Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,177,926	A→G	intergenic (‑16/‑189)	nudF ← / → tolC	ADP‑sugar pyrophosphatase/outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,926	0	A	G	100.0%	129.0 / NA	37	intergenic (‑16/‑189)	nudF/tolC	ADP‑sugar pyrophosphatase/outer membrane channel TolC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

GACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCG  >  NC_000913/3177799‑3178060                                                                                                                                |                                                                                                                                       gACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAAt                                                                                                                            <  1:439643/140‑1 (MQ=255)  aCGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAAtt                                                                                                                           <  2:298737/140‑1 (MQ=255)        tCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAAtgt                                                                                                                     >  1:121366/1‑140 (MQ=255)                  tCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGc                                                                                                           >  1:285315/1‑140 (MQ=255)                    tAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGa                                                                                                         >  2:302866/1‑140 (MQ=255)                    tAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGa                                                                                                         >  2:491817/1‑140 (MQ=255)                      aTGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACg                                                                                                       <  2:343498/140‑1 (MQ=255)                           aaaaaGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCTAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGa                                                                                                  >  1:215759/1‑140 (MQ=255)                           aaaaaGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTg                                                                                                   >  1:246680/1‑139 (MQ=255)                           aaaaaGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTg                                                                                                   <  2:246680/139‑1 (MQ=255)                           aaaaaGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGa                                                                                                  >  1:146701/1‑140 (MQ=255)                           aaaaaGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGa                                                                                                  >  2:500274/1‑140 (MQ=255)                                      aTAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGCGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGa                                                                                       <  1:318115/140‑1 (MQ=255)                                         aaGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCa                                                                                    <  2:179743/140‑1 (MQ=255)                                                   cGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGtt                                                                          <  2:56284/140‑1 (MQ=255)                                                   cGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGtt                                                                          >  1:367919/1‑140 (MQ=255)                                                   cGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCGAATAGTGAAGTAAATGTGAATTTCAGCGACGTTTGACTGCCGTGTGAGCAGTCATGTGtt                                                                          >  2:487176/1‑140 (MQ=255)                                                    gTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTa                                                                         >  2:27225/1‑140 (MQ=255)                                                              ttCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGca                                                               <  1:500274/140‑1 (MQ=255)                                                              ttCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGca                                                               <  1:302866/140‑1 (MQ=255)                                                              ttCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGca                                                               <  2:440961/140‑1 (MQ=255)                                                                   cATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTa                                                          >  2:459375/1‑140 (MQ=255)                                                                    aTCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAAGGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTaa                                                         <  1:405764/140‑1 (MQ=255)                                                                      cGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACg                                                       <  2:325766/140‑1 (MQ=255)                                                                                  aTGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACg                                           <  2:367919/140‑1 (MQ=255)                                                                                             aGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAAcc                                >  1:546029/1‑140 (MQ=255)                                                                                                       gCTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTa                      <  2:206122/140‑1 (MQ=255)                                                                                                        cTTAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAg                     <  2:146701/140‑1 (MQ=255)                                                                                                          tAAGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCg                   <  1:478929/140‑1 (MQ=255)                                                                                                            aGCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGc                 <  1:193589/140‑1 (MQ=255)                                                                                                             gCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGc                 <  2:8979/139‑1 (MQ=255)                                                                                                             gCATTGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGc                 >  1:8979/1‑139 (MQ=255)                                                                                                                 tGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGc                 >  2:182981/1‑135 (MQ=255)                                                                                                                 tGTTAATGTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGc                 <  1:182981/135‑1 (MQ=255)                                                                                                                        gTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAAt     >  2:161714/1‑140 (MQ=255)                                                                                                                        gTCCTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAAt     >  2:13868/1‑140 (MQ=255)                                                                                                                           cTGGCGCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCg  >  2:532241/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                       GACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCG  >  NC_000913/3177799‑3178060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑