Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,863,781	A→G	K63K (AAA→AAG)	ygbL →	putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,863,781	0	A	G	100.0%	89.5 / NA	26	K63K (AAA→AAG)	ygbL	putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

TCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTT  >  NC_000913/2863653‑2863914                                                                                                                                 |                                                                                                                                      tCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGa                                                                                                                            >  2:346178/1‑140 (MQ=255)      cTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCg                                                                                                                       >  2:90789/1‑140 (MQ=255)        ttCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGaa                                                                                                                     <  1:418979/140‑1 (MQ=255)        ttCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGaa                                                                                                                     <  1:456949/140‑1 (MQ=255)              gcgGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCgg                                                                                                                             >  2:42026/1‑126 (MQ=255)              gcgGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCgg                                                                                                                             <  1:42026/126‑1 (MQ=255)                             gTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAAccc                                                                                                >  2:128326/1‑140 (MQ=255)                                 ggCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGa                                                                                            <  2:7978/140‑1 (MQ=255)                                 ggCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGa                                                                                            >  2:7255/1‑140 (MQ=255)                                       cAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAgg                                                                                      <  1:115064/140‑1 (MQ=255)                                         aTCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTg                                                                                    <  2:113386/140‑1 (MQ=255)                                         aTCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTg                                                                                    <  1:346178/140‑1 (MQ=255)                                             gTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGctct                                                                                >  2:533384/1‑140 (MQ=255)                                                             gACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTa                                                                <  1:90789/140‑1 (MQ=255)                                                                  gAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGca                                                           <  1:7255/140‑1 (MQ=255)                                                                            gCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCcgcg                                                 <  2:408667/140‑1 (MQ=255)                                                                                          ttCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtgg                                   <  1:128326/140‑1 (MQ=255)                                                                                          ttCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtgg                                   >  1:116194/1‑140 (MQ=255)                                                                                            cATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGgtggtg                                 <  1:126428/140‑1 (MQ=255)                                                                                                   gggCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgc                          >  2:57096/1‑140 (MQ=255)                                                                                                    ggCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                         >  1:519204/1‑140 (MQ=255)                                                                                                    ggCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                         >  2:197856/1‑140 (MQ=255)                                                                                                    ggCAATCTCGATCCGCAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca                         <  2:519204/140‑1 (MQ=255)                                                                                                                   cAGCGGCTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGAc          >  1:11791/1‑140 (MQ=255)                                                                                                                         cTTTCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCt    >  2:264021/1‑140 (MQ=255)                                                                                                                           ttCCAAGGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt  >  1:231183/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                      TCATTCTTTCAGCGCGGCTATGCAACCGGTTCGGCTGGCAATCTGTCGCTGCTTTTACCTGACGGGAATTTACTGGCGACACCGACAGGTTCATGCCTGGGCAATCTCGATCCGCAGCGGCTTTCCAAAGTCGCCGCGGATGGCGAATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTT  >  NC_000913/2863653‑2863914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑