Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,637,605	T→C	N350D (AAC→GAC)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,637,605	0	T	C	100.0%	154.6 / NA	44	N350D (AAC→GAC)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

GACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTTAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCGTAACGCCGCCATCAATGGTCA  >  NC_000913/2637469‑2637730                                                                                                                                         |                                                                                                                              gaCGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAAt                                                                                                                            <  1:520024/140‑1 (MQ=255)  aCGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAAGCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATc                                                                                                                           <  2:183569/140‑1 (MQ=255)  aCGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATc                                                                                                                           <  2:362635/140‑1 (MQ=255)     aTTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAg                                                                                                                        <  1:302511/140‑1 (MQ=255)          cGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAg                                                                                                                   <  1:523179/140‑1 (MQ=255)            tgtAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGAt                                                                                                                 >  1:146712/1‑140 (MQ=255)                        cacGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGt                                                                                                     >  2:68172/1‑140 (MQ=255)                           ggTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCAc                                                                                                  >  2:270590/1‑140 (MQ=255)                             ttCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCg                                                                                                >  1:107664/1‑140 (MQ=255)                             ttCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCg                                                                                                >  2:73963/1‑140 (MQ=255)                             ttCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCg                                                                                                >  2:33977/1‑140 (MQ=255)                             ttCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCg                                                                                                >  2:29551/1‑140 (MQ=255)                             ttCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCg                                                                                                >  2:221142/1‑140 (MQ=255)                              tCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGa                                                                                               >  2:483150/1‑140 (MQ=255)                              tCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGa                                                                                               >  2:173832/1‑140 (MQ=255)                                       tGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGt                                                                                      <  1:122537/140‑1 (MQ=255)                                       tGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGt                                                                                      <  2:146712/140‑1 (MQ=255)                                       tGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGt                                                                                      <  1:270590/140‑1 (MQ=255)                                          cTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGc                                                                                   <  1:483150/140‑1 (MQ=255)                                          cTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGc                                                                                   <  2:33387/140‑1 (MQ=255)                                            ggATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCa                                                                                 <  1:68172/140‑1 (MQ=255)                                            ggATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCa                                                                                 >  2:249128/1‑140 (MQ=255)                                                 agcagTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATtata                                                                            <  2:413436/140‑1 (MQ=255)                                                 agcagTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATtata                                                                            <  2:253417/140‑1 (MQ=255)                                                      tttGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCa                                                                       <  1:73963/140‑1 (MQ=255)                                                        tGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCAcc                                                                     >  2:269608/1‑140 (MQ=255)                                                        tGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCAcc                                                                     >  2:206158/1‑140 (MQ=255)                                                              cAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGaa                                                               <  1:435198/140‑1 (MQ=255)                                                                     aaCCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTcagca                                                        <  2:107664/140‑1 (MQ=255)                                                                       ccGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAgg                                                      <  1:249128/140‑1 (MQ=255)                                                                          gTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGa                                                   <  1:368994/140‑1 (MQ=255)                                                                                tCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATgcagc                                             >  2:523723/1‑140 (MQ=255)                                                                                    gAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGTCGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGAt                                         >  2:371981/1‑140 (MQ=255)                                                                                       aCCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGc                                      <  1:206158/140‑1 (MQ=255)                                                                                          ggAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCttt                                   >  1:33642/1‑140 (MQ=255)                                                                                          ggAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCttt                                   >  2:152996/1‑140 (MQ=255)                                                                                           gAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTtg                                  <  2:171506/140‑1 (MQ=255)                                                                                                      tttttGCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCg                       >  1:105561/1‑140 (MQ=255)                                                                                                           gCTGGGCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCGTAAcg                  <  1:523723/140‑1 (MQ=255)                                                                                                               ggCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCGTAAcgccgc              >  1:31464/1‑140 (MQ=255)                                                                                                                gCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCGTAAcg                  <  1:294852/135‑1 (MQ=255)                                                                                                                gCAACGAAACGACCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCGTAAcg                  >  2:294852/1‑135 (MQ=255)                                                                                                                         cgaCCATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCGTAACGCCGCCATCAATGGt    >  2:210383/1‑140 (MQ=255)                                                                                                                           cccATCTTCGACGTCAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGATAAGTCAGGAGGCGATGCAGCAGATCGCTTTGTGTGCACAGCGTAACGCCGCCATCAATGGTCa  <  2:347961/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                              GACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAACGAAACGACCATCTTCGACGTTAATCCAGTGCAGATAACCTTCACTGTCACCGACCACCAGGTTGCCATTATACAGCACCGGAGAAGTCAGCAGGCGATGCAGCAGATCGCTTTGTGTCCACAGCGTAACGCCGCCATCAATGGTCA  >  NC_000913/2637469‑2637730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑