Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,212	G→A	A173V (GCG→GTG)	pdxJ ←	pyridoxine 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,212	0	G	A	100.0%	111.9 / NA	34	A173V (GCG→GTG)	pdxJ	pyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/22);  total (12/22)

TTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTG  >  NC_000913/2701097‑2701345                                                                                                                    |                                                                                                                                      ttCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGt                                                                                                               >  1:176262/1‑140 (MQ=255)      gCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGtt                                                                                                          >  2:35929/1‑140 (MQ=255)      gCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGtt                                                                                                          <  1:71270/140‑1 (MQ=255)           tcAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGc                                                                                                     <  2:114275/140‑1 (MQ=255)                    gcggcAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATa                                                                                            >  1:279720/1‑140 (MQ=255)                    gcggcAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATa                                                                                            >  1:401857/1‑140 (MQ=255)                    gcggcAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATa                                                                                            >  2:296388/1‑140 (MQ=255)                       gcAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCa                                                                                         >  1:196696/1‑140 (MQ=255)                        cAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCaa                                                                                        <  1:245326/140‑1 (MQ=255)                         aaTGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAAc                                                                                       <  2:93567/140‑1 (MQ=255)                         aaTGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAAc                                                                                       <  2:310177/140‑1 (MQ=255)                         aaTGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAAc                                                                                       <  2:176262/140‑1 (MQ=255)                                       tgtgATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCg                                                                         >  2:458949/1‑140 (MQ=255)                                          gATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATa                                                                      <  2:18697/140‑1 (MQ=255)                                              ggTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACg                                                                  <  2:190606/140‑1 (MQ=255)                                              ggTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACg                                                                  <  2:508952/140‑1 (MQ=255)                                                   gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                             <  2:316242/140‑1 (MQ=255)                                                   gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTgcg                                                             >  1:433946/1‑140 (MQ=255)                                                   gACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGCCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGCGGATCTCGATAAACgccgcg                                                             >  2:239437/1‑135 (MQ=255)                                                                   aaCTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAg                                             >  1:277135/1‑140 (MQ=255)                                                                     cTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCt                                           <  2:131206/140‑1 (MQ=255)                                                                          aGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGAt                                      <  2:238545/140‑1 (MQ=255)                                                                            aCCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCg                                                <  2:411835/128‑1 (MQ=255)                                                                            aCCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCg                                                >  1:411835/1‑128 (MQ=255)                                                                                        ggCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCgg                        <  2:124177/140‑1 (MQ=255)                                                                                        ggCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCgg                        <  2:449661/140‑1 (MQ=255)                                                                                        ggCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCgg                        <  2:196696/140‑1 (MQ=255)                                                                                        ggCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCgg                        <  1:458949/140‑1 (MQ=255)                                                                                        ggCAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCAGCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCgg                        <  2:433946/140‑1 (MQ=255)                                                                                        ggCAAAAGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCgg                        <  1:153513/140‑1 (MQ=255)                                                                                          cAAAGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCg                      <  2:277135/140‑1 (MQ=255)                                                                                            aaGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTTGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGAt                                      <  2:68574/122‑1 (MQ=255)                                                                                            aaGGTCGCGGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTTGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGAt                                      >  1:68574/1‑122 (MQ=255)                                                                                                 cgcgGCTTTGGCGATACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATaa               >  2:263982/1‑140 (MQ=255)                                                                                                              aTACGCACCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTg  <  2:279720/140‑1 (MQ=255)                                                                                                                    |                                                                                                                                      TTCATGCATCTCAGGGATGGCGGCAATGGCTTTCACGTTGTGATAGGTCAGACCGTGTCCGGCGTTAACTTTCAGACCGAGGCTTGCGGCAAAGGTCGCGGCTTTGGCGATACGCGCCAGCTCTTGCGCCTGTTCGGCGTCAGTTTTGGCATCAGCATAGCAACCGGTGTGGATCTCGATAAACGGTGCGCCAACCTCTGCCGCAGCTTTGATCTGCTCTTCATCGGCGTCAATAAACAGAGAAACCTG  >  NC_000913/2701097‑2701345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑