Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	30,005	A→G	T119A (ACG→GCG)	carA →	carbamoyl phosphate synthetase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	30,005	0	A	G	100.0%	349.8 / NA	97	T119A (ACG→GCG)	carA	carbamoyl phosphate synthetase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/55);  total (42/55)

TCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGACGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCA  >  NC_000913/29869‑30140                                                                                                                                         |                                                                                                                                        tcAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGgcgc                                                                                                                                      >  1:602289/1‑140 (MQ=255)    ggTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGtt                                                                                                                                   >  1:846850/1‑140 (MQ=255)             acaAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTCCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGcgcg                                                                                                                          >  2:1474923/1‑140 (MQ=255)               aaGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgag                                                                                                                        >  2:863611/1‑140 (MQ=255)               aaGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgag                                                                                                                        >  1:1266390/1‑140 (MQ=255)               aaGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgag                                                                                                                        >  1:619882/1‑140 (MQ=255)               aaGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgag                                                                                                                        >  1:166679/1‑140 (MQ=255)               aaGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgag                                                                                                                        >  1:4040/1‑140 (MQ=255)               aaGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCga                                                                                                                         >  1:785231/1‑139 (MQ=255)               aaGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCga                                                                                                                         <  2:785231/139‑1 (MQ=255)                aGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTTCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgaga                                                                                                                       >  2:1505183/1‑140 (MQ=255)                aGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGtt                                                                                                                                   >  1:109186/1‑128 (MQ=255)                aGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGtt                                                                                                                                   <  2:109186/128‑1 (MQ=255)                aGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgaga                                                                                                                       >  1:338553/1‑140 (MQ=255)                aGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCgaga                                                                                                                       <  2:539766/140‑1 (MQ=255)                    cTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAgg                                                                                                                   <  1:1427664/140‑1 (MQ=255)                     tGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGgc                                                                                                                  >  1:588661/1‑140 (MQ=255)                     tGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGgc                                                                                                                  >  1:675749/1‑140 (MQ=255)                      ggTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGgcg                                                                                                                 >  1:363025/1‑140 (MQ=255)                       gTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGgcgc                                                                                                                <  2:1266390/140‑1 (MQ=255)                       gTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGgcgc                                                                                                                <  1:1522963/140‑1 (MQ=255)                       gTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGgcgc                                                                                                                <  2:4040/140‑1 (MQ=255)                       gTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGgcgc                                                                                                                >  2:497259/1‑140 (MQ=255)                        tGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGca                                                                                                               <  2:619882/140‑1 (MQ=255)                        tGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGca                                                                                                               <  1:1474923/140‑1 (MQ=255)                        tGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGca                                                                                                               <  1:750825/140‑1 (MQ=255)                           ttCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAg                                                                                                            <  1:234312/140‑1 (MQ=255)                            tCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGa                                                                                                           >  2:736432/1‑140 (MQ=255)                            tCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGa                                                                                                           <  1:858857/140‑1 (MQ=255)                            tCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGa                                                                                                           <  1:1430642/140‑1 (MQ=255)                             cgcgACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGaa                                                                                                          >  1:829708/1‑140 (MQ=255)                             cgcgACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGaa                                                                                                          >  1:1107057/1‑140 (MQ=255)                             cgcgACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGaa                                                                                                          <  2:1119752/140‑1 (MQ=255)                             cgcgACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGaa                                                                                                          >  1:1626576/1‑140 (MQ=255)                             cgcgACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGaa                                                                                                          >  1:458746/1‑140 (MQ=255)                             cgcgACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGaa                                                                                                          >  1:1058791/1‑140 (MQ=255)                              gcgACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAAt                                                                                                         <  1:1505183/140‑1 (MQ=255)                                gACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATgg                                                                                                       <  2:481513/140‑1 (MQ=255)                                gACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATgg                                                                                                       <  1:1328665/140‑1 (MQ=255)                                gACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATgg                                                                                                       <  1:750183/140‑1 (MQ=255)                                             ttGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATcgcg                                                                                          <  1:497259/140‑1 (MQ=255)                                                             tAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATg                                                                          <  1:1157130/140‑1 (MQ=255)                                                                   cGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGgcgc                                                                    <  1:928025/140‑1 (MQ=255)                                                                     aaGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGGTGCGgcgc                                                                    >  2:1614066/1‑138 (MQ=255)                                                                     aaGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGGTGCGgcgc                                                                    <  1:1614066/138‑1 (MQ=255)                                                                     aaGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTg                                                                  <  2:883345/140‑1 (MQ=255)                                                                      aGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTgg                                                                 <  2:1626576/140‑1 (MQ=255)                                                                         ccTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGt                                                              <  2:338553/140‑1 (MQ=255)                                                                         ccTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGt                                                              <  2:633380/140‑1 (MQ=255)                                                                          ctctctTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGtt                                                             >  1:200716/1‑140 (MQ=255)                                                                          ctctctTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGtt                                                             >  2:1329807/1‑140 (MQ=255)                                                                            ctctTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAg                                                           >  2:1456878/1‑140 (MQ=255)                                                                                  ttACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAg                                                     >  2:213774/1‑140 (MQ=255)                                                                                  ttACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAg                                                     >  1:777476/1‑140 (MQ=255)                                                                                    aCCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGcc                                                   >  2:337794/1‑140 (MQ=255)                                                                                      cTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcg                                                 >  2:932701/1‑140 (MQ=255)                                                                                       tGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGt                                                              >  1:1529424/1‑126 (MQ=255)                                                                                       tGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGt                                                              <  2:1529424/126‑1 (MQ=255)                                                                                         aaaCGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgcgc                                              <  2:566771/140‑1 (MQ=255)                                                                                         aaaCGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgcgc                                              <  1:1456878/140‑1 (MQ=255)                                                                                         aaaCGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgcgc                                              >  2:1612786/1‑140 (MQ=255)                                                                                         aaaCGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgcgc                                              >  2:1084387/1‑140 (MQ=255)                                                                                         aaaCGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgcgc                                              >  1:566771/1‑140 (MQ=255)                                                                                            cGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgc                                                >  1:1415899/1‑135 (MQ=255)                                                                                            cGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGAGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgc                                                <  2:1415899/135‑1 (MQ=255)                                                                                              ccATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTcc                                         <  2:363025/140‑1 (MQ=255)                                                                                              ccATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTcc                                         <  1:1514031/140‑1 (MQ=255)                                                                                              ccATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTcc                                         <  2:166679/140‑1 (MQ=255)                                                                                              ccATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTcc                                         <  2:602289/140‑1 (MQ=255)                                                                                                 tAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAg                                      <  1:1612786/140‑1 (MQ=255)                                                                                                   aCATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGt                                    >  1:948134/1‑140 (MQ=255)                                                                                                     aTCGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCt                                  <  1:1074275/140‑1 (MQ=255)                                                                                                       cGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGa                                <  2:458746/140‑1 (MQ=255)                                                                                                       cGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGa                                <  1:863611/140‑1 (MQ=255)                                                                                                       cGTGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGa                                <  1:213774/140‑1 (MQ=255)                                                                                                        gTGGCGATTCCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGaa                               >  1:774381/1‑140 (MQ=255)                                                                                                         tGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgc                                                <  1:562856/122‑1 (MQ=255)                                                                                                         tGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCcgc                                                >  2:562856/1‑122 (MQ=255)                                                                                                         tGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAAt                              >  2:1192912/1‑140 (MQ=255)                                                                                                         tGGCGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAAt                              >  2:489454/1‑140 (MQ=255)                                                                                                            cGATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGc                           <  1:736432/140‑1 (MQ=255)                                                                                                             gATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCa                          <  1:532046/140‑1 (MQ=255)                                                                                                             gATTGCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCa                          <  2:1058791/140‑1 (MQ=255)                                                                                                                 gCCGATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGa                      <  2:588661/140‑1 (MQ=255)                                                                                                                    gATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCt                   <  2:1506187/140‑1 (MQ=255)                                                                                                                    gATATCGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCt                   <  2:829708/140‑1 (MQ=255)                                                                                                                         cGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCaa              <  2:670376/140‑1 (MQ=255)                                                                                                                         cGATACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCaa              <  1:337794/140‑1 (MQ=255)                                                                                                                            tACCCGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAaag           >  1:884483/1‑140 (MQ=255)                                                                                                                               ccGTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAaagaag        >  1:1142405/1‑140 (MQ=255)                                                                                                                                 gTAAGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTg      >  1:494619/1‑140 (MQ=255)                                                                                                                                   aaGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGac    <  2:846850/140‑1 (MQ=255)                                                                                                                                   aaGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGac    <  2:884483/140‑1 (MQ=255)                                                                                                                                   aaGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGac    <  2:948134/140‑1 (MQ=255)                                                                                                                                    aGCTGGCGCGTTTAGTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGacc   <  2:79730/140‑1 (MQ=255)                                                                                                                                    aGCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGacc   <  1:932701/140‑1 (MQ=255)                                                                                                                                     gCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGacca  <  1:387938/140‑1 (MQ=255)                                                                                                                                     gCTGGCGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGacca  <  1:1084387/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCTTACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGACGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCA  >  NC_000913/29869‑30140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑