Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,054,650	A→G	V64A (GTG→GCG)	pepP ←	proline aminopeptidase P II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,054,650	0	A	G	100.0%	148.1 / NA	42	V64A (GTG→GCG)	pepP	proline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

GCGCGGTCAACGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTG  >  NC_000913/3054518‑3054780                                                                                                                                     |                                                                                                                                   gcgcGGTCAACGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATcagcgccagcac                                                                                                                             <  1:331190/140‑1 (MQ=255) gcgcGGTCAACGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATcagcgccagcac                                                                                                                             <  2:364486/140‑1 (MQ=255)  cgcgGTCAACGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCTCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCagcgccagcacc                                                                                                                            >  1:117926/1‑140 (MQ=255)  cgcgGTCAACGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCagcgccagcacc                                                                                                                            <  2:137441/140‑1 (MQ=255)    cgGTCAACGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGc                                                                                                                          >  2:429182/1‑140 (MQ=255)          aCGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCgg                                                                                                                    >  1:255634/1‑140 (MQ=255)          aCGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCgg                                                                                                                    <  2:423783/140‑1 (MQ=255)                      tctGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGcc                                                                                                        <  1:421758/140‑1 (MQ=255)                        tGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCgg                                                                                                      <  1:429182/140‑1 (MQ=255)                                    cTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGa                                                                                          >  1:489577/1‑140 (MQ=255)                                    cTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGAACCAGa                                                                                          <  2:61250/140‑1 (MQ=255)                                       gCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGt                                                                                       <  2:225178/140‑1 (MQ=255)                                            aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTg                                                                                  >  2:396565/1‑140 (MQ=255)                                            aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTg                                                                                  >  2:437575/1‑140 (MQ=255)                                            aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTg                                                                                  >  1:433685/1‑140 (MQ=255)                                            aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTg                                                                                  >  1:462924/1‑140 (MQ=255)                                            aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTg                                                                                  >  2:265461/1‑140 (MQ=255)                                                aCGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCt                                                                              <  2:140310/140‑1 (MQ=255)                                                 cGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTc                                                                               <  1:160259/138‑1 (MQ=255)                                                 cGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTc                                                                               >  2:160259/1‑138 (MQ=255)                                                   gCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGAc                                                                           <  2:492347/140‑1 (MQ=255)                                                   gCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGAc                                                                           >  2:368987/1‑140 (MQ=255)                                                     cAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGa                                                                         <  1:403175/140‑1 (MQ=255)                                                          cAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAggg                                                                    <  1:358622/140‑1 (MQ=255)                                                             aTCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGa                                                                            <  1:252696/129‑1 (MQ=255)                                                             aTCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGa                                                                            >  2:252696/1‑129 (MQ=255)                                                                 ccgccgTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCg                                                             <  2:210799/140‑1 (MQ=255)                                                                 ccgccgTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCg                                                             <  2:393660/140‑1 (MQ=255)                                                                  cgccgTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGc                                                            >  1:400519/1‑140 (MQ=255)                                                                  cgccgTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGc                                                            >  2:321845/1‑140 (MQ=255)                                                                          ggTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGgcg                                                    <  2:255634/140‑1 (MQ=255)                                                                            tCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCt                                                  <  2:433685/140‑1 (MQ=255)                                                                             ctcgAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCCCCGCTTCCGGTTCGTTAAAGCCGGTGAAGTCCCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCCCtc                                                 <  2:117926/138‑2 (MQ=255)                                                                             cgcgAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTa                                                 <  2:236120/140‑1 (MQ=255)                                                                                gAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACgt                                              <  2:489577/140‑1 (MQ=255)                                                                                   cgcgGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGtt                                           <  1:368987/140‑1 (MQ=255)                                                                                             cAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTg                                 <  2:320298/140‑1 (MQ=255)                                                                                                     tgtgGTTATGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCaaaa                         >  2:83037/1‑140 (MQ=255)                                                                                                            aTGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAgcg                  <  1:83037/140‑1 (MQ=255)                                                                                                             tGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAgcgc                 >  2:399123/1‑140 (MQ=255)                                                                                                             tGAGTGTCATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAgcgc                 >  1:72629/1‑140 (MQ=255)                                                                                                                    cATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTg          >  2:48263/1‑140 (MQ=255)                                                                                                                    cATCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTg          >  2:483861/1‑140 (MQ=255)                                                                                                                     aTCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGc         <  2:400519/140‑1 (MQ=255)                                                                                                                     aTCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGc         <  1:396565/140‑1 (MQ=255)                                                                                                                     aTCGCTTTTAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGc         >  2:47188/1‑140 (MQ=255)                                                                                                                            ttAATCAGCGCCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTg  >  2:156626/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   GCGCGGTCAACGCCCAGTTTCTCTGGCGCGGCATCCTGGCCTAAGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTG  >  NC_000913/3054518‑3054780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑