Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,033,930 C→T E415E (GAG→GAA)  lysS ← lysine‑‑tRNA ligase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,033,9300CT100.0% 375.7 / NA 105E415E (GAG→GAA) lysSlysine‑‑tRNA ligase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/54);  total (51/54)

Only 100 of 106 total aligned reads displayed.

ATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATA  >  NC_000913/3033795‑3034065
                                                                                                                                       |                                                                                                                                       
aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCaa                                                                                                                                     >  2:462096/1‑140 (MQ=255)
aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCaa                                                                                                                                     >  1:1438967/1‑140 (MQ=255)
aTCTTCATCGTAGAACATCGCTTCGGCGGCCCCTGTGTCCTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGCCCTTCCGCTCGCTAAAGCCGTTACCGACTTCACGACCACCAATGAAGAATTcct                                                                                                                                     >  2:474643/1‑138 (MQ=255)
     cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCgg                                                                                                                                >  1:952234/1‑140 (MQ=255)
       tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTc                                                                                                                              >  2:772539/1‑140 (MQ=255)
        cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCt                                                                                                                             >  1:1104971/1‑140 (MQ=255)
          tAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCtgt                                                                                                                           >  1:1060360/1‑140 (MQ=255)
           aGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCtgtg                                                                                                                          >  2:688881/1‑140 (MQ=255)
             aaCATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGAt                                                                                                                        >  1:796966/1‑140 (MQ=255)
               cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGAttt                                                                                                                      <  1:1447675/140‑1 (MQ=255)
                aTCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTc                                                                                                                     <  1:53422/140‑1 (MQ=255)
                aTCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTc                                                                                                                     <  1:175269/140‑1 (MQ=255)
                aTCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTc                                                                                                                     <  2:378369/140‑1 (MQ=255)
                 tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGGCCACCAATGAAGAATTCAAAGCGGTCTGTGATTTcc                                                                                                                    <  1:1376564/140‑1 (MQ=255)
                  cGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCg                                                                                                                   >  1:127756/1‑140 (MQ=255)
                  cGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCg                                                                                                                   >  2:246744/1‑140 (MQ=255)
                  cGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCg                                                                                                                   >  2:1164318/1‑140 (MQ=255)
                  cGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCg                                                                                                                   >  2:435661/1‑140 (MQ=255)
                  cGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCg                                                                                                                   >  1:204435/1‑140 (MQ=255)
                  cGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCg                                                                                                                   >  2:871754/1‑140 (MQ=255)
                    cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCggg                                                                                                                 >  2:1025423/1‑140 (MQ=255)
                    cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCggg                                                                                                                 <  2:877474/140‑1 (MQ=255)
                       cgtcgtCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTa                                                                                                              >  1:1273974/1‑140 (MQ=255)
                        gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTaa                                                                                                             <  2:1273974/140‑1 (MQ=255)
                        gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTaa                                                                                                             <  2:899001/140‑1 (MQ=255)
                         tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAAc                                                                                                            >  2:800119/1‑140 (MQ=255)
                          cgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAAcg                                                                                                           <  1:1025423/140‑1 (MQ=255)
                            tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtc                                                                                                         >  2:1063927/1‑140 (MQ=255)
                            tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtc                                                                                                         >  2:1520773/1‑140 (MQ=255)
                                   cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacga                                                                                                  <  2:127756/140‑1 (MQ=255)
                                   cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacga                                                                                                  >  1:57167/1‑140 (MQ=255)
                                               cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGga                                                                                      <  2:1046051/140‑1 (MQ=255)
                                                 aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga                                                                                    <  2:200695/140‑1 (MQ=255)
                                                           ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGcc                                                                          >  2:440986/1‑140 (MQ=255)
                                                           ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGcc                                                                          >  1:1474646/1‑140 (MQ=255)
                                                           ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGcc                                                                          >  1:1088576/1‑140 (MQ=255)
                                                           ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGcc                                                                          >  2:81280/1‑140 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg                                                                        <  1:1164318/140‑1 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg                                                                        <  1:1091641/140‑1 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg                                                                        >  2:803018/1‑140 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg                                                                        <  1:329980/140‑1 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg                                                                        <  1:1071238/140‑1 (MQ=255)
                                                             aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg                                                                        <  1:871754/140‑1 (MQ=255)
                                                                cGTTGTGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                                                     <  2:1438967/140‑1 (MQ=255)
                                                                cGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                                                     >  1:1082901/1‑140 (MQ=255)
                                                                cGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                                                     >  1:467668/1‑140 (MQ=255)
                                                                cGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                                                     >  2:280561/1‑140 (MQ=255)
                                                                cGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                                                     >  2:1544576/1‑140 (MQ=255)
                                                                 gTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGatat                                                                    >  2:826008/1‑140 (MQ=255)
                                                                  ttGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt                                                                   >  2:1599614/1‑140 (MQ=255)
                                                                   tGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTc                                                                  <  1:280561/140‑1 (MQ=255)
                                                                   tGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTc                                                                  >  1:205449/1‑140 (MQ=255)
                                                                   tGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTc                                                                  <  1:1544576/140‑1 (MQ=255)
                                                                     cgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                                                     >  1:337941/1‑135 (MQ=255)
                                                                     cgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                                                     <  2:337941/135‑1 (MQ=255)
                                                                     cgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAg                                                                >  1:257167/1‑140 (MQ=255)
                                                                          gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg                                                           >  2:781857/1‑140 (MQ=255)
                                                                          gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg                                                           >  2:347700/1‑140 (MQ=255)
                                                                               tCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt                                                      <  2:1081508/140‑1 (MQ=255)
                                                                                 cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAgg                                                       <  1:1076093/137‑1 (MQ=255)
                                                                                 cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAgg                                                       >  2:1076093/1‑137 (MQ=255)
                                                                                  gcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTc                                                                  <  2:536991/125‑1 (MQ=255)
                                                                                  gcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTc                                                                  >  1:536991/1‑125 (MQ=255)
                                                                                  gcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCgg                                                   >  1:470021/1‑140 (MQ=255)
                                                                                  gcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCg                                                    <  2:757161/139‑1 (MQ=255)
                                                                                  gcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCg                                                    >  1:757161/1‑139 (MQ=255)
                                                                                   cgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGc                                                  <  1:170223/140‑1 (MQ=255)
                                                                                   cgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGc                                                  <  2:57167/140‑1 (MQ=255)
                                                                                    gTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt                                                 <  1:246744/140‑1 (MQ=255)
                                                                                        ttCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt                                             <  1:435661/140‑1 (MQ=255)
                                                                                        ttCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt                                             <  1:81280/140‑1 (MQ=255)
                                                                                        ttCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt                                             <  2:30305/140‑1 (MQ=255)
                                                                                            gCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa                                         <  2:1474646/140‑1 (MQ=255)
                                                                                             cTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt                                        <  2:1082901/140‑1 (MQ=255)
                                                                                             cTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt                                        <  2:1088576/140‑1 (MQ=255)
                                                                                             cTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt                                        <  2:1104971/140‑1 (MQ=255)
                                                                                                  tAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCt                                   >  2:1263807/1‑140 (MQ=255)
                                                                                                  tAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCt                                   >  2:729467/1‑140 (MQ=255)
                                                                                                  tAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCt                                   <  1:1063927/140‑1 (MQ=255)
                                                                                                  tAAAGCCGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCt                                   <  1:781857/140‑1 (MQ=255)
                                                                                                       ccGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGc                              <  2:257167/140‑1 (MQ=255)
                                                                                                       ccGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGc                              <  1:688881/140‑1 (MQ=255)
                                                                                                       ccGTTACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGc                              >  2:1340876/1‑140 (MQ=255)
                                                                                                          ttACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCa                            >  2:1458297/1‑139 (MQ=255)
                                                                                                          ttACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCa                            <  1:1458297/139‑1 (MQ=255)
                                                                                                          ttACCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc                           >  1:701309/1‑140 (MQ=255)
                                                                                                            aCCGATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActt                         >  1:237926/1‑140 (MQ=255)
                                                                                                               gATTTCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtctt                      <  2:796966/140‑1 (MQ=255)
                                                                                                                 tttCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg                    <  2:690524/140‑1 (MQ=255)
                                                                                                                 tttCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg                    <  2:701309/140‑1 (MQ=255)
                                                                                                                  ttCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa                   >  1:228876/1‑140 (MQ=255)
                                                                                                                  ttCACGACCACCAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa                   >  2:763058/1‑140 (MQ=255)
                                                                                                                        accaccAATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct             <  2:237926/140‑1 (MQ=255)
                                                                                                                          caccaATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg           >  2:502805/1‑140 (MQ=255)
                                                                                                                           accaATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg          >  1:572817/1‑140 (MQ=255)
                                                                                                                            ccaATGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt         <  1:1599614/140‑1 (MQ=255)
                                                                                                                              aaTGAAGAATTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTaa       <  1:800119/140‑1 (MQ=255)
                                                                                                                                   agaaTTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa  <  1:440986/140‑1 (MQ=255)
                                                                                                                                   agaaTTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa  <  1:772539/140‑1 (MQ=255)
                                                                                                                                   agaaTTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa  <  2:120134/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
ATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATA  >  NC_000913/3033795‑3034065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑