Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,800,337	T→C	R64R (CGT→CGC)	ygaM →	DUF883 domain‑containing protein YgaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,800,337	0	T	C	100.0%	125.2 / NA	36	R64R (CGT→CGC)	ygaM	DUF883 domain‑containing protein YgaM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

TGTCAATCAATTAGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCG  >  NC_000913/2800205‑2800465                                                                                                                                     |                                                                                                                                 tgTCAATCAATTAGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTcgcg                                                                                                                           >  2:430341/1‑140 (MQ=255) tgTCAATCAATTAGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTcgcg                                                                                                                           >  2:394865/1‑140 (MQ=255)             aGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCcg                                                                                                               >  2:364074/1‑140 (MQ=255)               cTGGCAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCcgcg                                                                                                             >  1:370987/1‑140 (MQ=255)                     gCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGAt                                                                                                       >  2:241002/1‑140 (MQ=255)                         cGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCg                                                                                                   >  1:23531/1‑140 (MQ=255)                                 tATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAGACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgc                                                                                           >  2:419741/1‑140 (MQ=255)                                 tATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgc                                                                                           >  2:537951/1‑140 (MQ=255)                                   ttGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgcgc                                                                                         <  1:151603/140‑1 (MQ=255)                                   ttGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTgcgc                                                                                         <  1:364074/140‑1 (MQ=255)                                      aaGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGa                                                                                      >  1:528953/1‑140 (MQ=255)                                      aaGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGa                                                                                      >  1:238847/1‑140 (MQ=255)                                         tCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTc                                                                                   >  2:439353/1‑140 (MQ=255)                                           cTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCtt                                                                                 >  1:289186/1‑140 (MQ=255)                                           cTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCtt                                                                                 >  1:526244/1‑140 (MQ=255)                                                gCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGtt                                                                            <  1:394865/140‑1 (MQ=255)                                                   gCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGt                                                                         >  1:169375/1‑140 (MQ=255)                                                       cGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGaaa                                                                     <  2:23531/140‑1 (MQ=255)                                                         ccAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGa                                                                   <  1:73468/140‑1 (MQ=255)                                                           aaaGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGAcc                                                                 <  1:32865/140‑1 (MQ=255)                                                           aaaGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGAcc                                                                 <  1:430341/140‑1 (MQ=255)                                                            aaGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGAccc                                                                <  1:439353/140‑1 (MQ=255)                                                              ggggAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTg                                                              <  2:169375/140‑1 (MQ=255)                                                                 gAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGgtg                                                           <  1:160736/140‑1 (MQ=255)                                                                                          cTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCggta                                  >  1:343564/1‑140 (MQ=255)                                                                                             aGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCggtaggt                               >  2:55184/1‑140 (MQ=255)                                                                                               gCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTAt                             >  1:471139/1‑140 (MQ=255)                                                                                                          agagaCCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGgcg                  <  1:419741/140‑1 (MQ=255)                                                                                                           gaaaCCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGgcgc                 <  2:370987/140‑1 (MQ=255)                                                                                                            aaaCCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCa                <  1:131430/140‑1 (MQ=255)                                                                                                             aaCCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCAc               >  2:499822/1‑140 (MQ=255)                                                                                                             aaCCAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCAc               <  2:528953/140‑1 (MQ=255)                                                                                                               ccAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGgcgc                 <  1:32177/136‑1 (MQ=255)                                                                                                               ccAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGgcgc                 >  2:32177/1‑136 (MQ=255)                                                                                                                cAGGGCGCGAATGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGt            <  1:537951/140‑1 (MQ=255)                                                                                                                          aTGCATGGTCGCACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATgcg  >  2:150680/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                 TGTCAATCAATTAGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCG  >  NC_000913/2800205‑2800465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑