Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,529,992	T→C	G73G (GGT→GGC)	hslO →	molecular chaperone Hsp33

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,529,992	0	T	C	100.0%	353.3 / NA	98	G73G (GGT→GGC)	hslO	molecular chaperone Hsp33
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/51);  total (47/51)

TGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACC  >  NC_000913/3529856‑3530127                                                                                                                                         |                                                                                                                                        tGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCg                                                                                                                                      >  1:1326653/1‑140 (MQ=255)  gCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGa                                                                                                                                     <  2:572141/140‑1 (MQ=255)   cAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGAt                                                                                                                                    <  1:1572440/140‑1 (MQ=255)        gATCCTTGAGAACCACGATTATCCGCCGCCCGCTAAAAACCTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATc                                                                                                                               <  2:1437240/140‑1 (MQ=255)         aTCCTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCt                                                                                                                              >  2:503373/1‑140 (MQ=255)           ccTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTATAGCTGCAGGGCGACGGCCCGATGAATCTgg                                                                                                                            >  2:206371/1‑140 (MQ=255)           ccTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTgg                                                                                                                            <  1:935165/140‑1 (MQ=255)           ccTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTgg                                                                                                                            <  1:503373/140‑1 (MQ=255)           ccTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTACTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTgg                                                                                                                            <  1:767537/140‑1 (MQ=255)             ttGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCg                                                                                                                          <  1:742650/140‑1 (MQ=255)              tGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCgg                                                                                                                         <  2:1595001/140‑1 (MQ=255)                agaACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATGACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGtt                                                                                                                       >  2:1516219/1‑140 (MQ=255)                  aaCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGttat                                                                                                                     >  2:840227/1‑140 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCTACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGAt                                                                                                                                    >  1:915940/1‑124 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCTACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGAt                                                                                                                                    <  2:915940/124‑1 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGAt                                                                                                                                    <  1:507978/124‑1 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGAt                                                                                                                                    >  2:507978/1‑124 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCgg                                                                                                                         >  1:779204/1‑135 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCgg                                                                                                                         <  2:779204/135‑1 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGttatt                                                                                                                    <  2:934491/140‑1 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGttatt                                                                                                                    >  1:227155/1‑140 (MQ=255)                   aCCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGttatt                                                                                                                    <  2:227155/140‑1 (MQ=255)                     cACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTATAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTaa                                                                                                                  <  1:206371/140‑1 (MQ=255)                     cACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTaa                                                                                                                  >  1:1010256/1‑140 (MQ=255)                       cGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACg                                                                                                                >  1:1445042/1‑140 (MQ=255)                        gATTATCCGCAGCCCGTTAAAACCGTGCTGGAAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACgg                                                                                                               <  2:1424271/140‑1 (MQ=255)                        gATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTTATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACgg                                                                                                               <  2:1596427/140‑1 (MQ=255)                        gATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACgg                                                                                                               <  1:331573/140‑1 (MQ=255)                        gATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACgg                                                                                                               <  2:925044/140‑1 (MQ=255)                           tATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTaa                                                                                                            <  2:716802/140‑1 (MQ=255)                             tCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACa                                                                                                          >  2:1519059/1‑140 (MQ=255)                               cGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAAt                                                                                                        >  2:1554524/1‑140 (MQ=255)                                gCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATa                                                                                                       <  1:1129859/140‑1 (MQ=255)                                  aGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAAc                                                                                                     >  1:1429555/1‑140 (MQ=255)                                    cccGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAAc                                                                                                     >  1:62425/1‑138 (MQ=255)                                    cccGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAAc                                                                                                     <  2:62425/138‑1 (MQ=255)                                      cGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACcagc                                                                                                 >  2:1093250/1‑140 (MQ=255)                                       gTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACcagca                                                                                                >  2:1262063/1‑140 (MQ=255)                                          aaaaaCGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGAt                                                                                             <  1:1519059/140‑1 (MQ=255)                                              aCGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATgcgc                                                                                         >  1:1588278/1‑140 (MQ=255)                                              aCGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATgcgc                                                                                         >  2:583563/1‑140 (MQ=255)                                                  gCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATa                                                                                                       <  1:1114549/122‑1 (MQ=255)                                                  gCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATa                                                                                                       >  2:1114549/1‑122 (MQ=255)                                                         gAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGgcgcg                                                                              >  2:92322/1‑140 (MQ=255)                                                          aaCTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGATGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGGTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGgcgcgc                                                                             >  1:1214035/1‑140 (MQ=255)                                                                tgGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAg                                                                       >  2:901936/1‑140 (MQ=255)                                                                tgGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAg                                                                       >  1:654871/1‑140 (MQ=255)                                                                tgGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAg                                                                       >  1:1396939/1‑140 (MQ=255)                                                                    tGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCg                                                                   <  2:1331238/140‑1 (MQ=255)                                                                         ccAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAAtt                                                              <  2:1326653/140‑1 (MQ=255)                                                                         ccAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAAtt                                                              <  2:1387649/140‑1 (MQ=255)                                                                         ccAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAAtt                                                              <  1:840227/140‑1 (MQ=255)                                                                            gCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCa                                                           >  1:983726/1‑140 (MQ=255)                                                                            gCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGT‑ACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAg                                                          >  2:941258/1‑140 (MQ=255)                                                                                 ttAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGaaaa                                                      >  2:373685/1‑140 (MQ=255)                                                                                   aaCCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATg                                                    <  2:1429555/140‑1 (MQ=255)                                                                                        cTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGATGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGAc                                               <  2:1237547/140‑1 (MQ=255)                                                                                        cTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGAc                                               <  2:1445042/140‑1 (MQ=255)                                                                                           cGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTg                                            >  2:663579/1‑140 (MQ=255)                                                                                           cGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTg                                            >  2:541667/1‑140 (MQ=255)                                                                                           cGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTg                                            >  2:115611/1‑140 (MQ=255)                                                                                                  gTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGc                                     <  2:654871/140‑1 (MQ=255)                                                                                                    ttGATGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTg                                   <  2:1396939/140‑1 (MQ=255)                                                                                                       aTGGTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTc                                >  1:364812/1‑140 (MQ=255)                                                                                                         ggTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCgg                              <  2:1588278/140‑1 (MQ=255)                                                                                                         ggTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCgg                              <  1:663579/140‑1 (MQ=255)                                                                                                          gTGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGc                             >  1:263975/1‑140 (MQ=255)                                                                                                           tGATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGTCGACCTGAAAACGCTGGTCGGCa                            <  2:1361797/140‑1 (MQ=255)                                                                                                            gATATCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCaa                           <  2:1295023/140‑1 (MQ=255)                                                                                                               atCACCGTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATgg                        <  2:245896/140‑1 (MQ=255)                                                                                                                     gTACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACgt                  >  2:1275980/1‑140 (MQ=255)                                                                                                                      tACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCaa                           >  2:1404955/1‑130 (MQ=255)                                                                                                                      tACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCaa                           <  1:1404955/130‑1 (MQ=255)                                                                                                                      tACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACgtg                 <  1:541667/140‑1 (MQ=255)                                                                                                                      tACAGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACgtg                 <  1:1554524/140‑1 (MQ=255)                                                                                                                         aGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACgtggtg              >  1:633313/1‑140 (MQ=255)                                                                                                                         aGCTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACgtggtg              <  1:115611/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  2:587616/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  2:983726/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            >  2:1262388/1‑140 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  1:583563/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  1:1093250/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            >  1:1203189/1‑140 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  1:1262063/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  1:1335212/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  1:1516219/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  2:364812/140‑1 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            >  2:1359172/1‑140 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            >  2:1553966/1‑140 (MQ=255)                                                                                                                           cTGCAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            >  2:172837/1‑140 (MQ=255)                                                                                                                              cAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACg                   >  1:723427/1‑130 (MQ=255)                                                                                                                              cAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACg                   <  2:723427/130‑1 (MQ=255)                                                                                                                              cAGGGCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCAc         >  1:481955/1‑140 (MQ=255)                                                                                                                                gggCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            <  2:1132650/135‑1 (MQ=255)                                                                                                                                gggCGACGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGAt            >  1:1132650/1‑135 (MQ=255)                                                                                                                                     aCGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTAcc  >  1:249688/1‑140 (MQ=255)                                                                                                                                     aCGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTAcc  >  2:141260/1‑140 (MQ=255)                                                                                                                                     aCGGCCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTAcc  >  2:323905/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGCAACAGATCCTTGAGAACCACGATTATCCGCAGCCCGTTAAAAACGTGCTGGCAGAACTGCTGGTTGCGACCAGCCTGTTAACCGCTACGCTGAAGTTTGATGGTGATATCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACC  >  NC_000913/3529856‑3530127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑