Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,634,366	T→C	G45G (GGT→GGC)	xseA →	exodeoxyribonuclease VII subunit XseA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,634,366	0	T	C	100.0%	115.6 / NA	33	G45G (GGT→GGC)	xseA	exodeoxyribonuclease VII subunit XseA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

ACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTA  >  NC_000913/2634230‑2634490                                                                                                                                         |                                                                                                                             acaTGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCAc                                                                                                                           <  1:262855/140‑1 (MQ=255)  caTGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCAc                                                                                                                           >  2:461124/1‑139 (MQ=255)  caTGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCAc                                                                                                                           <  1:461124/139‑1 (MQ=255)   aTGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTg                                                                                                                         <  2:454324/140‑1 (MQ=255)           ttCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTa                                                                                                                 <  2:491299/140‑1 (MQ=255)             ctcAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTaca                                                                                                               >  1:72669/1‑140 (MQ=255)                aaTCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTc                                                                                                            >  1:191569/1‑140 (MQ=255)                       tGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTa                                                                                                                 >  1:378778/1‑128 (MQ=255)                       tGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTa                                                                                                                 <  2:378778/128‑1 (MQ=255)                       tGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAgacg                                                                                                     >  2:482961/1‑140 (MQ=255)                          aaTTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGaca                                                                                                  >  2:238891/1‑140 (MQ=255)                                            ccTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTgcg                                                                                <  1:208598/140‑1 (MQ=255)                                              tGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACACGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGcgcg                                                                              <  2:504916/140‑1 (MQ=255)                                                aaTCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGAt                                                                            >  2:521729/1‑140 (MQ=255)                                                 aTCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATg                                                                           <  2:121785/140‑1 (MQ=255)                                                                    gctTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAAccgcc                                                        <  2:281690/140‑1 (MQ=255)                                                                    gctTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAAccgcc                                                        >  1:281690/1‑140 (MQ=255)                                                                         aGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTg                                                   >  1:198026/1‑140 (MQ=255)                                                                            aTGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGAcc                                                >  1:186487/1‑140 (MQ=255)                                                                              gagaTGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCtt                                              <  2:191569/140‑1 (MQ=255)                                                                               agaTGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTc                                             >  2:329691/1‑140 (MQ=255)                                                                                                  cagcagCGAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGcaac                          <  2:500095/140‑1 (MQ=255)                                                                                                    gcggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGcaacaa                        >  2:20739/1‑140 (MQ=255)                                                                                                    gcggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGcaacaa                        >  1:491725/1‑140 (MQ=255)                                                                                                    gcggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGcaacaa                        >  2:302827/1‑140 (MQ=255)                                                                                                     cggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAg                       >  1:174433/1‑140 (MQ=255)                                                                                                             tttCTAATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGtt               <  2:290981/140‑1 (MQ=255)                                                                                                              ttCTTATTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTc              <  2:500653/140‑1 (MQ=255)                                                                                                                  aaTTTCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCgcgc          <  1:20739/140‑1 (MQ=255)                                                                                                                    tttCACGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCa        <  1:482961/140‑1 (MQ=255)                                                                                                                         cGCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATAtt   >  1:376038/1‑140 (MQ=255)                                                                                                                          gCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTa  <  1:329691/140‑1 (MQ=255)                                                                                                                          gCAACCAGCTTCCGGCCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTa  <  1:521729/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             ACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCTGAATCAAACGGTTCGTCTGCTGCTTGAGCATGAGATGGGACAGGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTA  >  NC_000913/2634230‑2634490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑