Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	412,685	A→G	R1023R (CGT→CGC)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	412,685	0	A	G	100.0%	108.7 / NA	31	R1023R (CGT→CGC)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

AGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAGGAACAGC  >  NC_000913/412555‑412817                                                                                                                                   |                                                                                                                                     aGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTc                                                                                                                             >  2:513277/1‑140 (MQ=255)        ccAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCtt                                                                                                                      <  2:255806/140‑1 (MQ=255)                 aaTTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCAt                                                                                                                           <  1:340935/126‑1 (MQ=255)                 aaTTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCAt                                                                                                                           >  2:340935/1‑126 (MQ=255)                      gCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATc                                                                                                        >  2:282383/1‑140 (MQ=255)                            ctGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCg                                                                                                  <  1:3050/140‑1 (MQ=255)                                    aTTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCg                                                                                                  >  2:516641/1‑132 (MQ=255)                                    aTTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCg                                                                                                  <  1:516641/132‑1 (MQ=255)                                      tATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGc                                                                                        >  2:443165/1‑140 (MQ=255)                                         ccTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCAc                                                                                     >  1:33218/1‑140 (MQ=255)                                         ccTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCAc                                                                                     >  1:439787/1‑140 (MQ=255)                                         ccTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAG‑‑CAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTg                                                                                   >  2:151150/1‑140 (MQ=255)                                               gAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGt                                                                               <  1:282383/140‑1 (MQ=255)                                                          tCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATcc                                                                    >  1:327094/1‑140 (MQ=255)                                                          tCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATcc                                                                    >  1:261878/1‑140 (MQ=255)                                                          tCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATcc                                                                    >  1:210413/1‑140 (MQ=255)                                                               gCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAgcgc                                                               >  1:271362/1‑140 (MQ=255)                                                                      tACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAgg                                                        <  2:261878/140‑1 (MQ=255)                                                                       aCTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAggg                                                       <  1:178089/140‑1 (MQ=255)                                                                                 ttGCTGTAG‑‑CAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCg                                           <  1:151150/140‑1 (MQ=255)                                                                                   gCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCg                                           <  1:290869/140‑1 (MQ=255)                                                                                     tGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGtt                                         >  2:268626/1‑140 (MQ=255)                                                                                               gCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCa                               >  2:520511/1‑140 (MQ=255)                                                                                                            ttCACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAAcc                  <  2:33218/140‑1 (MQ=255)                                                                                                              cACTTTGATCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCtt                <  2:439787/140‑1 (MQ=255)                                                                                                                     aTCTGCACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAg         >  1:268121/1‑140 (MQ=255)                                                                                                                          cACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAGGAACa    <  2:327094/140‑1 (MQ=255)                                                                                                                          cACCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAGGAACa    <  2:351512/140‑1 (MQ=255)                                                                                                                           aCCGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAGGAACAg   <  2:271362/140‑1 (MQ=255)                                                                                                                            ccGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAGGAACAGc  <  1:513277/140‑1 (MQ=255)                                                                                                                            ccGGAATGCGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAGGAACAGc  <  1:268626/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     AGCAAGGCCAAGAGATAATTCGCCCCTCTGTATTCATTATCCTGCTGAATAGTTATTTCACTGCAAACGTACTTTCCAGTTTGCTGTAGCCCAGGCCGTTGATCTTTTTCACTTTGATCTGCACCGGAATACGCTCTTTCATCGCTTCTACGTGGCTAATCACACCGATGGTTTTGCCACTGGCGTTCAGGGCATCCAGCGCATCAAGGGCGGTATCCAGCGTTTCGCTATCCAGCGTGCCAAAACCTTCATCAAGGAACAGC  >  NC_000913/412555‑412817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑