Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,118	T→C	F9S (TTC→TCC)	yggX →	putative Fe(2(+))‑trafficking protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,118	0	T	C	100.0%	63.8 / NA	19	F9S (TTC→TCC)	yggX	putative Fe(2(+))‑trafficking protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/10);  total (9/10)

CGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGA  >  NC_000913/3103989‑3104240                                                                                                                                  |                                                                                                                           cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGt                                                                                                                  >  1:359252/1‑140 (MQ=255)                    ccTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGAtt                                                                                               <  1:429820/140‑1 (MQ=255)                         cGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAgg                                                                                                  <  1:278248/132‑1 (MQ=255)                         cGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAgg                                                                                                  >  2:278248/1‑132 (MQ=255)                                        gTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAg                                                                           <  2:249276/140‑1 (MQ=255)                                          ttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCt                                                                         <  1:63169/140‑1 (MQ=255)                                                      ttACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGc                                                                              <  1:15722/123‑1 (MQ=255)                                                      ttACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGc                                                                              >  2:15722/1‑123 (MQ=255)                                                             cTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAAc                                                      <  2:260707/140‑1 (MQ=255)                                                                gcgcgcCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag                                                   <  1:471908/140‑1 (MQ=255)                                                                gcgcgcCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag                                                   >  1:320145/1‑140 (MQ=255)                                                                                  tGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag                                                   <  1:8219/122‑1 (MQ=255)                                                                                  tGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag                                                   >  2:8219/1‑122 (MQ=255)                                                                                               agGATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGgcagc                    >  1:295919/1‑140 (MQ=255)                                                                                                ggATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGgcagca                   >  1:357814/1‑140 (MQ=255)                                                                                                ggATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGgcagca                   <  2:123355/140‑1 (MQ=255)                                                                                                ggATGATTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGgcagca                   <  2:18351/140‑1 (MQ=255)                                                                                                  atgatTTATGAGCAGAACGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGcaca                 >  1:331009/1‑140 (MQ=255)                                                                                                                 aaCGATTTTTTGTACTTCCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGa  >  2:438235/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                           CGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGA  >  NC_000913/3103989‑3104240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑