Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,355,610	G→A	D296N (GAT→AAT)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,355,610	0	G	A	100.0%	318.4 / NA	88	D296N (GAT→AAT)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/51);  total (37/51)

CGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCCGGCGGGCATC  >  NC_000913/3355476‑3355741                                                                                                                                       |                                                                                                                                    cgcgtgcgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATatc                                                                                                                                >  1:229910/1‑140 (MQ=255) cgcgtgcgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATatc                                                                                                                                >  1:996321/1‑140 (MQ=255)   cgtgcgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATatcat                                                                                                                              >  2:938149/1‑140 (MQ=255)    gtgcgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATatcatc                                                                                                                             <  1:439006/140‑1 (MQ=255)    gtgcgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATatcatc                                                                                                                             <  1:756907/140‑1 (MQ=255)       cgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCcgc                                                                                                                          >  1:444621/1‑140 (MQ=255)         cgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCgcgc                                                                                                                        >  2:1045045/1‑140 (MQ=255)          gtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACCCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGcc                                                                                                                       <  1:771070/140‑1 (MQ=255)             cTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATg                                                                                                                    <  1:1047657/140‑1 (MQ=255)             cTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATg                                                                                                                    <  1:631155/140‑1 (MQ=255)               tataAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCg                                                                                                                  <  1:1598795/140‑1 (MQ=255)               tataAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCg                                                                                                                  <  1:266206/140‑1 (MQ=255)               tataAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCg                                                                                                                  <  1:812082/140‑1 (MQ=255)               tataAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCg                                                                                                                  <  1:938149/140‑1 (MQ=255)               tataAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCg                                                                                                                  <  2:444621/140‑1 (MQ=255)                ataAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGt                                                                                                                 >  2:1132886/1‑140 (MQ=255)                ataAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGt                                                                                                                 >  1:1075758/1‑140 (MQ=255)                   aaTTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTa                                                                                                              <  2:958431/140‑1 (MQ=255)                   aaTTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACCGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTa                                                                                                              <  2:1446423/140‑1 (MQ=255)                    aTTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTAt                                                                                                             <  1:1132886/140‑1 (MQ=255)                        cAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGc                                                                                                                   <  2:890302/130‑1 (MQ=255)                        cAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGc                                                                                                                   >  1:890302/1‑130 (MQ=255)                        cAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCg                                                                                                                  >  2:612565/1‑131 (MQ=255)                        cAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCg                                                                                                                  <  1:612565/131‑1 (MQ=255)                        cAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGt                                                                                                         >  2:160591/1‑140 (MQ=255)                        cAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGt                                                                                                         >  2:520320/1‑140 (MQ=255)                        cAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGt                                                                                                         >  2:723147/1‑140 (MQ=255)                               cGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTacc                                                                                                      >  2:1221807/1‑136 (MQ=255)                               cGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTacc                                                                                                      <  1:1221807/136‑1 (MQ=255)                               cGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCAccc                                                                                                  >  2:89946/1‑140 (MQ=255)                                gCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCg                                                                                                 <  1:1317305/140‑1 (MQ=255)                                     tCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTgg                                                                                            <  1:520320/140‑1 (MQ=255)                                        cTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTTCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAg                                                                                         <  2:1258273/140‑1 (MQ=255)                                         tGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGa                                                                                        <  1:6662/140‑1 (MQ=255)                                          gACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGaa                                                                                       <  2:621501/140‑1 (MQ=255)                                          gACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGaa                                                                                       <  2:560321/140‑1 (MQ=255)                                           aCCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGaac                                                                                      <  2:541860/140‑1 (MQ=255)                                           aCCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGaac                                                                                      <  2:1233319/140‑1 (MQ=255)                                           aCCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGaac                                                                                      <  2:1075758/140‑1 (MQ=255)                                           aCCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGaac                                                                                      <  2:17122/140‑1 (MQ=255)                                              tGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGaacaac                                                                                   >  2:1174795/1‑140 (MQ=255)                                               gCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAAcc                                                                                  >  1:1120341/1‑140 (MQ=255)                                                cACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAAccc                                                                                 >  1:319056/1‑140 (MQ=255)                                                cACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAAccc                                                                                 <  2:814323/140‑1 (MQ=255)                                                cACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAAccc                                                                                 >  2:784080/1‑140 (MQ=255)                                                     cgccgcACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGata                                                                            >  1:33780/1‑140 (MQ=255)                                                      gccgcACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGatat                                                                           <  1:586988/140‑1 (MQ=255)                                                      gccgcACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGatat                                                                           <  1:1509479/140‑1 (MQ=255)                                                        cgcACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATgg                                                                         <  2:229910/140‑1 (MQ=255)                                                           aCCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGAcc                                                                      <  2:33780/140‑1 (MQ=255)                                                                     aaCGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCg                                                            <  2:923496/140‑1 (MQ=255)                                                                               gCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCttt                                                  <  2:996321/140‑1 (MQ=255)                                                                               gCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCttt                                                  <  1:1159088/140‑1 (MQ=255)                                                                                    gACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACtt                                             >  1:434193/1‑140 (MQ=255)                                                                                     aCTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACttt                                            >  2:556688/1‑140 (MQ=255)                                                                                     aCTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACttt                                            >  1:1263328/1‑140 (MQ=255)                                                                                     aCTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACttt                                            <  2:434193/140‑1 (MQ=255)                                                                                          aGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTc                                       <  1:784080/140‑1 (MQ=255)                                                                                          aGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTc                                       <  2:537222/140‑1 (MQ=255)                                                                                           gTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTcc                                      <  1:1430005/140‑1 (MQ=255)                                                                                             tCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTcc                                      >  1:1483133/1‑138 (MQ=255)                                                                                             tCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTcc                                      <  2:1483133/138‑1 (MQ=255)                                                                                             tCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCAt                                    <  2:1263328/140‑1 (MQ=255)                                                                                             tCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCAt                                    >  1:455424/1‑140 (MQ=255)                                                                                                   gATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCatat                              <  2:1199777/140‑1 (MQ=255)                                                                                                    aTAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATg                             <  1:213672/140‑1 (MQ=255)                                                                                                    aTAACATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATg                             <  1:220459/140‑1 (MQ=255)                                                                                                       aCATGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAg                          <  1:556688/140‑1 (MQ=255)                                                                                                         aTGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGcc                        <  2:455424/140‑1 (MQ=255)                                                                                                          tGCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCg                       <  1:1311653/140‑1 (MQ=255)                                                                                                           gCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGt                      >  1:1170874/1‑140 (MQ=255)                                                                                                           gCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGt                      >  1:1367575/1‑140 (MQ=255)                                                                                                           gCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGt                      >  2:1573747/1‑140 (MQ=255)                                                                                                           gCTGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACCGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGt                      >  2:1147890/1‑140 (MQ=255)                                                                                                             tGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTgg                    >  2:940268/1‑140 (MQ=255)                                                                                                             tGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTgg                    >  2:1441540/1‑140 (MQ=255)                                                                                                             tGGAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCGTCTTTGACTTTAACTCCATGCATATGGAGCCGTgg                    >  2:922751/1‑140 (MQ=255)                                                                                                              ggAACTGCTGCTGGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTggg                   >  1:833255/1‑140 (MQ=255)                                                                                                                   tgctgctgGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGc              <  1:968297/140‑1 (MQ=255)                                                                                                                     ctgctgGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGccc            >  2:60718/1‑140 (MQ=255)                                                                                                                     ctgctgGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGccc            >  1:1170254/1‑140 (MQ=255)                                                                                                                     ctgctgGCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGccc            >  1:1436004/1‑140 (MQ=255)                                                                                                                          ggCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCTTGCATATGGAGCCGTGGGATGGCCcggcgg       >  2:340856/1‑140 (MQ=255)                                                                                                                          ggCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCcggcgg       <  1:89946/140‑1 (MQ=255)                                                                                                                          ggCAGGCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCcggcgg       <  1:940268/140‑1 (MQ=255)                                                                                                                               gCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGccc            >  1:1426391/1‑130 (MQ=255)                                                                                                                               gCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGccc            <  2:1426391/130‑1 (MQ=255)                                                                                                                               gCGGGATGAATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCCGGCGGGCATc  <  2:1170254/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    CGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGTCTATTAGTACCACCCGCCTGGCAGAACAACCCGGATATGGACCCGGAACTGCGTGCCTTCTTTGACTTTAACTCCATGCATATGGAGCCGTGGGATGGCCCGGCGGGCATC  >  NC_000913/3355476‑3355741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑