Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,306,971 T→C D297G (GAC→GGC)  deaD ← ATP‑dependent RNA helicase DeaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,306,9710TC100.0% 145.3 / NA 41D297G (GAC→GGC) deaDATP‑dependent RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/21);  total (20/21)

CCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAAC  >  NC_000913/3306843‑3307107
                                                                                                                                |                                                                                                                                        
ccGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCa                                                                                                                               >  2:189707/1‑140 (MQ=255)
ccGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCa                                                                                                                               >  2:49531/1‑140 (MQ=255)
        ccGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAg                                                                                                                       <  1:538884/140‑1 (MQ=255)
         cGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAgg                                                                                                                      <  2:143409/140‑1 (MQ=255)
         cGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAgg                                                                                                                      <  2:364281/140‑1 (MQ=255)
          ggTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTAAGGc                                                                                                                     <  2:376688/140‑1 (MQ=255)
            tACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATGTCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGAAGACCATCTTTCAGGCGt                                                                                                                   >  2:484286/1‑140 (MQ=255)
                 ccGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAg                                                                                                              <  1:38534/140‑1 (MQ=255)
                  cGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAgt                                                                                                             >  1:357245/1‑140 (MQ=255)
                   gATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAgtg                                                                                                            <  1:101332/140‑1 (MQ=255)
                         gTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAgtg                                                                                                            <  2:30189/134‑1 (MQ=255)
                         gTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAgtg                                                                                                            >  1:30189/1‑134 (MQ=255)
                           gAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAgtg                                                                                                            <  1:186750/132‑1 (MQ=255)
                           gAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAgtg                                                                                                            >  2:186750/1‑132 (MQ=255)
                            aaCGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCAc                                                                                                   >  2:400835/1‑140 (MQ=255)
                            aaCGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCAc                                                                                                   <  1:521450/140‑1 (MQ=255)
                              cGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGc                                                                                                 <  2:513323/140‑1 (MQ=255)
                                    aCTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGcc                                                                                           <  1:400835/140‑1 (MQ=255)
                                      tCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTg                                                                                         >  2:158248/1‑140 (MQ=255)
                                           aTCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCa                                                                                    <  1:370447/140‑1 (MQ=255)
                                                    gATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGt                                                                           >  1:322363/1‑140 (MQ=255)
                                                              ttAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCgcggc                                                                 <  1:336011/140‑1 (MQ=255)
                                                              ttAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCgcggc                                                                 >  2:383337/1‑140 (MQ=255)
                                                                      cAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCa                                                                        <  1:219406/125‑1 (MQ=255)
                                                                      cAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCa                                                                        >  2:219406/1‑125 (MQ=255)
                                                                             aTACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGtt                                                  >  2:363217/1‑140 (MQ=255)
                                                                              tACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTa                                                 >  1:34245/1‑140 (MQ=255)
                                                                                    cAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCa                                           <  1:98647/140‑1 (MQ=255)
                                                                                      aCGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAag                                         <  1:189707/140‑1 (MQ=255)
                                                                                       cGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCTTGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAaga                                        >  1:514756/1‑140 (MQ=255)
                                                                                             ggCCACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCa                                  >  1:445130/1‑140 (MQ=255)
                                                                                                cACGGGCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGcc                               <  1:158248/140‑1 (MQ=255)
                                                                                                   gggCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACt                            >  1:281534/1‑140 (MQ=255)
                                                                                                   gggCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCACGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGc                                >  2:287802/1‑136 (MQ=255)
                                                                                                   gggCTGCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCACGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGc                                <  1:287802/136‑1 (MQ=255)
                                                                                                        gCAACGTCGGTCGCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCag                       >  1:347314/1‑140 (MQ=255)
                                                                                                              tcggtcgCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTcgc                 >  2:520037/1‑140 (MQ=255)
                                                                                                                ggtcgCAATCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGt               >  1:25903/1‑140 (MQ=255)
                                                                                                                        tCAGGATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTa       <  1:314273/140‑1 (MQ=255)
                                                                                                                           ggATGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacga    <  1:520037/140‑1 (MQ=255)
                                                                                                                             aTGCCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac  <  2:357245/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                        
CCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGCTGCAACGTCGGTCGCAATCAGGATGTCCAGACGACCATCTTTCAGGCGTTCCAGTGTCTGTTCACGCAGCGCCTGGTTCATGTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAAC  >  NC_000913/3306843‑3307107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑