Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,670	C→A	T1160K (ACG→AAG)	rhsB →	rhs element protein RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,670	0	C	A	100.0%	110.5 / NA	42	T1160K (ACG→AAG)	rhsB	rhs element protein RhsB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/19);  total (23/19)

AGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCC  >  NC_000913/3622534‑3622795                                                                                                                                         |                                                                                                                              aGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGa                                                                                                                            <  2:529158/140‑1 (MQ=17)    cgccgcTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCaaggaagg                                                                                                                         <  2:419838/140‑1 (MQ=17)      ccgcTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGACCTGCCGCTGGCGCTCATCAGCAAGGAAggga                                                                                                                       <  1:244148/140‑2 (MQ=9)              tggcATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCg                                                                                                               >  2:427012/1‑140 (MQ=14)              tggcATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCg                                                                                                               <  1:99667/140‑1 (MQ=9)                gcATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCgtg                                                                                                             <  2:118423/140‑1 (MQ=12)                   tCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCgtggtg                                                                                                          <  2:64178/140‑1 (MQ=12)                     gTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCCCCTGTACCACTTCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcg                                                                                                        <  2:375773/140‑1 (MQ=255)                      tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:546525/1‑140 (MQ=18)                      tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       <  1:73926/140‑1 (MQ=12)                      tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:97289/1‑140 (MQ=18)                      tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       <  1:419266/140‑1 (MQ=12)                      tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:249915/1‑140 (MQ=18)                      tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:23360/1‑140 (MQ=18)                         ggCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGa                                                                                                    <  1:427012/140‑1 (MQ=12)                               aCCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATatga                                                                                              >  1:241052/1‑140 (MQ=18)                                  gTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATAtgatga                                                                                           <  2:463581/140‑1 (MQ=12)                                  gTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATAtgatga                                                                                           <  1:487247/140‑1 (MQ=12)                                             gCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAcc                                                                                >  2:341809/1‑140 (MQ=14)                                             gCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAcc                                                                                >  1:119932/1‑140 (MQ=14)                                                        tGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCtgaatgaa                                                                     >  1:189796/1‑140 (MQ=18)                                                           aCCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAagag                                                                  >  2:102030/1‑140 (MQ=21)                                                                gtgtACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAAccc                                                             >  2:190062/1‑140 (MQ=21)                                                                     cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  2:315179/1‑140 (MQ=21)                                                                     cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  2:3954/1‑140 (MQ=21)                                                                     cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  1:509349/1‑140 (MQ=21)                                                                     cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  2:533233/1‑140 (MQ=21)                                                                           ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                  >  2:7126/1‑140 (MQ=255)                                                                           ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                  >  2:160630/1‑140 (MQ=255)                                                                           ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                  >  1:433826/1‑140 (MQ=255)                                                                               cgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcagca                                              <  2:302804/140‑1 (MQ=255)                                                                                            tGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTg                                 >  2:335503/1‑140 (MQ=255)                                                                                                  aCTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCgggg                           <  2:249915/140‑1 (MQ=255)                                                                                                  aCTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCgggg                           <  2:119932/140‑1 (MQ=255)                                                                                                      cGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGgcagc                       >  2:453998/1‑140 (MQ=255)                                                                                                        aCCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGcagcag                     >  2:390512/1‑140 (MQ=255)                                                                                                        aCCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGcagcag                     >  1:181595/1‑140 (MQ=255)                                                                                                        aCCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGcagcag                     >  1:425774/1‑140 (MQ=11)                                                                                                                ggCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTAtgatga             <  2:189796/140‑1 (MQ=255)                                                                                                                          tGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc   <  1:533233/140‑1 (MQ=255)                                                                                                                          tGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc   <  2:509349/140‑1 (MQ=255)                                                                                                                          tGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc   <  1:3954/140‑1 (MQ=255)                                                                                                                           ggCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGcc  <  1:437816/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                              AGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCC  >  NC_000913/3622534‑3622795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑