Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,110,037	T→C	I162T (ATC→ACC)	yqgA →	DUF554 domain‑containing protein YqgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,110,037	0	T	C	100.0%	111.1 / NA	32	I162T (ATC→ACC)	yqgA	DUF554 domain‑containing protein YqgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

ACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTACGCATCTGTG  >  NC_000913/3109904‑3110171                                                                                                                                      |                                                                                                                                       aCCGGGATCTTCGGGGCGATGAACGAAGGGATGCCCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCAtt                                                                                                                                  <  1:395174/140‑1 (MQ=255)  ccGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTa                                                                                                                                 >  2:359615/1‑140 (MQ=255)          ttCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGatcat                                                                                                                         <  2:226137/140‑1 (MQ=255)                 cGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTa                                                                                                                  >  1:75596/1‑140 (MQ=255)                 cGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTa                                                                                                                  <  1:325355/140‑1 (MQ=255)                              gATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCa                                                                                                                      >  2:474906/1‑123 (MQ=255)                              gATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCa                                                                                                                      <  1:474906/123‑1 (MQ=255)                                   ccGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGcc                                                                                                >  2:308956/1‑140 (MQ=255)                                        gATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCt                                                                                           >  1:90831/1‑140 (MQ=255)                                             gAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGAttt                                                                                      <  1:308956/140‑1 (MQ=255)                                                tATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTAc                                                                                   <  1:359615/140‑1 (MQ=255)                                                    ttAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCt                                                                               <  2:123073/140‑1 (MQ=255)                                                        tCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGAcc                                                                           >  2:24417/1‑140 (MQ=255)                                                                   tttCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCgat                                                                <  1:545161/140‑1 (MQ=255)                                                                   tttCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCgat                                                                <  1:94685/140‑1 (MQ=255)                                                                        tGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATgg                                                           <  1:24417/140‑1 (MQ=255)                                                                          aTTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCa                                                         <  1:200909/140‑1 (MQ=255)                                                                                        atgatCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGt                                           >  1:144098/1‑140 (MQ=255)                                                                                           atCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAgg                                        <  1:214648/140‑1 (MQ=255)                                                                                                cGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGtt                                   >  1:489131/1‑140 (MQ=255)                                                                                                       tCGCTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATtgct                            >  1:13155/1‑140 (MQ=255)                                                                                                          cTGGGTATTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTgctgct                         >  2:336542/1‑140 (MQ=255)                                                                                                                aTTGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAAc                   <  2:75596/140‑1 (MQ=255)                                                                                                                  tGCGGTATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCg                 >  2:395320/1‑140 (MQ=255)                                                                                                                       tATCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTa            >  1:33175/1‑140 (MQ=255)                                                                                                                        aTCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTAc           >  2:490221/1‑140 (MQ=255)                                                                                                                         tCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCaa                    >  2:117007/1‑130 (MQ=255)                                                                                                                         tCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCaa                    <  1:117007/130‑1 (MQ=255)                                                                                                                         tCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTACg          >  1:192434/1‑140 (MQ=255)                                                                                                                         tCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTACg          >  2:347988/1‑140 (MQ=255)                                                                                                                         tCGGTGATTAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTACg          >  1:153027/1‑140 (MQ=255)                                                                                                                                 tAGTACCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTACGCATCtgtg  <  1:395320/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGATGATGGCAGACTTCAGCGCCGTAGGCGGTTTATTGCTGCTGGCAACCGGATTACGCATCTGTG  >  NC_000913/3109904‑3110171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑