)                          cgcTTGCATGGGTCTTCTGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATg                                                                                                           <  2:232487/140‑1 (MQ=255)                                cATGGGTCTTCTGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGt                                                                                                     >  2:110985/1‑140 (MQ=255)                                      tcttctGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGtt                                                                                               >  2:47665/1‑140 (MQ=255)                                         tctGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTcgc                                                                                            >  1:165542/1‑140 (MQ=255)                                              ttAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCgcgc                                                                                       >  1:449531/1‑140 (MQ=255)                                                aaCCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTg                                                                                     >  1:158052/1‑140 (MQ=255)                                                aaCCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTg                                                                                     >  1:527733/1‑140 (MQ=255)                                                    ggTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCa                                                                                 <  1:110985/140‑1 (MQ=255)                                                     gTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTcg                                                                                             <  1:394292/127‑1 (MQ=255)                                                     gTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTcg                                                                                             >  2:394292/1‑127 (MQ=255)                                                     gTCTGTAAGGAGCACTGACGAAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCACCGCGCTGACCAc                                                                                <  1:401561/140‑1 (MQ=255)                                                       cTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGc                                                                              >  2:183490/1‑140 (MQ=255)                                                               ggCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCAtg                                                                      <  2:257890/139‑1 (MQ=255)                                                               aGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCAtg                                                                      <  1:205112/140‑1 (MQ=255)                                                                gCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCAtgt                                                                     <  2:449531/140‑1 (MQ=255)                                                                 cACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCAtgtt                                                                    >  2:152830/1‑140 (MQ=255)                                                                  aCTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCAtgttg                                                                   >  2:254947/1‑140 (MQ=255)                                                                  aCTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCAtgttg                                                                   >  2:111806/1‑140 (MQ=255)                                                                  aCTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCAtgtcg                                                                   >  1:383452/1‑138 (MQ=255)                                                                    tGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTc                                                                 >  1:307569/1‑140 (MQ=255)                                                                      aCGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCAc                                                               <  2:527733/140‑1 (MQ=255)                                                                       cGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACt                                                              <  2:307569/140‑1 (MQ=255)                                                                        ggAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTg                                                             >  1:155599/1‑140 (MQ=255)                                                                                       ttGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTAt                                              <  1:254947/140‑1 (MQ=255)                                                                                        tGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATc                                             >  1:359904/1‑140 (MQ=255)                                                                                          ctctGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAg                                           <  1:227904/140‑1 (MQ=255)                                                                                               acgacgTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGc                                         <  1:274156/137‑1 (MQ=255)                                                                                               acgacgTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGc                                         >  2:274156/1‑137 (MQ=255)                                                                                                   cgTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATaaa                                  <  2:229892/140‑1 (MQ=255)                                                                                                         tgctgcCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGcc                            >  1:471998/1‑140 (MQ=255)                                                                                                         tgctgcCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGcc                            >  2:290791/1‑140 (MQ=255)                                                                                                             gcCTTTTATCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCa                        >  2:223409/1‑140 (MQ=255)                                                                                                                     tCGGCGCACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacgttgacg                <  1:183490/140‑1 (MQ=255)                                                                                                                          gcACTGGTCGTTTCGCTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGt           <  1:111806/140‑1 (MQ=255)                                                                                                                                   gtttcgcttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTggg  <  2:471998/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      ACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGTCTTCTGGTTAACCGGTCTGTAAGGAGCACTGACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGG  >  NC_000913/2604735‑2605004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑