Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,215,832	(C)5→6	coding (645/732 nt)	yehW ←	glycine betaine ABC transporter membrane subunit YehW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,215,827	1	.	C	100.0%	203.4 / NA	58	A217G (GCG→GGG)	yehW	glycine betaine ABC transporter membrane subunit YehW
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/35);  total (23/35)

TGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACG‑CCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGC  >  NC_000913/2215688‑2215960                                                                                                                                             |                                                                                                                                      tgttcctgttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACg                                                                                                                                        <  2:1211369/140‑1 (MQ=255) tgttcctgttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACg                                                                                                                                        <  1:678417/140‑1 (MQ=255) tgttcctgttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACg                                                                                                                                        <  2:1215765/140‑1 (MQ=255) tgttcctgttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACg                                                                                                                                        <  1:769536/140‑1 (MQ=255) tgttcctgttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACg                                                                                                                                        <  1:1282270/140‑1 (MQ=255)      ctgttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACg                                                                                                                                        >  1:875024/1‑135 (MQ=255)      ctgttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACg                                                                                                                                        <  2:875024/135‑1 (MQ=255)        gttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACG‑cccccct                                                                                                                                <  1:232252/140‑3 (MQ=255)        gttTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACG‑cccccct                                                                                                                                <  2:1008939/140‑3 (MQ=255)         tttATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTCATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACG‑cccccctg                                                                                                                               >  1:386370/1‑137 (MQ=255)            aTGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGAt                                                                                                                             <  1:769672/140‑1 (MQ=255)             tGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATc                                                                                                                            >  1:1225158/1‑140 (MQ=255)                cGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACG‑cccccc                                                                                                                                 <  1:1457618/131‑2 (MQ=255)                cGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACG‑cccccc                                                                                                                                 >  2:1457618/1‑130 (MQ=255)                   tAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATAc                                                                                                                      >  1:1468042/1‑140 (MQ=255)                    aGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATAcg                                                                                                                     <  2:386370/140‑1 (MQ=255)                    aGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATAcg                                                                                                                     <  1:1100793/140‑1 (MQ=255)                     ggCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATAcgc                                                                                                                    >  1:189516/1‑140 (MQ=255)                     ggCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATAcgc                                                                                                                    <  2:1371287/140‑1 (MQ=255)                               gtggCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAAt                                                                                                          >  2:90056/1‑140 (MQ=255)                                   cGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGc                                                                                                      <  2:817612/140‑1 (MQ=255)                                    gTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCt                                                                                                     <  2:595467/140‑1 (MQ=255)                                    gTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCt                                                                                                     <  2:823691/140‑1 (MQ=255)                                    gTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCt                                                                                                     <  2:1225158/140‑1 (MQ=255)                                        tGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTa                                                                                                                <  2:505168/125‑1 (MQ=255)                                        tGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTa                                                                                                                >  1:505168/1‑125 (MQ=255)                                             gCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCgat                                                                                            <  2:374396/140‑1 (MQ=255)                                                ggTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCAC‑‑ACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                       <  1:688340/140‑1 (MQ=255)                                                  ttATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                       <  2:230714/140‑1 (MQ=255)                                                  ttATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                       <  2:608059/140‑1 (MQ=255)                                                      aCCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATggg                                                                                   <  1:1219861/140‑1 (MQ=255)                                                         tttATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGt                                                                                <  2:1614361/140‑1 (MQ=255)                                                           tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGcc                                                                                                <  1:310218/122‑1 (MQ=255)                                                           tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGcc                                                                                                >  2:310218/1‑122 (MQ=255)                                                           tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTAc                                                                              >  1:1096194/1‑140 (MQ=255)                                                           tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTAc                                                                              >  2:978558/1‑140 (MQ=255)                                                              tttGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACACACGCGGTATTAAATCCGTTAAGCCCGATGATGATGGGCGTACCCa                                                                           <  2:1518037/140‑1 (MQ=255)                                                                tGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGc                                                                         >  1:23805/1‑140 (MQ=255)                                                                  cGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGt                                                                       >  2:647828/1‑140 (MQ=255)                                                                   gTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTg                                                                      <  1:1600716/140‑1 (MQ=255)                                                                   gTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTg                                                                      >  1:507063/1‑140 (MQ=255)                                                                           tAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGcccc                                                              <  1:778445/140‑1 (MQ=255)                                                                                 ccTGCACCAGCCTTTCAAGCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCt                                                                    <  1:176702/128‑1 (MQ=255)                                                                                 ccTGCACCAGCCTTTCAAGCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCt                                                                    >  2:176702/1‑128 (MQ=255)                                                                                   tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCt                                                                    >  2:54277/1‑126 (MQ=255)                                                                                   tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCt                                                                    <  1:54277/126‑1 (MQ=255)                                                                                   tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTg                                                      >  2:149924/1‑140 (MQ=255)                                                                                   tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTg                                                      >  1:618790/1‑140 (MQ=255)                                                                                   tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTg                                                      >  1:1175283/1‑140 (MQ=255)                                                                                          gCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAt                                               <  2:1468042/140‑1 (MQ=255)                                                                                          gCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAt                                               <  1:1490332/140‑1 (MQ=255)                                                                                           ccTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtc                                              >  1:982910/1‑140 (MQ=255)                                                                                               tCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtcgtcg                                          >  1:742171/1‑140 (MQ=255)                                                                                               tCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtcgtcg                                          <  1:978558/140‑1 (MQ=255)                                                                                               tCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtcgtcg                                          >  1:1362802/1‑140 (MQ=255)                                                                                                   aCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGt                                      <  1:1437639/140‑1 (MQ=255)                                                                                                    cAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTa                                     <  2:184623/140‑1 (MQ=255)                                                                                                    cAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTa                                     <  2:1096194/140‑1 (MQ=255)                                                                                                    cAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTa                                     <  2:52132/140‑1 (MQ=255)                                                                                                        cGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCaa                                 <  2:1174692/140‑1 (MQ=255)                                                                                                             tGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTg                            >  1:1520939/1‑140 (MQ=255)                                                                                                               cGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGAt                          <  2:618790/140‑1 (MQ=255)                                                                                                                     tCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACGTACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCaa                                 <  2:675332/127‑1 (MQ=255)                                                                                                                     tCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACGTACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCaa                                 >  1:675332/1‑127 (MQ=255)                                                                                                                     tCGCCGCCAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCAc                    >  2:439769/1‑140 (MQ=255)                                                                                                                       gccgccAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAAt                       >  2:1172754/1‑135 (MQ=255)                                                                                                                       gccgccAGTGCCACCAGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAAt                       <  1:1172754/135‑1 (MQ=255)                                                                                                                                  caccaGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCAcg       <  2:507063/140‑1 (MQ=255)                                                                                                                                  caccaGTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCAcg       <  2:1175283/140‑1 (MQ=255)                                                                                                                                       gTAACGCCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCAcgcccgc  <  2:23805/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                      TGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACG‑CCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGC  >  NC_000913/2215688‑2215960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑