| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,101,514 | 0 | G | T | 31.0% | 131.5 / 27.5 | 58 | intergenic (+84/‑176) | sodA/kdgT | superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base G (7/33); new base T (18/0); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAA > NC_000913/4101377‑4101648 | tGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTtgtg < 1:378373/140‑1 (MQ=255) tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAAtt > 1:15477/1‑140 (MQ=255) tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAAtt > 1:791495/1‑140 (MQ=255) aggAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTc < 1:49821/138‑1 (MQ=255) gggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATaa < 1:727933/140‑1 (MQ=255) aCGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTTTGATCAATTTC‑aaa > 1:1143685/1‑133 (MQ=255) aCGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑aaa < 2:1143685/133‑1 (MQ=255) aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGTCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAATAAAAACaa > 1:733289/1‑140 (MQ=255) aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAATAAAAACaa > 2:909060/1‑140 (MQ=255) aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAAATTT‑aaaaaaaaaaact > 2:49821/1‑137 (MQ=255) agcagcGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAAt < 2:17052/140‑1 (MQ=255) agcagcGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAAt < 1:659125/140‑1 (MQ=255) gcGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAATAAAAAAAATGATc > 1:260435/1‑140 (MQ=255) cGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATaaa < 2:108296/129‑1 (MQ=255) cGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATaaa > 1:108296/1‑129 (MQ=255) cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGat < 2:563190/140‑1 (MQ=255) gTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAATAAAAAAAAAGATCCGataaa > 2:31795/1‑140 (MQ=255) gAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAg < 1:909060/140‑1 (MQ=255) aaaaTAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGt < 2:15477/140‑1 (MQ=255) taatCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCa < 1:1563829/140‑1 (MQ=255) atCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAAt < 1:1177515/140‑1 (MQ=255) ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg < 1:31795/140‑1 (MQ=255) ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg < 2:518868/140‑1 (MQ=255) ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg < 2:171544/140‑1 (MQ=255) ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg < 2:746013/140‑1 (MQ=255) ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg < 1:1492140/140‑1 (MQ=255) tGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAATTTT‑AAAAAAAAAAAAAAGATCCGATAAAAATAAAAAAGCGGTTCAATTGAtgg > 2:1563829/1‑139 (MQ=255) gccgccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAATAAAAAAAATGATCCGATAAAAATAAAAAAACGTTTCAATTGAtgtg > 1:1142273/1‑140 (MQ=255) ccgccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTT‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTgg > 1:561098/1‑140 (MQ=255) ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTATTTTTGATCAATTTT‑AAAATAAAAAAAAAGATCCGGTAAAAATAAAAAAGCGTTTCAATTTATGTGGGTTTGAt > 2:1429249/1‑140 (MQ=255) gagacagggCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCa < 1:162362/133‑1 (MQ=255) cAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCAc < 2:912475/140‑1 (MQ=255) tGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTTTGATCAATTTT‑AAAATAAAAAAAAAAATCCCATAAAAATAAAAAAACGTTTCAATTTATTTGGGTTTTATCACttt > 2:943232/1‑140 (MQ=255) tGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAAAAAAAAAAAAAATTCGATAAAAAAAAAAAAACGGTTCAATTGATGTGGTTTTTATTACttt > 2:774348/1‑140 (MQ=255) gggCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa < 1:1557221/139‑1 (MQ=255) aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa < 1:959925/140‑1 (MQ=255) aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa < 1:945986/140‑1 (MQ=255) aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa < 2:1485083/140‑1 (MQ=255) cGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTGTGATCAATTTT‑AAAATAAAAACAAAGATCCGATAAAAATAAAACAGCGGTTCAATTGATGTGGTTTTTATCACTTTTAttt > 1:644804/1‑139 (MQ=255) tataGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAAATTT‑AAAATAAAAAAAATGATCCGGTAAAAATAAAAAAGCGTTTTAATTTATGTGGTTTTTATTACTTTTTTTGAt > 2:35217/1‑140 (MQ=255) ataGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGAtt < 1:832459/140‑1 (MQ=255) gCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAATAAAAACAATGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTGGTTTTGATCACTTTTTTTGATTAaat > 1:91709/1‑140 (MQ=255) gCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGGGGGTTTTTTTTGATCAATTTC‑AAAATAAAAACAAAGATCCGGTAAAAAAAAAAAAGCGTTTTAATTTATGGGGTTTTGATCACTTTTATTTATTaaaa > 2:959328/1‑139 (MQ=255) cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTT‑AAAAAAAAAAAAAAGATCCGATAAAAAAAAAAAAGCGTTTTAATTTATGTGGGTTTGATTACTTTTATTTATTAaatgc > 2:1557221/1‑139 (MQ=255) cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTC‑AAAAAAAAAACAAAGATCCGATAAAAAAAAAACAACGTTTCAATTGATGTGGTTTTGATTACTTttatttatctgtctc > 2:162362/1‑133 (MQ=255) cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTT‑AAAATAAAAAAAAAGATCCGATAAAAATAAAAAAACGTTTTAATTGATGTGGTTTTTATCACTTTTTTTTATTAaatgaat > 2:83122/1‑140 (MQ=255) cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatgaat < 2:597399/140‑1 (MQ=255) atatCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTC‑AAAATAAAAAAAATGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTGGTTTTGATCACTTTTTTTTATTAaatgaatg > 1:182368/1‑140 (MQ=255) cTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATTAATTTT‑AAAATAAAAAAAAAGATCCGAAAAAAAAAAAACAGCGTTTTAATTTATGTGGGTTTTATTACTTTTTTTTATTAAATTAATGTCTATCt > 2:1124223/1‑140 (MQ=255) aaaaTGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt < 1:35217/140‑1 (MQ=255) aaaaTGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt < 1:943232/140‑1 (MQ=255) aaaaTGAACCATCGCCAACGGCGGTGGGTTTTTTGTGATCAATTTT‑AAAAAAAAAAAAAAGATCCGATAAAAAAAAAAAAACGGTTTAATTTATGTGGGTTTGATCACTTTTATTGATTAAAAGAAAGTCTATCTTCGtt > 2:741801/1‑140 (MQ=255) gAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTT‑AAAATAAAAAAAATGATCCGATAAAAATAAAAAAGCGTTTCAATTTATGTGGGTTTGATTACTTTTATTGATTAAATGAATGTCTATCTTTGTTTCCAt > 2:881526/1‑140 (MQ=255) gAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCAt < 2:1326605/140‑1 (MQ=255) cATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCaa < 2:672771/140‑1 (MQ=255) tCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAacac < 2:91709/140‑1 (MQ=255) cggTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCa < 1:1429249/140‑1 (MQ=255) tggtTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTg < 1:959328/140‑1 (MQ=255) gtTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAg < 2:1142273/140‑1 (MQ=255) gtTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAg < 1:83122/140‑1 (MQ=255) ttttttTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGaa > 2:773235/1‑140 (MQ=255) | TGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTC‑AAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAA > NC_000913/4101377‑4101648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |