BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,630,810	A→G	intergenic (‑208/+158)	yhiI ← / ← yhiJ	putative membrane fusion protein YhiI/DUF4049 domain‑containing protein YhiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,630,810	0	A	G	100.0%	419.8 / NA	115	intergenic (‑208/+158)	yhiI/yhiJ	putative membrane fusion protein YhiI/DUF4049 domain‑containing protein YhiJ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (55/60); total (55/60)

Only 100 of 116 total aligned reads displayed.

TCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCAGATAGT  >  NC_000913/3630674‑3630940

 cttcCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGTCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCa                                                                                                                                <  2:1423444/140‑1 (MQ=255)

 cttcCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCa                                                                                                                                <  1:1241584/140‑1 (MQ=255)

     cATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAg                                                                                                                            >  1:718876/1‑140 (MQ=255)

       tCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAgtg                                                                                                                          >  2:244077/1‑140 (MQ=255)

         cGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGtgtg                                                                                                                        <  2:1014593/140‑1 (MQ=255)

         cGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGtgtg                                                                                                                        <  1:244077/140‑1 (MQ=255)

          gggATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGa                                                                                                                       >  1:87558/1‑140 (MQ=255)

            gATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATc                                                                                                                     >  2:806554/1‑140 (MQ=255)

            gATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATc                                                                                                                     >  1:474989/1‑140 (MQ=255)

              tAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCta                                                                                                                   >  2:910795/1‑140 (MQ=255)

                 gaagGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATaa                                                                                                                <  1:565711/140‑1 (MQ=255)

                   agGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATaaaa                                                                                                              <  2:348004/140‑1 (MQ=255)

                      aaaCGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTg                                                                                                           <  1:692127/140‑1 (MQ=255)

                      aaaCGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTg                                                                                                           >  1:1471874/1‑140 (MQ=255)

                      aaaCGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTg                                                                                                           >  1:1492836/1‑140 (MQ=255)

                      aaaCGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTg                                                                                                           >  2:692127/1‑140 (MQ=255)

                       aaCGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCGAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATaaa                                                                                                               <  1:1269238/135‑1 (MQ=255)

                       aaCGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCGAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATaaa                                                                                                               >  2:1269238/1‑135 (MQ=255)

                         cGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCt                                                                                                        >  2:1397591/1‑140 (MQ=255)

                               aTACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACaaa                                                                                                  <  2:31991/140‑1 (MQ=255)

                                    aCCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGt                                                                                             >  1:536514/1‑140 (MQ=255)

                                        gcgcgcAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatcc                                                                                         >  1:715422/1‑140 (MQ=255)

                                         cgcgcAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatcca                                                                                        <  1:392604/140‑1 (MQ=255)

                                         cgcgcAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatcca                                                                                        <  1:517947/140‑1 (MQ=255)

                                           cgcAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatccatc                                                                                      <  2:497049/140‑1 (MQ=255)

                                           cgcAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatccatc                                                                                      >  1:70029/1‑140 (MQ=255)

                                             cAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatc                                                                                          >  2:71244/1‑134 (MQ=255)

                                             cAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCt                                                                                    <  2:1471874/140‑1 (MQ=255)

                                               gagCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGt                                                                                  >  2:537655/1‑140 (MQ=255)

                                                   aTTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGa                                                                              >  2:69135/1‑140 (MQ=255)

                                                      gAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAg                                                                           <  2:87558/140‑1 (MQ=255)

                                                      gAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAg                                                                           <  2:474989/140‑1 (MQ=255)

                                                      cactgcCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAg                                                                           <  1:951133/136‑1 (MQ=255)

                                                       aGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatcca                                                                                        <  1:1214161/126‑1 (MQ=255)

                                                       aGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatcca                                                                                        >  2:1214161/1‑126 (MQ=255)

                                                       aGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGc                                                                          <  1:1244784/140‑1 (MQ=255)

                                                       aGCGCCAACGGGGAAGGCTTAGAGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGc                                                                          >  1:892106/1‑140 (MQ=255)

                                                          gcCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatccatc                                                                                      >  1:88273/1‑125 (MQ=255)

                                                          gcCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatccatc                                                                                      >  2:1362267/1‑125 (MQ=255)

                                                          gcCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatccatc                                                                                      <  1:1362267/125‑1 (MQ=255)

                                                          gcCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTatccatc                                                                                      <  2:88273/125‑1 (MQ=255)

                                                          gcCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGtt                                                                       >  1:1257296/1‑140 (MQ=255)

                                                           ccAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGttt                                                                      <  1:442741/140‑1 (MQ=255)

                                                            cAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTc                                                                     >  2:1270458/1‑140 (MQ=255)

                                                                     aGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGtt                                                            <  1:537655/140‑1 (MQ=255)

                                                                       gCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCa                                                          <  1:806554/140‑1 (MQ=255)

                                                                         ttAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGt                                                        <  1:753158/140‑1 (MQ=255)

                                                                         ttAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGt                                                        >  1:142500/1‑140 (MQ=255)

                                                                         ttAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGt                                                        <  1:1270458/140‑1 (MQ=255)

                                                                          tAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGtt                                                       >  2:1381839/1‑140 (MQ=255)

                                                                           aGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTg                                                      >  1:533868/1‑140 (MQ=255)

                                                                           aGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTg                                                      >  1:696368/1‑140 (MQ=255)

                                                                            gTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGc                                                     <  1:1612249/140‑1 (MQ=255)

                                                                                 aaCGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGaa                                                >  2:746854/1‑140 (MQ=255)

                                                                                 aaCGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGaa                                                <  1:746854/140‑1 (MQ=255)

                                                                                  aCGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCa                                                          <  1:1341446/129‑1 (MQ=255)

                                                                                  aCGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCa                                                          >  2:1341446/1‑129 (MQ=255)

                                                                                  aCGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAAc                                               >  1:788881/1‑140 (MQ=255)

                                                                                  aCGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGCGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAAc                                               >  2:1552888/1‑140 (MQ=255)

                                                                                          taATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCAtt                                       <  2:1257296/140‑1 (MQ=255)

                                                                                                tgtCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCtttt                                 <  1:69135/140‑1 (MQ=255)

                                                                                                tgtCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCtttt                                 >  1:502776/1‑140 (MQ=255)

                                                                                                tgtCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCtttt                                 >  2:1102361/1‑140 (MQ=255)

                                                                                                 gtCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCttttt                                >  2:1391594/1‑140 (MQ=255)

                                                                                                  tcatcatcaGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTa                               >  2:504446/1‑140 (MQ=255)

                                                                                                   catcatcaGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTAc                              <  2:536514/140‑1 (MQ=255)

                                                                                                    atcatcaGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACg                             >  1:1073045/1‑140 (MQ=255)

                                                                                                    atcatcaGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACg                             >  2:607937/1‑140 (MQ=255)

                                                                                                         caGCATTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTa                        >  1:817829/1‑140 (MQ=255)

                                                                                                             aTTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  2:788881/140‑1 (MQ=255)

                                                                                                             aTTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  2:70029/140‑1 (MQ=255)

                                                                                                             aTTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  2:586805/140‑1 (MQ=255)

                                                                                                             aTTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  1:559703/140‑1 (MQ=255)

                                                                                                             aTTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  1:607937/140‑1 (MQ=255)

                                                                                                             aTTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  2:111709/140‑1 (MQ=255)

                                                                                                             aTTCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  2:142500/140‑1 (MQ=255)

                                                                                                              ttCCTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAAc                   >  1:1368348/1‑140 (MQ=255)

                                                                                                                ccTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAg                 >  2:516910/1‑140 (MQ=255)

                                                                                                                ccTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAg                 >  1:877449/1‑140 (MQ=255)

                                                                                                                 cTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGt                <  2:877449/140‑1 (MQ=255)

                                                                                                                 cTGGCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGt                <  2:242833/140‑1 (MQ=255)

                                                                                                                    gCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGtctc             >  2:785645/1‑140 (MQ=255)

                                                                                                                    gCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGtctc             <  2:533868/140‑1 (MQ=255)

                                                                                                                    gCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGtctc             <  2:892106/140‑1 (MQ=255)

                                                                                                                    gCCGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGtctc             <  1:473277/140‑1 (MQ=255)

                                                                                                                     ccGTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCa            >  2:576415/1‑140 (MQ=255)

                                                                                                                       gTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAAc          <  2:1458796/140‑1 (MQ=255)

                                                                                                                       gTAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAAc          <  2:1307411/140‑1 (MQ=255)

                                                                                                                        tAAATGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAAcc         >  1:519895/1‑140 (MQ=255)

                                                                                                                         aaaTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    <  2:680477/128‑1 (MQ=255)

                                                                                                                         aaaTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCaa                    >  1:680477/1‑128 (MQ=255)

                                                                                                                         aaaTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACa                  >  1:904144/1‑130 (MQ=255)

                                                                                                                         aaaTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACa                  <  2:904144/130‑1 (MQ=255)

                                                                                                                         aaaTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCa        <  1:965794/140‑1 (MQ=255)

                                                                                                                         aaaTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCa        >  1:174940/1‑140 (MQ=255)

                                                                                                                         aaaTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCa        >  2:163739/1‑140 (MQ=255)

                                                                                                                           aTGAAACACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCAGa      <  1:1391594/140‑1 (MQ=255)

                                                                                                                               aaCACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCAGATAGt  <  1:474193/140‑1 (MQ=255)

                                                                                                                               aaCACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCAGATAGt  <  1:1381839/140‑1 (MQ=255)

                                                                                                                               aaCACGCATGCTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCAGATAGt  <  1:504446/140‑1 (MQ=255)

TCTTCCATCCGGGATAAGAAGGAAACGGCAAATACGACCAGCGCGCAGAGCATTGAGCGCCAACGGGGAAGGCTTAGTGAAAACGGGTAGTAATGTTGTCATCATCAGCATTCCTGGCCGTAAATGAAACACGCATACTCATAAGTGTGATCTATAAAACTGGCTGACAAAACGGTATCCATCCTGTTGGATAGCGTTTCACGCCTGTTCAGTTGCAGGAACAGAGCATTCCTTTTTACGTTTTATCAACAGTCTCAACCAGATAGT  >  NC_000913/3630674‑3630940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`