Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,950	T→C	Y202H (TAT→CAT)	endA →	DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,950	0	T	C	100.0%	122.0 / NA	35	Y202H (TAT→CAT)	endA	DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/21);  total (14/21)

TTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTT  >  NC_000913/3090814‑3091080                                                                                                                                         |                                                                                                                                   tttCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATc                                                                                                                                 <  2:62928/140‑1 (MQ=255)              gCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTg                                                                                                                    <  1:253037/140‑1 (MQ=255)               cTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTgg                                                                                                                   <  1:202563/140‑1 (MQ=255)                               gcgTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATg                                                                                                   >  2:543539/1‑140 (MQ=255)                                 gTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGaa                                                                                                 >  1:162929/1‑140 (MQ=255)                                  tGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAAc                                                                                                >  2:394377/1‑140 (MQ=255)                                           tGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAATCTGACACTCTTTCGCAAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa                                                                                       <  2:507874/140‑1 (MQ=255)                                           tGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa                                                                                       <  1:543539/140‑1 (MQ=255)                                                 tGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGc                                                                                 >  1:214162/1‑140 (MQ=255)                                                     cgcACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAggg                                                                             >  2:263514/1‑140 (MQ=255)                                                       cACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCa                                                                           <  1:528991/140‑1 (MQ=255)                                                       cACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCa                                                                           <  1:28979/140‑1 (MQ=255)                                                         ccTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt                                                                         <  2:514184/140‑1 (MQ=255)                                                                cTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAAcc                                                                  <  1:394377/140‑1 (MQ=255)                                                                  atatGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCg                                                                >  2:356308/1‑140 (MQ=255)                                                                   tatGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGt                                                               <  1:263514/140‑1 (MQ=255)                                                                        gcgACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTAtgtg                                                          >  2:505622/1‑140 (MQ=255)                                                                        gcgACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTAtgtg                                                          >  2:72222/1‑140 (MQ=255)                                                                                     ccTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGcc                                             <  1:263172/140‑1 (MQ=255)                                                                                          cacTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgcg                                        >  2:71688/1‑140 (MQ=255)                                                                                          cacTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgcg                                        >  2:26283/1‑140 (MQ=255)                                                                                               tctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGaaa                                   >  2:104796/1‑140 (MQ=255)                                                                                               tctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGaaa                                   >  2:296419/1‑140 (MQ=255)                                                                                               tctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGaaa                                   >  2:33029/1‑140 (MQ=255)                                                                                               tctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGaaa                                   >  2:404449/1‑140 (MQ=255)                                                                                                  cGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAagag                                <  1:33029/140‑1 (MQ=255)                                                                                                   gCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGc                               <  1:91124/140‑1 (MQ=255)                                                                                                     cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTa                             <  1:356308/140‑1 (MQ=255)                                                                                                                         cGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCt         <  2:214162/140‑1 (MQ=255)                                                                                                                         cGCATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCt         <  1:296419/140‑1 (MQ=255)                                                                                                                           cATGGAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCttt       <  2:503180/140‑1 (MQ=255)                                                                                                                              ggAACAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTg    <  2:162929/140‑1 (MQ=255)                                                                                                                                aaCAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGtt  <  1:300690/140‑1 (MQ=255)                                                                                                                                aaCAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGtt  <  1:72222/140‑1 (MQ=255)                                                                                                                                aaCAAGATGCATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGtt  <  1:26283/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TTTCAAAGAAAAAGCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAACCTACACTAGCGGGATTCTTTTTGTT  >  NC_000913/3090814‑3091080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑