Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,574	A→G	N54D (AAC→GAC)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,574	0	A	G	100.0%	210.2 / NA	59	N54D (AAC→GAC)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/24);  total (35/24)

TAATGATATTAGTAGTGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTAACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTAT  >  NC_000913/3268438‑3268707                                                                                                                                         |                                                                                                                                      tAATGATATTAGTAGTGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGaca                                                                                                                                    >  1:527515/1‑140 (MQ=255) tAATGATATTAGTAGTGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGaca                                                                                                                                    <  2:289448/140‑1 (MQ=255)           agtagtGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAAcc                                                                                                                          >  2:467945/1‑140 (MQ=255)               gtGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTa                                                                                                                               >  2:228450/1‑131 (MQ=255)               gtGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTa                                                                                                                               <  1:228450/131‑1 (MQ=255)               gtGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCtggt                                                                                                                      >  1:35964/1‑140 (MQ=255)               gtGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCtggt                                                                                                                      <  1:102897/140‑1 (MQ=255)                 gATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCtggtgg                                                                                                                    >  2:64566/1‑140 (MQ=255)                 gATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCtggtgg                                                                                                                    >  2:207293/1‑140 (MQ=255)                  aTTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGa                                                                                                                   >  2:34159/1‑140 (MQ=255)                  aTTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGa                                                                                                                   >  2:537759/1‑140 (MQ=255)                     tAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAAc                                                                                                                           >  2:330034/1‑129 (MQ=255)                     tAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAAc                                                                                                                           <  1:330034/129‑1 (MQ=255)                     tAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGaaaa                                                                                                                <  1:467945/140‑1 (MQ=255)                      aGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGGATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAAt                                                                                                               >  1:125820/1‑140 (MQ=255)                       gTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATg                                                                                                              >  2:169574/1‑140 (MQ=255)                           gTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCAtt                                                                                                          <  1:291798/140‑1 (MQ=255)                            tCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTa                                                                                                         >  2:137187/1‑140 (MQ=255)                                    tgaatgaCCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGt                                                                                                 <  1:177316/140‑1 (MQ=255)                                         gaCCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGAttt                                                                                            >  2:98660/1‑140 (MQ=255)                                              tCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCaaa                                                                                       <  2:26982/140‑1 (MQ=255)                                              tCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCaaa                                                                                       <  2:374139/140‑1 (MQ=255)                                              tCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCaaa                                                                                       <  1:187015/140‑1 (MQ=255)                                              tCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCaaa                                                                                       <  1:70073/140‑1 (MQ=255)                                                   aaaGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGaa                                                                                  >  2:442160/1‑140 (MQ=255)                                                    aaGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAAt                                                                                 <  1:207293/140‑1 (MQ=255)                                                      gCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTa                                                                               <  1:99567/140‑1 (MQ=255)                                                          aaCAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGt                                                                           >  1:25234/1‑140 (MQ=255)                                                            cAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTAc                                                                         >  1:102338/1‑140 (MQ=255)                                                                  aTAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACaa                                                                   >  1:360775/1‑140 (MQ=255)                                                                      ccTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATg                                                               <  1:537759/140‑1 (MQ=255)                                                                        tCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATCATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTa                                                             <  2:373826/140‑1 (MQ=255)                                                                         cGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTa                                                                      <  2:507626/130‑1 (MQ=255)                                                                         cGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTa                                                                      >  1:507626/1‑130 (MQ=255)                                                                          gAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAAt                                                           <  1:137187/140‑1 (MQ=255)                                                                           aaGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAAtt                                                          <  1:442160/140‑1 (MQ=255)                                                                                cTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTta                                                     >  2:111074/1‑140 (MQ=255)                                                                                 tAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCCTtat                                                    >  1:319502/1‑140 (MQ=255)                                                                                  aGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTtata                                                   <  2:19527/140‑1 (MQ=255)                                                                                              aGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATgtctcgtctc                                       >  2:458357/1‑140 (MQ=255)                                                                                              aGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATgtctcgtctc                                       >  2:191069/1‑140 (MQ=255)                                                                                                   aaCTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGt                                  >  1:97841/1‑140 (MQ=255)                                                                                                    aCTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTc                                 >  1:155541/1‑140 (MQ=255)                                                                                                    aCTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTc                                 >  2:206786/1‑140 (MQ=255)                                                                                                     cTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGt                                  <  1:343686/138‑1 (MQ=255)                                                                                                     cTAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGt                                  >  2:343686/1‑138 (MQ=255)                                                                                                      tAAAACAGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTg                               <  1:230977/140‑1 (MQ=255)                                                                                                            aGACCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGAtttt                         <  1:311306/140‑1 (MQ=255)                                                                                                              aCCTCTATTGAAATGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGAtttttt                       >  2:241670/1‑140 (MQ=255)                                                                                                                          aTGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGc           >  2:170024/1‑140 (MQ=255)                                                                                                                          aTGGTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGc           >  2:529272/1‑140 (MQ=255)                                                                                                                             gTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCa        >  2:403995/1‑140 (MQ=255)                                                                                                                             gTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCa        >  1:283567/1‑140 (MQ=255)                                                                                                                             gTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCa        >  2:457056/1‑140 (MQ=255)                                                                                                                             gTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCa        >  1:337428/1‑140 (MQ=255)                                                                                                                             gTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCa        >  1:45608/1‑140 (MQ=255)                                                                                                                             gTCACTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCa        >  1:430872/1‑140 (MQ=255)                                                                                                                                aCTTTACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAat     <  2:102338/140‑1 (MQ=255)                                                                                                                                   ttACTTGACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAattat  <  1:31595/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TAATGATATTAGTAGTGATTTAGTCCGTCGAAAAATGAATGACCTTCCTGAAAGCCCAACAGGAAATAACCTCGAAGCACTAGCACCAGGTATAGAAAAACTAAAACAGACCTCTATTGAAATGGTCACTTTACTTAACACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTAT  >  NC_000913/3268438‑3268707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑