Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,726	A→G	V134A (GTT→GCT)	aaeA ←	aromatic carboxylic acid efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,726	0	A	G	100.0%	163.1 / NA	46	V134A (GTT→GCT)	aaeA	aromatic carboxylic acid efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/21);  total (25/21)

CCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTGC  >  NC_000913/3388591‑3388857                                                                                                                                        |                                                                                                                                    ccTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGt                                                                                                                                 <  1:5904/140‑1 (MQ=255)    cGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTg                                                                                                                              <  2:303970/140‑1 (MQ=255)         aataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                         <  2:28820/140‑1 (MQ=255)         aataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                         <  2:411075/140‑1 (MQ=255)         aataaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                         <  2:349478/140‑1 (MQ=255)           taaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACg                                                                                                                       >  1:198223/1‑140 (MQ=255)                     ggTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCt                                                                                                             >  1:445975/1‑140 (MQ=255)                      gTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTg                                                                                                            >  1:180365/1‑140 (MQ=255)                            aCGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgttg                                                                                                                    <  2:489970/126‑1 (MQ=255)                            aCGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgttg                                                                                                                    >  1:489970/1‑126 (MQ=255)                            aCGTTGAGGTTGGTCACCCAGCCACCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGAt                                                                                                      <  2:86818/140‑1 (MQ=255)                              gTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGAtct                                                                                                    >  2:232558/1‑140 (MQ=255)                                tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATctct                                                                                                  <  2:336373/140‑1 (MQ=255)                                 gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCtt                                                                                                 >  2:403858/1‑140 (MQ=255)                                 gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCtt                                                                                                 >  1:46231/1‑140 (MQ=255)                                 gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTACCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCtt                                                                                                 >  2:485694/1‑140 (MQ=255)                                       ggTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCgag                                                                                           >  2:432550/1‑140 (MQ=255)                                       ggTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCgag                                                                                           >  2:259348/1‑140 (MQ=255)                                           aCCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACAt                                                                                       <  1:407239/140‑1 (MQ=255)                                               aGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGcc                                                                                   >  1:496804/1‑140 (MQ=255)                                                gCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCt                                                                                  <  2:46231/140‑1 (MQ=255)                                                  cATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGc                                                                                >  2:454958/1‑140 (MQ=255)                                                             gcgcgGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACg                                                                     >  2:357740/1‑140 (MQ=255)                                                              cgcgGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACgg                                                                    >  1:14640/1‑140 (MQ=255)                                                              cgcgGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACgg                                                                    >  1:408525/1‑140 (MQ=255)                                                                     cACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGTGAGACATCGCCTGCACACCGAGACGGTTacgac                                                             <  1:403858/140‑1 (MQ=255)                                                                        cGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTc                                                          >  2:308671/1‑140 (MQ=255)                                                                             gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcc                                                     <  1:308671/140‑1 (MQ=255)                                                                                 aaGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcctcct                                                 <  1:357740/140‑1 (MQ=255)                                                                                      ccAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGt                                            >  1:73954/1‑140 (MQ=255)                                                                                      ccAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGt                                            <  2:180365/140‑1 (MQ=255)                                                                                       cAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGtt                                           <  1:454958/140‑1 (MQ=255)                                                                                         gTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTtc                                         >  2:1024/1‑140 (MQ=255)                                                                                           tttGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTC‑‑CGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCt                                     >  1:12585/1‑140 (MQ=255)                                                                                             tGCCAGATTGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCt                                     >  1:371414/1‑140 (MQ=255)                                                                                               ccAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCtgt                                   >  1:450742/1‑140 (MQ=255)                                                                                                 aGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGTGTTTCTCCTGTGc                                 <  2:445975/140‑1 (MQ=255)                                                                                                   aTCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCa                               <  2:450742/140‑1 (MQ=255)                                                                                                       cgGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGTCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTa                            >  2:510183/1‑139 (MQ=255)                                                                                                       cgGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGTCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTa                            <  1:510183/139‑1 (MQ=255)                                                                                                              ccTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGataa                    <  1:315515/140‑1 (MQ=255)                                                                                                                   gcTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGc               >  1:436167/1‑140 (MQ=255)                                                                                                                       tCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACa           <  1:305380/140‑1 (MQ=255)                                                                                                                        cGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACAt          >  2:335678/1‑140 (MQ=255)                                                                                                                            aaCTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGc      >  1:160321/1‑140 (MQ=255)                                                                                                                                gATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTgc  <  2:496804/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGCTTGC  >  NC_000913/3388591‑3388857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑