Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,273,214	T→C	Y115C (TAC→TGC)	garL ←	alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,273,214	0	T	C	100.0%	70.4 / NA	22	Y115C (TAC→TGC)	garL	alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

GCCCTGCTGACTTTCTATCTGGACCAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCG  >  NC_000913/3273087‑3273335                                                                                                                                |                                                                                                                          gCCCTGCTGACTTTCTATCTGGACCAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATg                                                                                                               >  1:377489/1‑140 (MQ=255)    ctgctgACTTTCTATCTGGACCAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCa                                                                                                            <  2:243553/140‑1 (MQ=255)                 aTCTGGACCAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGc                                                                                               >  2:136688/1‑140 (MQ=255)                    tGGACCAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTc                                                                                            >  2:330542/1‑140 (MQ=255)                      gACCAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCct                                                                                          >  2:212104/1‑140 (MQ=255)                         cAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCtt                                                                                       <  1:497755/140‑1 (MQ=255)                            aaTAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTg                                                                                    >  1:338123/1‑140 (MQ=255)                                gTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTc                                                                                >  2:428563/1‑140 (MQ=255)                                      ttCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACaaa                                                                          >  2:542814/1‑140 (MQ=255)                                      ttCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACaaa                                                                          >  1:331492/1‑140 (MQ=255)                                      ttCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACaaa                                                                          >  1:329526/1‑140 (MQ=255)                                        cTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGtt                                                                                  <  2:27276/130‑1 (MQ=255)                                        cTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGtt                                                                                  >  1:27276/1‑130 (MQ=255)                                            ttCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAAt                                                                    <  2:233349/140‑1 (MQ=255)                                              cGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCa                                                                  <  1:428563/140‑1 (MQ=255)                                              cGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCa                                                                  <  1:242846/140‑1 (MQ=255)                                                aCTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAgg                                                                <  2:10534/140‑1 (MQ=255)                                                                   cACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGatat                                             <  2:338123/140‑1 (MQ=255)                                                                              aCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGc                                  <  2:331492/140‑1 (MQ=255)                                                                                              agaAACGGAGACGCCGCGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCt                  <  2:417427/140‑1 (MQ=255)                                                                                                          gccgcGAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCa      >  2:487269/1‑140 (MQ=255)                                                                                                              cgAATGCCTTCCGGTGGGCAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCg  <  1:136688/140‑1 (MQ=255)                                                                                                                                |                                                                                                                          GCCCTGCTGACTTTCTATCTGGACCAGAATAGTGATGTTCTTGTTCGACTGAGCGAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCG  >  NC_000913/3273087‑3273335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑