Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	216,145	(C)6→7	intergenic (+166/+34)	nlpE → / ← yaeF	lipoprotein NlpE/peptidase C92 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,139	1	.	C	100.0%	265.9 / NA	75	intergenic (+160/+40)	nlpE/yaeF	lipoprotein NlpE/peptidase C92 family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (32/43);  total (32/43)

CGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑CCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCA  >  NC_000913/216002‑216272                                                                                                                                             |                                                                                                                                      cGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑cc                                                                                                                                     <  2:770490/140‑1 (MQ=255) cGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑cc                                                                                                                                     <  1:1235308/140‑1 (MQ=255)  gCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccc                                                                                                                                    <  1:300658/140‑1 (MQ=255)   cAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑cccc                                                                                                                                   <  1:256064/140‑1 (MQ=255)      aaCAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccccccc                                                                                                                                >  1:1305749/1‑139 (MQ=255)         aGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccccccctca                                                                                                                             <  2:1305749/140‑5 (MQ=255)         aGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccccccctca                                                                                                                             <  1:60297/140‑5 (MQ=255)           gCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATa                                                                                                                            >  2:727885/1‑140 (MQ=255)           gCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATa                                                                                                                            >  1:1307908/1‑140 (MQ=255)           gCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccccccctca                                                                                                                             <  1:1312900/138‑5 (MQ=255)           gCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccccccctca                                                                                                                             >  2:1312900/1‑134 (MQ=255)                aaGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTa                                                                                                                       >  1:1233509/1‑140 (MQ=255)                 aGGATATATTTCAACATTTTGAATTTGCACG‑TTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAg                                                                                                                     <  2:490062/140‑1 (MQ=255)                  ggATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccc                                                                                                                                    >  2:672143/1‑124 (MQ=255)                  ggATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccc                                                                                                                                    <  1:672143/124‑1 (MQ=255)                    atatatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGc                                                                                                                   <  1:627460/140‑1 (MQ=255)                     tatatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAg                                                                                                                     <  2:1535227/137‑1 (MQ=255)                     tatatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAg                                                                                                                     >  1:1535227/1‑137 (MQ=255)                     tatatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCg                                                                                                                  >  2:105398/1‑140 (MQ=255)                        atTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGAt                                                                                                               <  2:33583/140‑1 (MQ=255)                          ttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATaa                                                                                                             >  1:309084/1‑140 (MQ=255)                          ttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccccccctca                                                                                                                             <  2:311529/123‑5 (MQ=255)                          ttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑ccccccctca                                                                                                                             >  1:311529/1‑119 (MQ=255)                          ttCAACATTTTGAATTTGCACGTTCTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATaa                                                                                                             >  1:566286/1‑140 (MQ=255)                             aaCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGc                                                                                                          >  1:107198/1‑140 (MQ=255)                              aCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCa                                                                                                         >  1:142977/1‑140 (MQ=255)                              aCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCa                                                                                                         <  2:115761/140‑1 (MQ=255)                              aCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCa                                                                                                         <  1:1313180/140‑1 (MQ=255)                              aCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCa                                                                                                         >  2:750915/1‑140 (MQ=255)                                aTTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCAtt                                                                                                       <  1:433794/140‑1 (MQ=255)                                aTTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCAtt                                                                                                       <  2:107198/140‑1 (MQ=255)                                  tttGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAg                                                                                                            >  2:51650/1‑133 (MQ=255)                                  tttGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAg                                                                                                            >  2:275843/1‑133 (MQ=255)                                  tttGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAg                                                                                                            <  1:275843/133‑1 (MQ=255)                                  tttGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAg                                                                                                            <  1:51650/133‑1 (MQ=255)                                  tttGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTa                                                                                                     >  1:910894/1‑140 (MQ=255)                                           gCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                                                                            <  2:910894/140‑1 (MQ=255)                                           gCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                                                                            <  2:985164/140‑1 (MQ=255)                                           gCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                                                                            <  2:1274127/140‑1 (MQ=255)                                            cACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAcc                                                                                           >  1:508322/1‑140 (MQ=255)                                              cGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCgg                                                                                         <  2:199848/140‑1 (MQ=255)                                               ccgagccgTAGGCCGGATAAGGCGTTCACGCTGCATCCGGCA‑‑‑‑‑‑CCCGGAGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAAc                                                                                  >  2:1226777/8‑140 (MQ=11)                                                ttttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGt                                                                                       <  2:829909/140‑1 (MQ=255)                                                   catGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCCGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCaa                                                                                    <  2:1416743/138‑1 (MQ=255)                                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaac                                                                              >  1:219477/1‑140 (MQ=255)                                                          gCCGAATAAGGCGTTCACGCCGCATCCGGCAA‑‑‑‑‑‑TCAACGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGa                                                                       >  2:114773/1‑140 (MQ=17)                                                           ccGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaacaa                                                                            >  1:86283/1‑140 (MQ=255)                                                                 aaGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGAc                                                                      <  2:309084/140‑1 (MQ=255)                                                                 aaGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGAc                                                                      <  1:474990/140‑1 (MQ=255)                                                                  aGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGAcc                                                                     <  1:727885/140‑1 (MQ=255)                                                                  aGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGAcc                                                                     <  2:1407125/140‑1 (MQ=255)                                                                    gCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaa                                                                               <  1:145064/128‑1 (MQ=255)                                                                    gCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaa                                                                               >  2:145064/1‑128 (MQ=255)                                                                      gTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGcc                                                                 <  1:750915/140‑1 (MQ=255)                                                                      gTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGcc                                                                 <  2:566286/140‑1 (MQ=255)                                                                      gTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGcc                                                                 <  1:1231074/140‑1 (MQ=255)                                                                       tttACGCCGCATCGGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCt                                                                <  2:1056394/140‑1 (MQ=255)                                                                         tACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGAc                                                                      >  1:1520852/1‑132 (MQ=255)                                                                         tACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGAc                                                                      <  2:1520852/132‑1 (MQ=255)                                                                         tACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTg                                                              >  1:958491/1‑140 (MQ=255)                                                                                  tCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATc                                                     >  1:1556237/1‑140 (MQ=255)                                                                                   ccGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATcc                                                    <  2:109456/140‑1 (MQ=255)                                                                                               cTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGt                                        <  1:105398/140‑1 (MQ=255)                                                                                               cTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGt                                        <  1:1374520/140‑1 (MQ=255)                                                                                               cTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGt                                        <  2:1307908/140‑1 (MQ=255)                                                                                                 cAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTCCAGGTGc                                      <  2:1556237/140‑1 (MQ=255)                                                                                                    cGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGcc                                     >  2:831074/1‑138 (MQ=255)                                                                                                    cGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGcc                                     <  1:831074/138‑1 (MQ=255)                                                                                                    cGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGc                                      >  2:248974/1‑137 (MQ=255)                                                                                                    cGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGc                                      <  1:248974/137‑1 (MQ=255)                                                                                                     gCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCaa                                  <  2:958491/140‑1 (MQ=255)                                                                                                       cTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                <  1:1392660/140‑1 (MQ=255)                                                                                                       cTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                <  1:1226777/140‑1 (MQ=255)                                                                                                       cTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                >  1:981833/1‑140 (MQ=255)                                                                                                       cTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                <  2:1233509/140‑1 (MQ=255)                                                                                                         gATGCGACGCTTAACGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCaa                                  <  2:324900/137‑1 (MQ=39)                                                                                                            gCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                >  2:1617434/1‑135 (MQ=255)                                                                                                            gCGACGC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                <  1:1617434/135‑1 (MQ=255)                                                                                                                 gC‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACt                      >  1:581731/1‑140 (MQ=255)                                                                                                                  c‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                >  1:897149/1‑129 (MQ=255)                                                                                                                  c‑TGGCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACa                                <  2:897149/129‑1 (MQ=255)                                                                                                                     ggCGCGTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCg                   >  2:1343642/1‑140 (MQ=255)                                                                                                                          gTCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCt              <  2:142977/140‑1 (MQ=255)                                                                                                                           tCTTATCATGCCTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTg             >  2:914137/1‑140 (MQ=255)                                                                                                                              tATCATGCCTACACC‑CCCCCCTCATACCTTAAGGCGGATAAGGCATTTCCTTTATCCCCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCt          <  2:810302/140‑1 (MQ=255)                                                                                                                                     ccTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGc   >  1:569805/1‑140 (MQ=255)                                                                                                                                     ccTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGc   <  2:569805/140‑1 (MQ=255)                                                                                                                                      cTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCa  <  1:914137/140‑1 (MQ=255)                                                                                                                                      cTACA‑ACCCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCa  >  2:1071182/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                      CGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGC‑TGGCGCGTCTTATCATGCCTACA‑A‑CCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATATAAATCCCAGGCTTCAGGTGCCCAACATACTGTAACTGCGTTTCTGGCTTAAACGCA  >  NC_000913/216002‑216272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑