Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,848,921	G→A	N107N (AAC→AAT)	hycC ←	formate hydrogenlyase subunit HycC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,848,921	0	G	A	100.0%	103.6 / NA	30	N107N (AAC→AAT)	hycC	formate hydrogenlyase subunit HycC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/12);  total (18/12)

TCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCGTTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTACCAGCGACAGCGCA  >  NC_000913/2848793‑2849056                                                                                                                                 |                                                                                                                                        tctTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCAc                                                                                                                              >  2:240559/1‑140 (MQ=255) tctTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCAc                                                                                                                              <  2:126873/140‑1 (MQ=255)       ctgttgctgGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTgcgc                                                                                                                        <  1:240559/140‑1 (MQ=255)            gctgGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGc                                                                                                                   <  1:314964/140‑1 (MQ=255)                     gAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGt                                                                                                          >  2:471553/1‑140 (MQ=255)                       acacCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCa                                                                                                        <  1:473971/140‑1 (MQ=255)                        cacCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCaa                                                                                                       >  1:305229/1‑140 (MQ=255)                          ccGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAAtg                                                                                                     >  2:74083/1‑140 (MQ=255)                                    ggCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGc                                                                                           >  1:147557/1‑140 (MQ=255)                                          gATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACaa                                                                                     <  1:336615/140‑1 (MQ=255)                                              tCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAg                                                                                 >  1:390839/1‑140 (MQ=255)                                                       aCCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAg                                                                        >  2:35952/1‑140 (MQ=255)                                                         cACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGAc                                                                      <  2:147557/140‑1 (MQ=255)                                                           cGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCg                                                                    >  1:528951/1‑140 (MQ=255)                                                                aTGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGt                                                               >  2:48626/1‑140 (MQ=255)                                                                 tGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTa                                                              >  2:225911/1‑140 (MQ=255)                                                                          tGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCaa                                                     >  2:71327/1‑140 (MQ=255)                                                                           gCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCaaa                                                    >  1:344198/1‑140 (MQ=255)                                                                             tGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATc                                                  <  1:471553/140‑1 (MQ=255)                                                                                cAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATcgcg                                               >  2:96439/1‑140 (MQ=255)                                                                                    gACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTaa                                           <  1:444108/140‑1 (MQ=255)                                                                                         cgcAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGga                                      <  1:74083/140‑1 (MQ=255)                                                                                           cAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGgaga                                    <  2:344198/140‑1 (MQ=255)                                                                                                   ggcAGCCATCAACATATTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGca                            <  2:305229/140‑1 (MQ=255)                                                                                                                 tatTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTAcc              >  1:86644/1‑140 (MQ=255)                                                                                                                  atTGATCTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGTCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTACCa             >  2:347171/1‑140 (MQ=255)                                                                                                                        cTGCAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTACCAGCGACa       >  1:491915/1‑140 (MQ=255)                                                                                                                           cAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTACCAGCGACAgcg    >  2:230796/1‑140 (MQ=255)                                                                                                                           cAAGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTACCAGCGACAgcg    >  1:428483/1‑140 (MQ=255)                                                                                                                             aGCCATTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTACCAGCGACAGCGCa  <  1:96439/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        TCTTTGCTGTTGCTGGTGAGGAACACCGCGCACAGGGCCATGATTTCGGCCATTACCACGAACATGCCGAGGTTGCTGGCAATGACGGCGCAGACGGCGGCAGCCATCAACATATTGATCTGCAAGCCGTTGCACTTCACCTGCGCGTGGCGATGCCAGTCAATGTTGTAGAGGCTGACAAACAGACCGCACAGACCGAGCGTAATCAGCCAAATCGCGTTAAGCGGAGAGATTTGCACATCGTAGCTTACCAGCGACAGCGCA  >  NC_000913/2848793‑2849056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑