Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,670,206	T→C	L392P (CTG→CCG)	hcaE →	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,670,206	0	T	C	100.0%	82.3 / NA	24	L392P (CTG→CCG)	hcaE	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/15);  total (9/15)

CGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACTGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCT  >  NC_000913/2670069‑2670340                                                                                                                                          |                                                                                                                                       cGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACcgt                                                                                                                                      <  2:153152/140‑1 (MQ=255)        gTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGa                                                                                                                               >  1:66805/1‑140 (MQ=255)           aaaGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAAt                                                                                                                            >  1:298842/1‑140 (MQ=255)                 gCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCt                                                                                                                      <  2:66805/140‑1 (MQ=255)                 gCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCt                                                                                                                      <  2:304560/140‑1 (MQ=255)                  cTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCtt                                                                                                                     >  1:197282/1‑140 (MQ=255)                               cgcCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAgc                                                                                                        >  1:301849/1‑140 (MQ=255)                                            tttGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACgg                                                                                           <  2:197282/140‑1 (MQ=255)                                            tttGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACgg                                                                                           <  1:253620/140‑1 (MQ=255)                                             ttGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGc                                                                                          <  1:137897/140‑1 (MQ=255)                                                gTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCAtt                                                                                       <  1:86682/140‑1 (MQ=255)                                                    tgctgGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTg                                                                                   <  2:298842/140‑1 (MQ=255)                                                     gctgGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTgg                                                                                  <  1:58275/140‑1 (MQ=255)                                                     gctgGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTgg                                                                                  >  2:455430/1‑140 (MQ=255)                                                      ctgGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGc                                                                                 <  2:364895/140‑1 (MQ=255)                                                          ttttCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAAGAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTa                                                                             <  2:221775/140‑1 (MQ=255)                                                           tttCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTac                                                                            <  1:426636/140‑1 (MQ=255)                                                               tcGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTactaac                                                                        <  1:488589/140‑1 (MQ=255)                                                                                 agaACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACt                                                      >  2:481462/1‑140 (MQ=255)                                                                                   aaCTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGc                                                    <  1:481462/140‑1 (MQ=255)                                                                                             aaaTCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGa                                          >  1:452897/1‑140 (MQ=255)                                                                                                  cAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTa                                     >  1:530627/1‑140 (MQ=255)                                                                                                                aGGCCACCGCGCCCGCAACAGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCg                       <  2:301849/140‑1 (MQ=255)                                                                                                                                   aGCAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAg    <  2:530627/140‑1 (MQ=255)                                                                                                                                     cAAACCGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCt  >  1:363049/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGGTGTGAAATCCAGAAATTGCTTAAAGGCCACCGCGCCCGCAACAGCAAACTGTGTCTGGAAATGGGGCTTGGTCAGGAAAAGCGCCGCGACGACGGCATTCCTGGCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCT  >  NC_000913/2670069‑2670340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑