Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,930,705	T→C	L377P (CTC→CCC)	sdaB →	L‑serine deaminase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,930,705	0	T	C	100.0%	110.2 / NA	32	L377P (CTC→CCC)	sdaB	L‑serine deaminase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

GTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCGAGCCGCGCGTCTGC  >  NC_000913/2930576‑2930835                                                                                                                                  |                                                                                                                                   gTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACg                                                                                                                          <  1:496480/140‑1 (MQ=255)               tGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGc                                                                                                            <  2:273132/140‑1 (MQ=255)                 ccAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCg                                                                                                          >  2:116081/1‑140 (MQ=255)                 ccAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCg                                                                                                          >  2:91246/1‑140 (MQ=255)                 ccAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCg                                                                                                          >  2:264490/1‑140 (MQ=255)                     ggTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGaca                                                                                                      <  1:278478/140‑1 (MQ=255)                       tGAAGTTGGCGTGGCGTGCTCAATGGTGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGTGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGacagg                                                                                                    <  1:275675/140‑1 (MQ=255)                          aGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGacaggtac                                                                                                 <  1:264490/140‑1 (MQ=255)                            ttGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGacaggtacag                                                                                               <  1:30574/140‑1 (MQ=255)                                cgtggcgtgCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGc                                                                                           >  1:287386/1‑140 (MQ=255)                                cgtggcgtgCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGc                                                                                           >  2:485856/1‑140 (MQ=255)                                 gtggcgtgCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCTATGGAGCACAACCCCGGTCTGGCGTGTGACCCGGTCGACCGACAGCTACAGGTGcc                                                                                          <  2:223053/140‑1 (MQ=255)                                 gtggcgtgCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGcc                                                                                          <  2:287386/140‑1 (MQ=255)                                     cgtgCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGc                                                                                      >  1:529743/1‑140 (MQ=255)                                              tGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGt                                                                             >  2:219892/1‑140 (MQ=255)                                              tGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGt                                                                             >  2:533643/1‑140 (MQ=255)                                                 cggcggcTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAAc                                                                          >  2:153959/1‑140 (MQ=255)                                                   gcggcTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGc                                                                        >  2:490806/1‑140 (MQ=255)                                                   gcggcTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGc                                                                        >  2:449384/1‑140 (MQ=255)                                                            cTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGc                                                               <  2:529743/140‑1 (MQ=255)                                                                   aaCTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTaaaa                                                        <  1:116081/140‑1 (MQ=255)                                                                     cTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGc                                                      >  2:306799/1‑140 (MQ=255)                                                                           ggCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGaa                                                <  1:490806/140‑1 (MQ=255)                                                                             cgcAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAAcg                                              >  1:408924/1‑140 (MQ=255)                                                                                              tgtgCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTg                             <  1:153959/140‑1 (MQ=255)                                                                                                tgCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTgcg                           <  1:533643/140‑1 (MQ=255)                                                                                                tgCATCGCGGCGGAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCCCCCGTATGGCGCTgct                           <  2:408924/140‑2 (MQ=255)                                                                                                       cggcggAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTAcc                    >  1:465562/1‑140 (MQ=255)                                                                                                       cggcggAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTAcc                    >  2:393605/1‑140 (MQ=255)                                                                                                         gcggAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAg                  <  1:541578/140‑1 (MQ=255)                                                                                                           ggAAATCGCCATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCg                <  1:273171/140‑1 (MQ=255)                                                                                                                    cATGGAGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCGAGCcgcgcg       <  1:485856/140‑1 (MQ=255)                                                                                                                         aGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCGAGCCGCGCGTCTGc  <  2:276883/140‑1 (MQ=255)                                                                                                                         aGCACAACCCCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCGAGCCGCGCGTCTGc  <  1:306799/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   GTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAGAACTATTAGGCGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGGAGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTACAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCAGTAAAAGCGGTGAACGCCGCACGTATGGCGCTGCGCCGTACCAGCGAGCCGCGCGTCTGC  >  NC_000913/2930576‑2930835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑