Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,271	(T)8→7	intergenic (+41/‑112)	cof → / → decR	HMP‑PP phosphatase/DNA‑binding transcriptional activator DecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,264	0	T	.	100.0%	325.2 / NA	77	intergenic (+34/‑119)	cof/decR	HMP‑PP phosphatase/DNA‑binding transcriptional activator DecR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (34/43);  total (34/43)

CCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGA  >  NC_000913/468132‑468399                                                                                                                                     |                                                                                                                                        ccGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATC‑‑CATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGtttttttgcg                                                                                                                                <  2:94308/140‑4 (MQ=255)  cGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGtttttttgc                                                                                                                                 <  1:1320590/140‑3 (MQ=255)   gCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGtttttttgcg                                                                                                                                <  2:969152/140‑4 (MQ=255)     aTTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGtt                                                                                                                             <  2:807386/140‑1 (MQ=255)      tttACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTg                                                                                                                            >  2:561260/1‑140 (MQ=255)       ttACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGa                                                                                                                           <  1:673405/140‑1 (MQ=255)       ttACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGa                                                                                                                           <  1:38878/140‑1 (MQ=255)         aCCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAAt                                                                                                                         <  2:470892/140‑1 (MQ=255)                aTTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCAt                                                                                                                  >  2:1476325/1‑140 (MQ=255)                aTTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCAt                                                                                                                  >  1:333987/1‑140 (MQ=255)                aTTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCAt                                                                                                                  >  2:1393214/1‑140 (MQ=255)                      cATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTtgtg                                                                                                            <  1:1444576/140‑1 (MQ=255)                      cATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTtgtg                                                                                                            <  1:1476325/140‑1 (MQ=255)                      cATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTtgtg                                                                                                            <  2:450842/140‑1 (MQ=255)                      cATTGCCGAAATCAGGCTGTCGCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTtgtg                                                                                                            <  1:1543127/140‑1 (MQ=255)                        ttGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGcc                                                                                                          >  2:1184479/1‑140 (MQ=255)                         tGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGcc                                                                                                          >  2:824281/1‑139 (MQ=255)                         tGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGcc                                                                                                          <  1:824281/139‑1 (MQ=255)                          gCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAATGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgt                                                                                                        >  2:699940/1‑140 (MQ=255)                          gCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgt                                                                                                        >  1:212814/1‑140 (MQ=255)                          gCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgt                                                                                                        <  2:333987/140‑1 (MQ=255)                          gCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgt                                                                                                        <  2:571129/140‑1 (MQ=255)                          gCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgt                                                                                                        <  1:1393214/140‑1 (MQ=255)                          gCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTCGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgt                                                                                                        >  1:341726/1‑140 (MQ=255)                           ccGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAATGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgtg                                                                                                       <  1:699940/140‑1 (MQ=255)                            cGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCgtgg                                                                                                      <  2:1392405/140‑1 (MQ=255)                               aaTCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGgtgt                                                                                                   <  1:1184479/140‑1 (MQ=255)                                   aGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTaa                                                                                               >  1:436360/1‑140 (MQ=255)                                       tGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCg                                                                                           <  1:1387019/140‑1 (MQ=255)                                        gTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGc                                                                                          <  2:212814/140‑1 (MQ=255)                                        gTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGc                                                                                          <  2:1463012/140‑1 (MQ=255)                                         tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTTTGCCGTGGTGTTTAAACCGca                                                                                         <  2:1153050/140‑1 (MQ=255)                                         tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGca                                                                                         <  2:436360/140‑1 (MQ=255)                                         tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGca                                                                                         <  1:1019891/140‑1 (MQ=255)                                         tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGca                                                                                         <  2:523825/140‑1 (MQ=255)                                         tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGca                                                                                         <  2:1222408/140‑1 (MQ=255)                                         tctctcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGca                                                                                         <  2:619046/140‑1 (MQ=255)                                             tcACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGa                                                                                     <  2:130041/140‑1 (MQ=255)                                                    ttGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAAtt                                                                              <  2:1565570/140‑1 (MQ=255)                                                         gCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGt                                                                         >  2:285392/1‑140 (MQ=255)                                                              ggctggACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGAttt                                                                    <  1:561260/140‑1 (MQ=255)                                                                    aCTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCtt                                                              >  1:328388/1‑140 (MQ=255)                                                                       aTCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaa                                                           >  2:651713/1‑140 (MQ=255)                                                                       aTCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaa                                                           >  2:208064/1‑140 (MQ=255)                                                                        tCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCg                                                                          <  1:1374467/124‑1 (MQ=255)                                                                        tCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCg                                                                          >  2:1374467/1‑124 (MQ=255)                                                                        tCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaaa                                                          <  1:285392/140‑1 (MQ=255)                                                                         ccACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaaaa                                                         >  2:1063505/1‑140 (MQ=255)                                                                             aTCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaaaataaa                                                     >  1:875505/1‑140 (MQ=255)                                                                             aTCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaaaataaa                                                     >  1:829919/1‑140 (MQ=255)                                                                             aTCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTtaaaataaa                                                     >  2:542039/1‑140 (MQ=255)                                                                                    ttATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTaaaa                                              >  2:161892/1‑140 (MQ=255)                                                                                      aTTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAaga                                            <  2:829919/140‑1 (MQ=255)                                                                                       ttCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAagag                                           >  1:1009612/1‑140 (MQ=255)                                                                                          ccccGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGaaa                                        >  1:1526110/1‑140 (MQ=255)                                                                                             cGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAaga                                            >  2:283052/1‑133 (MQ=255)                                                                                             cGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAaga                                            <  1:283052/133‑1 (MQ=255)                                                                                               aaTAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAAt                                   >  1:265486/1‑140 (MQ=255)                                                                                                     gagaTCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCtg                             <  1:1063505/140‑1 (MQ=255)                                                                                                        aTCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTgg                          <  2:328388/140‑1 (MQ=255)                                                                                                        aTCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTgg                          <  1:1117119/140‑1 (MQ=255)                                                                                                         tCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGa                         <  1:161892/140‑1 (MQ=255)                                                                                                         tCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGa                         <  2:1009612/140‑1 (MQ=255)                                                                                                         tCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGa                         <  2:927849/140‑1 (MQ=255)                                                                                                         tCCCTTCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGa                         <  2:611565/140‑1 (MQ=255)                                                                                                             ttCCAGCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAggg                     <  1:208064/140‑1 (MQ=255)                                                                                                                  gCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATg                >  2:1017435/1‑140 (MQ=255)                                                                                                                  gCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATg                <  2:265486/140‑1 (MQ=255)                                                                                                                  gCACCGGGCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCCGTGGAAGGGCTATg                <  2:1526110/140‑1 (MQ=255)                                                                                                                       gggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGa           >  2:1425018/1‑140 (MQ=255)                                                                                                                        ggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGAt          >  1:702117/1‑140 (MQ=255)                                                                                                                        ggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGAt          >  1:256851/1‑140 (MQ=255)                                                                                                                        ggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGAt          >  2:335680/1‑140 (MQ=255)                                                                                                                        ggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGAt          >  1:970847/1‑140 (MQ=255)                                                                                                                        ggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGAt          >  1:700244/1‑140 (MQ=255)                                                                                                                        ggCAATTGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGAt          >  1:106237/1‑140 (MQ=255)                                                                                                                             ttGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt     <  1:1425018/140‑1 (MQ=255)                                                                                                                             ttGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt     >  2:900693/1‑140 (MQ=255)                                                                                                                             ttGCCCGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt     <  1:335680/140‑1 (MQ=255)                                                                                                                                cccGG‑TTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGa  >  2:105893/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGA  >  NC_000913/468132‑468399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑