Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,347,113	A→G	intergenic (+44/‑2)	ptsN → / → rapZ	phosphotransferase system enzyme IIA(Ntr)/RNase adaptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,347,113	0	A	G	100.0%	78.5 / NA	23	intergenic (+44/‑2)	ptsN/rapZ	phosphotransferase system enzyme IIA(Ntr)/RNase adaptor protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

CCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCT  >  NC_000913/3346988‑3347237                                                                                                                              |                                                                                                                             ccgccgTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACtgatga                                                                                                                <  1:528644/140‑1 (MQ=255)  cgccgTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTgatgat                                                                                                               >  2:75343/1‑140 (MQ=255)           cgcgCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCgg                                                                                                      <  1:75343/140‑1 (MQ=255)            gcgcAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGa                                                                                                     <  1:44217/140‑1 (MQ=255)                aGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACgtt                                                                                                 <  2:450130/140‑1 (MQ=255)                     agagCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACgttcaggt                                                                                            >  2:363235/1‑140 (MQ=255)                            tGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTa                                                                                     >  2:85559/1‑140 (MQ=255)                            tGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTa                                                                                     >  2:498176/1‑140 (MQ=255)                            tGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTAGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTa                                                                                     >  2:329950/1‑140 (MQ=255)                               agagCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAAt                                                                                  <  1:329950/140‑1 (MQ=255)                                 agCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCt                                                                                <  1:498176/140‑1 (MQ=255)                                           aaTCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCtgc                                                                      <  1:539894/140‑1 (MQ=255)                                                ttACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCtgcgtgcg                                                                 >  1:45669/1‑140 (MQ=255)                                                          cGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGata                                                       <  1:293873/140‑1 (MQ=255)                                                             aGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATgg                                                    >  1:169452/1‑140 (MQ=255)                                                                    ccGGATGAAGCGTAGTTATTCTGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATggg                                                   >  2:386060/1‑134 (MQ=255)                                                                    ccGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATggg                                                   <  1:386060/134‑1 (MQ=255)                                                                      ggATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACt                                           <  1:36077/140‑1 (MQ=255)                                                                      ggATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACt                                           <  2:69329/140‑1 (MQ=255)                                                                                        cGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCg                         >  1:122430/1‑140 (MQ=255)                                                                                                              tgtgAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGAt        <  1:417688/135‑1 (MQ=255)                                                                                                              tgtgAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGAt        >  2:417688/1‑135 (MQ=255)                                                                                                               gtgAGGAGAAACAGTGCATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCt  <  2:461379/140‑1 (MQ=255)                                                                                                                              |                                                                                                                             CCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAACAGTACATGGTACTGATGATCGTCAGCGGACGTTCAGGTTCAGGTAAATCTGTCGCCCTGCGTGCGCTGGAAGATATGGGTTTTTACTGCGTGGATAACCTTCCCGTAGTGTTGTTACCCGATCTGGCT  >  NC_000913/3346988‑3347237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑