Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,524,569	T→C	S567P (TCC→CCC)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,524,569	0	T	C	100.0%	209.8 / NA	59	S567P (TCC→CCC)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/28);  total (31/28)

TGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGCC  >  NC_000913/3524432‑3524703                                                                                                                                          |                                                                                                                                       tGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGccc                                                                                                                                      >  1:455653/1‑140 (MQ=255)  gggTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCt                                                                                                                                     >  2:1313820/1‑140 (MQ=255)  gggTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCt                                                                                                                                     <  2:84246/140‑1 (MQ=255)  gggTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCt                                                                                                                                     >  1:84246/1‑140 (MQ=255)  gggTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCt                                                                                                                                     >  2:692813/1‑140 (MQ=255)   ggTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCtt                                                                                                                                    <  1:14315/140‑1 (MQ=255)   ggTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCtt                                                                                                                                    <  2:6514/140‑1 (MQ=255)    gTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTc                                                                                                                                   <  1:1502022/140‑1 (MQ=255)      cAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCAc                                                                                                                                 >  2:1257765/1‑140 (MQ=255)      cAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCAc                                                                                                                                 >  2:1227036/1‑140 (MQ=255)      cAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCAc                                                                                                                                 <  1:26834/140‑1 (MQ=255)         tCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCAcccc                                                                                                                              >  1:352788/1‑140 (MQ=255)         tCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCCTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACACTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCAcccc                                                                                                                              <  2:1117132/140‑1 (MQ=255)             aaaaCGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATg                                                                                                                          <  1:1091958/140‑1 (MQ=255)                          ggTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGcgcgcg                                                                                                             <  1:532745/140‑1 (MQ=255)                            tACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGc                                                                                                           >  1:396176/1‑140 (MQ=255)                              cgcgCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTa                                                                                                         >  2:139014/1‑140 (MQ=255)                                 gcAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTAcgc                                                                                                      <  2:352788/140‑1 (MQ=255)                                     tGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTc                                                                                                  >  1:336642/1‑140 (MQ=255)                                               gggCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAAcg                                                                                        >  1:205627/1‑140 (MQ=255)                                               gggCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAAcg                                                                                        >  2:214998/1‑140 (MQ=255)                                                ggCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAAcgg                                                                                       <  2:455653/140‑1 (MQ=255)                                                 gCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAAcggc                                                                                      >  1:1623310/1‑140 (MQ=255)                                                    tCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCTTGGCGAACggcggc                                                                                   >  2:1409665/1‑140 (MQ=255)                                                    tCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACggcggc                                                                                   <  2:220329/140‑1 (MQ=255)                                                      gACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCtt                                                                                 >  1:1176690/1‑140 (MQ=255)                                                           cGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCtgg                                                                            >  1:771302/1‑140 (MQ=255)                                                           cGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCtgg                                                                            <  2:338031/140‑1 (MQ=255)                                                               gCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGa                                                                        <  1:1227036/140‑1 (MQ=255)                                                                      atCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTgg                                                                 >  2:1332751/1‑140 (MQ=255)                                                                       tCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGt                                                                >  1:1273507/1‑140 (MQ=255)                                                                         tGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGttt                                                              <  2:610614/140‑1 (MQ=255)                                                                          gCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTa                                                             >  2:772789/1‑140 (MQ=255)                                                                               gCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGc                                                        <  1:1505287/140‑1 (MQ=255)                                                                                 ttCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTACTGGTGGACCCGTGGTTTATCAGCaa                                                      >  2:539503/1‑140 (MQ=255)                                                                                   cGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCaaaa                                                    >  2:502646/1‑140 (MQ=255)                                                                                    ggCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAAt                                                   <  1:1300028/140‑1 (MQ=255)                                                                                     gCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAAtt                                                  >  1:1104605/1‑140 (MQ=255)                                                                                        tCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGaa                                               <  2:1498835/140‑1 (MQ=255)                                                                                         cccGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGaaa                                              <  1:141938/140‑1 (MQ=255)                                                                                          ccGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGaaaa                                             >  2:565116/1‑140 (MQ=255)                                                                                             gCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGa                                          <  2:205627/140‑1 (MQ=255)                                                                                             gCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGa                                          <  2:1176690/140‑1 (MQ=255)                                                                                              cacaAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGAt                                         >  1:474588/1‑140 (MQ=255)                                                                                                  aaaCATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAgg                                     <  2:474588/140‑1 (MQ=255)                                                                                                   aaCATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAggg                                    <  2:1104605/140‑1 (MQ=255)                                                                                                         gTCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGt                              >  1:547831/1‑140 (MQ=255)                                                                                                          tCCACACCGAATCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGGGGTTTATCGGCAAAATTGAAAACGACAAGggcgggggg                             >  2:32927/1‑136 (MQ=255)                                                                                                                   aaTCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGaa                    >  1:882638/1‑140 (MQ=255)                                                                                                                   aaTCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGaa                    <  1:867451/140‑1 (MQ=255)                                                                                                                    aTCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTATGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAg                   >  1:950205/1‑140 (MQ=255)                                                                                                                    aTCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAg                   >  1:608180/1‑140 (MQ=255)                                                                                                                     tCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTATGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGc                  <  2:950205/140‑1 (MQ=255)                                                                                                                     tCGCTGGCGCTGGGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGc                  <  2:608180/140‑1 (MQ=255)                                                                                                                      cgctggcgctggGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCg                 <  1:772789/140‑1 (MQ=255)                                                                                                                         tggcgctggGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGaaa              >  2:614526/1‑140 (MQ=255)                                                                                                                         tggcgctggGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGaaa              >  2:779128/1‑140 (MQ=255)                                                                                                                          ggcgctggGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAAc             <  2:882638/140‑1 (MQ=255)                                                                                                                               tggGTTCAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGaaa        <  1:625140/140‑1 (MQ=255)                                                                                                                                     cAGCGCCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGcc  >  1:1533087/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCACAAAACATTGTCCACACCGAATCGCTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTGGTGGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGCC  >  NC_000913/3524432‑3524703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑