Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,218	(G)7→8	intergenic (+389/+347)	ycdU → / ← serX	uncharacterized protein YcdU/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,211	1	.	G	100.0%	11.4 / NA	6	intergenic (+382/+354)	ycdU/serX	uncharacterized protein YcdU/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (0/6);  total (0/6)

GCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCC  >  NC_000913/1097078‑1097289                                                                                                                                        |                                                                               gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGA‑gggggg                                                                          >  2:418927/1‑140 (MQ=35)    ttCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGA‑gggggggga                                                                       <  1:171778/140‑3 (MQ=21)                        gtGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCAGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGAGGGGGGGGATTGACTCGCTTCGCTCGccc                                                    <  2:1371221/139‑1 (MQ=255)                                            ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGTAATACACGAAAAAAAAGCCCGTACCTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTg                                <  1:175602/140‑1 (MQ=255)                                                    ggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg                        <  1:418927/140‑1 (MQ=17)                                                    ggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg                        <  2:1305071/140‑1 (MQ=17)                                                        gctcggccccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCt                    <  2:74282/134‑1 (MQ=255)                                                                   cGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGGG‑GGGGGGGGTTGGCGCGCTTCGCTCCCCCTCCGGGCAGCCCCCCCCCCGCGGTCCCCGTCTGTCCCAcccgc         >  2:1510436/1‑136 (MQ=2)                                                                          tgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGcc  <  2:1116641/140‑1 (MQ=17)                                                                                                                                        |                                                                               GCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCC  >  NC_000913/1097078‑1097289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑