Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,649,339	T→C	T135T (ACT→ACC)	arsB →	arsenite/antimonite:H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,649,339	0	T	C	100.0%	88.4 / NA	27	T135T (ACT→ACC)	arsB	arsenite/antimonite:H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

CGTGGTCGCTTGCTGTTTACCTGGATTGTCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACTACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCTCGGTGAT  >  NC_000913/3649202‑3649470                                                                                                                                          |                                                                                                                                    cgtggtcgCTTGCTGTTTACCTGGATTGTCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGcaccac                                                                                                                                   >  1:316836/1‑140 (MQ=255) cgtggtcgCTTGCTGTTTACCTGGATTGTCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGcaccac                                                                                                                                   >  1:362233/1‑140 (MQ=255)             cTGTTTACCTGGATTGTCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGt                                                                                                                       <  1:335351/140‑1 (MQ=255)                        gATTGTCCTGCTCGGTGCTGTCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCg                                                                                                            >  1:122952/1‑140 (MQ=255)                         aTTGTCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCgg                                                                                                           <  2:219535/140‑1 (MQ=255)                          ttGTCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGa                                                                                                          >  1:147008/1‑140 (MQ=255)                             tCCTGCTCGGTGCTGCCGTTGGCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTc                                                                                                       >  2:386092/1‑140 (MQ=255)                             tCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTc                                                                                                       >  2:52095/1‑140 (MQ=255)                             tCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTc                                                                                                       >  2:118236/1‑140 (MQ=255)                                tGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCAtt                                                                                                    <  2:316836/140‑1 (MQ=255)                                       tgctgcCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATa                                                                                             >  1:330068/1‑140 (MQ=255)                                               ttGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGc                                                                                     <  1:386092/140‑1 (MQ=255)                                                 gccgccCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCt                                                                                   >  2:4358/1‑140 (MQ=255)                                                 gccgccCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCt                                                                                   >  1:120163/1‑140 (MQ=255)                                                 gccgccCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCt                                                                                   >  2:331566/1‑140 (MQ=255)                                                      ccTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGc                                                                              <  2:496174/140‑1 (MQ=255)                                                               cAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGtct                                                                     <  1:512979/140‑1 (MQ=255)                                                                aaTGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGtctc                                                                    <  1:4358/140‑1 (MQ=255)                                                                    atgGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTTAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAAc                                                                <  1:436085/140‑1 (MQ=255)                                                                    atgGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAAc                                                                <  2:367094/140‑1 (MQ=255)                                                                          cggcgcTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTg                                                          <  2:120163/140‑1 (MQ=255)                                                                                        acacCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGc                                            >  2:86985/1‑140 (MQ=255)                                                                                           ccGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGa                                         <  1:52095/140‑1 (MQ=255)                                                                                             gATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGAtt                                       >  1:2783/1‑140 (MQ=255)                                                                                                                      ttAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATa              <  1:331566/140‑1 (MQ=255)                                                                                                                      ttAGGGTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATa              <  1:201944/140‑1 (MQ=255)                                                                                                                          ggTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGcc          >  1:194523/1‑140 (MQ=255)                                                                                                                           gTTCAGTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCt         <  2:362233/140‑1 (MQ=255)                                                                                                                                gTAAAGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATGCCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCTCGGTg    <  2:230617/140‑1 (MQ=255)                                                                                                                                  aaaGGCACCACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCTCGGTGAt  <  2:2783/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CGTGGTCGCTTGCTGTTTACCTGGATTGTCCTGCTCGGTGCTGCCGTTGCCGCCCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACACCGATTGTCATCGCCATGCTGCTGGCTTTAGGGTTCAGTAAAGGCACTACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTGAATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCTCGGTGAT  >  NC_000913/3649202‑3649470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑