Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,680,354	C→T	A798T (GCC→ACC)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,680,354	0	C	T	100.0%	89.2 / NA	27	A798T (GCC→ACC)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

TTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAG  >  NC_000913/2680219‑2680490                                                                                                                                        |                                                                                                                                         tttCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGc                                                                                                                                      >  1:338457/1‑140 (MQ=255)           attTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCgt                                                                                                                            <  2:488231/140‑1 (MQ=255)                      gcgcCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTc                                                                                                                 >  1:109747/1‑140 (MQ=255)                      gcgcCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTc                                                                                                                 <  1:227137/140‑1 (MQ=255)                      gcgcCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTc                                                                                                                 <  2:336305/140‑1 (MQ=255)                      gcgcCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTc                                                                                                                 <  1:5488/140‑1 (MQ=255)                              cGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATg                                                                                                         <  2:74635/140‑1 (MQ=255)                                  tctcCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATc                                                                                                     >  2:231284/1‑140 (MQ=255)                                           tgGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCcg                                                                                            >  2:45101/1‑140 (MQ=255)                                              cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCgcgc                                                                                         >  1:113142/1‑140 (MQ=255)                                                 cACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAg                                                                                      >  2:346709/1‑140 (MQ=255)                                                   cttaacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGcc                                                                                    >  2:150676/1‑140 (MQ=255)                                                           aTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCaa                                                                            >  2:208351/1‑140 (MQ=255)                                                             ccAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAAc                                                                          >  2:348492/1‑140 (MQ=255)                                                                     tCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGa                                                                  <  1:208351/140‑1 (MQ=255)                                                                      cccGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGAc                                                                 >  2:497/1‑140 (MQ=255)                                                                          ttCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACAtct                                                             >  2:309113/1‑140 (MQ=255)                                                                                    aGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACg                                                   <  2:499741/140‑1 (MQ=255)                                                                                                tGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGc                                       <  1:231284/140‑1 (MQ=255)                                                                                                     cgcAAGCTGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATgg                                  <  2:109747/140‑1 (MQ=255)                                                                                                            tGATAAGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTttgtt                           <  1:45101/140‑1 (MQ=255)                                                                                                                 aGCATTATCAAGATAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGc                      <  1:497/140‑1 (MQ=255)                                                                                                                             aTAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTtgttgt          >  2:394522/1‑140 (MQ=255)                                                                                                                             aTAACGCGCGGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTtgttgt          >  2:495417/1‑140 (MQ=255)                                                                                                                                 cgcgcgGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTgtagta      >  1:531965/1‑140 (MQ=255)                                                                                                                                 cgcgcgGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGGTGTAGCTACGTTTGTTgtagta      >  1:384562/1‑140 (MQ=255)                                                                                                                                     cgGTCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGaag  <  2:338457/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTTTGTTGTAGCTACGTTTGTTGTAGTAGAAG  >  NC_000913/2680219‑2680490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑