Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,987	T→C	V40A (GTC→GCC)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,987	0	T	C	100.0%	80.1 / NA	24	V40A (GTC→GCC)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/15);  total (9/15)

CACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTG  >  NC_000913/3374855‑3375117                                                                                                                                     |                                                                                                                                   cacGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTa                                                                                                                             <  2:78562/140‑1 (MQ=255)     gAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGt                                                                                                                               <  1:272514/134‑1 (MQ=255)     gAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGt                                                                                                                               >  2:272514/1‑134 (MQ=255)              aaTGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGgtggtg                                                                                                                <  2:217/140‑1 (MQ=255)                  ttCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACt                                                                                                            >  1:401878/1‑140 (MQ=255)                    cGGCATAATTATATCTGTCATCGTATCAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCg                                                                                                          <  1:380756/140‑1 (MQ=255)                     ggCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGa                                                                                                         >  1:356018/1‑140 (MQ=255)                      gCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGAt                                                                                                        <  1:390920/140‑1 (MQ=255)                       cATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATc                                                                                                       >  2:132531/1‑140 (MQ=255)                                   tGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAagca                                                                                           <  1:19311/140‑1 (MQ=255)                                    gTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAagcag                                                                                          <  2:384836/140‑1 (MQ=255)                                    gTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAagcag                                                                                          <  2:163305/140‑1 (MQ=255)                                     tCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAagcagc                                                                                         <  1:347294/140‑1 (MQ=255)                                               aattaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGa                                                                               >  2:223724/1‑140 (MQ=255)                                                            tATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTta                                                                  <  2:105116/140‑1 (MQ=255)                                                                 gATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATg                                                             <  2:356018/140‑1 (MQ=255)                                                                        aaaaaCTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCt                                                      >  2:407794/1‑140 (MQ=255)                                                                                                ctggctgCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGc                              <  1:407794/140‑1 (MQ=255)                                                                                                ctggctgCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGc                              <  1:266946/140‑1 (MQ=255)                                                                                                  ggctgCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCg                                    <  1:34912/132‑1 (MQ=255)                                                                                                  ggctgCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCg                                    >  2:34912/1‑132 (MQ=255)                                                                                                          cAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTgggg                    <  1:506264/140‑1 (MQ=255)                                                                                                                  ttCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGAt            >  1:276595/1‑140 (MQ=255)                                                                                                                            tgtgCGGTGCCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTg  >  2:184115/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   CACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATATTTATAATGAAATCCTGCGCATGCTGTCCAACCGCATTGCCGGATTGGGGCTGTCGATTATGGCGGTG  >  NC_000913/3374855‑3375117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑