BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,889,260	A→G	E177E (GAA→GAG)	tnaA →	tryptophanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,889,260	0	A	G	99.2%	475.9 / ‑6.5	132	E177E (GAA→GAG)	tnaA	tryptophanase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (66/65); minor base T (1/0); total (67/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TCTAACTATTTCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAACGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATT  >  NC_000913/3889123‑3889395

tctAACTATTTCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGcg                                                                                                                                       <  1:146479/140‑1 (MQ=255)

tctAACTATTTCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGcg                                                                                                                                       <  1:43068/140‑1 (MQ=255)

         ttCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTga                                                                                                                              >  1:180004/1‑140 (MQ=255)

         ttCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTga                                                                                                                              >  2:18727/1‑140 (MQ=255)

          tCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTgaa                                                                                                                             >  2:581891/1‑140 (MQ=255)

          tCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTgaa                                                                                                                             >  2:268944/1‑140 (MQ=255)

              tGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGaagaag                                                                                                                         >  2:232174/1‑140 (MQ=255)

              tGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGaagaag                                                                                                                         >  1:306797/1‑140 (MQ=255)

              tGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGaagaag                                                                                                                         >  2:1164151/1‑140 (MQ=255)

                      cGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCg                                                                                                                 >  2:1393/1‑140 (MQ=255)

                      cGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCg                                                                                                                 >  1:526834/1‑140 (MQ=255)

                      cGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCg                                                                                                                 >  2:498907/1‑140 (MQ=255)

                        cAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGaa                                                                                                               <  2:526834/140‑1 (MQ=255)

                           ggCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTATGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATaa                                                                                                            >  2:1451731/1‑140 (MQ=255)

                           ggCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATaa                                                                                                            >  1:1127417/1‑140 (MQ=255)

                           ggCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATaa                                                                                                            >  1:1106260/1‑140 (MQ=255)

                           ggCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATaa                                                                                                            >  2:274812/1‑140 (MQ=255)

                            gCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTATGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAAc                                                                                                           <  1:1451731/140‑1 (MQ=255)

                            gCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAAc                                                                                                           <  2:357274/140‑1 (MQ=255)

                            gCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAAc                                                                                                           <  2:665534/140‑1 (MQ=255)

                            gCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAAc                                                                                                           <  1:274812/140‑1 (MQ=255)

                            gCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAAc                                                                                                           <  1:961867/140‑1 (MQ=255)

                              cATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGt                                                                                                         <  1:581891/140‑1 (MQ=255)

                              cATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGt                                                                                                         >  2:704525/1‑140 (MQ=255)

                               aTAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATGCGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTg                                                                                                        <  1:232174/140‑1 (MQ=255)

                               aTAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTg                                                                                                        <  1:704525/140‑1 (MQ=255)

                               aTAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTg                                                                                                        <  2:306797/140‑1 (MQ=255)

                                tAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGc                                                                                                       >  2:789378/1‑140 (MQ=255)

                                tAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGc                                                                                                       >  1:1039605/1‑140 (MQ=255)

                                 aGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGcc                                                                                                      >  1:552954/1‑140 (MQ=255)

                                     aGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGtat                                                                                                  >  1:354740/1‑140 (MQ=255)

                                       aTCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGta                                                                                                   >  1:312109/1‑137 (MQ=255)

                                       aTCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTatat                                                                                                >  2:316029/1‑140 (MQ=255)

                                       aTCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTatat                                                                                                >  2:624247/1‑140 (MQ=255)

                                       aTCAACGGCTGTACCGTGCGTAACGTCTATACCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTatat                                                                                                >  1:413909/1‑140 (MQ=255)

                                           aCGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGtt                                                                                            <  1:789378/140‑1 (MQ=255)

                                            cGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTg                                                                                           <  1:1103983/140‑1 (MQ=255)

                                                 gTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAAcc                                                                                      >  1:1542679/1‑140 (MQ=255)

                                                  tACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGtat                                                                                                  <  2:109427/127‑1 (MQ=255)

                                                  tACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCa                                                                                     >  2:764215/1‑140 (MQ=255)

                                                         cGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATc                                                                                   <  1:1387105/135‑1 (MQ=255)

                                                         cGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAg                                                                              >  2:1327457/1‑140 (MQ=255)

                                                          gTAACGTCTATATCAAAGAAGTCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGt                                                                             <  2:1508199/140‑1 (MQ=255)

                                                          gTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTATGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGt                                                                             <  1:318245/140‑1 (MQ=255)

                                                          gTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGt                                                                             <  1:764215/140‑1 (MQ=255)

                                                              cGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAAct                                                                         >  1:12228/1‑140 (MQ=255)

                                                                 cTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTg                                                                      >  2:748343/1‑140 (MQ=255)

                                                                   atatCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCAcc                                                                                <  1:1172897/128‑1 (MQ=255)

                                                                   atatCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCAcc                                                                                >  2:1172897/1‑128 (MQ=255)

                                                                        aaaGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCGTCACCAGTaa                                                                           >  1:503473/1‑128 (MQ=255)

                                                                         aagaagCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGt                                                                             <  2:384832/125‑1 (MQ=255)

                                                                         aagaagCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAggtggt                                                              >  1:699741/1‑140 (MQ=255)

                                                                         aagaagCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAggtggt                                                              <  1:804513/140‑1 (MQ=255)

                                                                         aagaagCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAggtggt                                                              >  1:257996/1‑140 (MQ=255)

                                                                         aagaagCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAggtggt                                                              <  2:293180/140‑1 (MQ=255)

                                                                           gaagCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCa                                                            <  2:776648/140‑1 (MQ=255)

                                                                            aagCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAg                                                           >  1:1090463/1‑140 (MQ=255)

                                                                              gCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGcc                                                         >  2:946220/1‑140 (MQ=255)

                                                                              gCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGcc                                                         >  1:1052064/1‑140 (MQ=255)

                                                                              gCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGcc                                                         >  1:85759/1‑140 (MQ=255)

                                                                               ccTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGc                                                          <  2:48330/138‑1 (MQ=255)

                                                                               ccTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCg                                                        <  2:354740/140‑1 (MQ=255)

                                                                               ccTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGAGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATAGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCg                                                        >  2:1333399/1‑140 (MQ=255)

                                                                                   cGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGttt                                                    >  1:1340706/1‑140 (MQ=255)

                                                                                     aTACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCa                                                  <  2:1052064/140‑1 (MQ=255)

                                                                                     aTACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCa                                                  <  1:385525/140‑1 (MQ=255)

                                                                                         gggCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTgg                                              >  2:909838/1‑140 (MQ=255)

                                                                                         gggCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTgg                                              >  2:840164/1‑140 (MQ=255)

                                                                                           gcgtgcgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCa                                            <  1:392345/140‑1 (MQ=255)

                                                                                              tgcgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAAc                                         >  2:1572495/1‑140 (MQ=255)

                                                                                              tgcgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAAc                                         >  2:1340744/1‑140 (MQ=255)

                                                                                              tgcgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAAc                                         >  1:433058/1‑140 (MQ=255)

                                                                                               gcgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACt                                        >  1:211582/1‑140 (MQ=255)

                                                                                               gcgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACt                                        >  2:1257907/1‑140 (MQ=255)

                                                                                                cgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACtt                                       >  1:138289/1‑140 (MQ=255)

                                                                                                cgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACtt                                       >  1:1352757/1‑140 (MQ=255)

                                                                                                cgtTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACtt                                       >  1:398993/1‑140 (MQ=255)

                                                                                                  ttACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTaa                                     <  2:544002/140‑1 (MQ=255)

                                                                                                  ttACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTaa                                     <  2:1359425/140‑1 (MQ=255)

                                                                                                     cGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAg                                  >  2:510529/1‑140 (MQ=255)

                                                                                                       aCTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCg                                <  1:498907/140‑1 (MQ=255)

                                                                                                       aCTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCg                                <  1:1308537/140‑1 (MQ=255)

                                                                                                       aCTTTAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCg                                <  1:1393/140‑1 (MQ=255)

                                                                                                          ttAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATg                             <  1:510529/140‑1 (MQ=255)

                                                                                                          ttAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATg                             >  2:1184879/1‑140 (MQ=255)

                                                                                                           tAAAGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGt                            >  2:1555763/1‑140 (MQ=255)

                                                                                                            aaaGGCAACTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTa                           <  2:1352757/140‑1 (MQ=255)

                                                                                                                    cTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAg                                  <  1:1076978/125‑1 (MQ=255)

                                                                                                                    cTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAg                                  >  2:1076978/1‑125 (MQ=255)

                                                                                                                    cTTTGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATcg                   >  1:1566250/1‑140 (MQ=255)

                                                                                                                      ttGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATcgcg                 <  2:1592157/140‑1 (MQ=255)

                                                                                                                       tGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCga                <  2:433058/140‑1 (MQ=255)

                                                                                                                       tGACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCga                <  1:790373/140‑1 (MQ=255)

                                                                                                                        gACCTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCgaa               <  1:909838/140‑1 (MQ=255)

                                                                                                                          ccTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCgaaga             >  2:911075/1‑140 (MQ=255)

                                                                                                                           cTTGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCgaagaa            <  1:143159/140‑1 (MQ=255)

                                                                                                                            ttGAGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGaaa           <  2:407287/140‑1 (MQ=255)

                                                                                                                               aGGGATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATAc        >  1:380278/1‑140 (MQ=255)

                                                                                                                                  gATTAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGat     <  2:380278/140‑1 (MQ=255)

                                                                                                                                     tAGAGCGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATAtt  <  1:1184879/140‑1 (MQ=255)

TCTAACTATTTCTTTGATACCACGCAGGGCCATAGCCAGATCAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGATACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTTGAGGGATTAGAACGCGGTATTGAAGAAGTTGGTCCGAATAACGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATT  >  NC_000913/3889123‑3889395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`