Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,678	G→A	A1163T (GCA→ACA)	rhsB →	rhs element protein RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,678	0	G	A	100.0%	105.6 / NA	40	A1163T (GCA→ACA)	rhsB	rhs element protein RhsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/17);  total (23/17)

TGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCC  >  NC_000913/3622547‑3622795                                                                                                                                    |                                                                                                                      tggcATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCg                                                                                                               <  1:99667/140‑1 (MQ=9) tggcATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCg                                                                                                               >  2:427012/1‑140 (MQ=14)   gcATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCgtg                                                                                                             <  2:118423/140‑1 (MQ=12)      tCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCgtggtg                                                                                                          <  2:64178/140‑1 (MQ=12)        gTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCCCCTGTACCACTTCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcg                                                                                                        <  2:375773/140‑1 (MQ=255)         tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       <  1:73926/140‑1 (MQ=12)         tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:546525/1‑140 (MQ=18)         tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:97289/1‑140 (MQ=18)         tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       <  1:419266/140‑1 (MQ=12)         tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:249915/1‑140 (MQ=18)         tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTgcgc                                                                                                       >  1:23360/1‑140 (MQ=18)            ggCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGa                                                                                                    <  1:427012/140‑1 (MQ=12)                  aCCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATatga                                                                                              >  1:241052/1‑140 (MQ=18)                     gTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATAtgatga                                                                                           <  2:463581/140‑1 (MQ=12)                     gTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATAtgatga                                                                                           <  1:487247/140‑1 (MQ=12)                                gCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAcc                                                                                >  2:341809/1‑140 (MQ=14)                                gCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAcc                                                                                >  1:119932/1‑140 (MQ=14)                                           tGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCtgaatgaa                                                                     >  1:189796/1‑140 (MQ=18)                                              aCCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAagag                                                                  >  2:102030/1‑140 (MQ=21)                                                   gtgtACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAAccc                                                             >  2:190062/1‑140 (MQ=21)                                                        cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  2:3954/1‑140 (MQ=21)                                                        cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  1:509349/1‑140 (MQ=21)                                                        cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  2:533233/1‑140 (MQ=21)                                                        cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                        >  2:315179/1‑140 (MQ=21)                                                              ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                  >  2:7126/1‑140 (MQ=255)                                                              ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                  >  2:160630/1‑140 (MQ=255)                                                              ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                  >  1:433826/1‑140 (MQ=255)                                                                  cgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcagca                                              <  2:302804/140‑1 (MQ=255)                                                                               tGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTg                                 >  2:335503/1‑140 (MQ=255)                                                                                     aCTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCgggg                           <  2:249915/140‑1 (MQ=255)                                                                                     aCTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCgggg                           <  2:119932/140‑1 (MQ=255)                                                                                         cGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGgcagc                       >  2:453998/1‑140 (MQ=255)                                                                                           aCCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGcagcag                     >  2:390512/1‑140 (MQ=255)                                                                                           aCCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGcagcag                     >  1:181595/1‑140 (MQ=255)                                                                                           aCCATCGCGGCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACCTGCACCAGCCGTACCGTCTGCCAGGGcagcag                     >  1:425774/1‑140 (MQ=11)                                                                                                   ggCCTGCCGCTGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTAtgatga             <  2:189796/140‑1 (MQ=255)                                                                                                             tGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc   <  1:533233/140‑1 (MQ=255)                                                                                                             tGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc   <  2:509349/140‑1 (MQ=255)                                                                                                             tGGCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc   <  1:3954/140‑1 (MQ=255)                                                                                                              ggCGCTCATCAGCAAGGAAGGGACAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGcc  <  1:437816/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                      TGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCC  >  NC_000913/3622547‑3622795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑