Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,039	T→C	K314K (AAA→AAG)	alsA ←	D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,039	0	T	C	100.0%	279.1 / NA	77	K314K (AAA→AAG)	alsA	D‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/40);  total (37/40)

GCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCC  >  NC_000913/4309905‑4310175                                                                                                                                       |                                                                                                                                         gCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATc                                                                                                                                     >  1:967115/1‑140 (MQ=255)    aTGGAAAAGTTGGGGAAAAAACCGTTATCACGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCAc                                                                                                                                  <  1:89520/140‑1 (MQ=255)     tGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACg                                                                                                                                 >  2:78993/1‑140 (MQ=255)     tGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACg                                                                                                                                 >  1:518856/1‑140 (MQ=255)     tGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACg                                                                                                                                 >  2:281277/1‑140 (MQ=255)     tGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACg                                                                                                                                 <  2:1132715/140‑1 (MQ=255)     tGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACg                                                                                                                                 <  1:23652/140‑1 (MQ=255)            gTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACa                                                                                                                          <  1:952012/140‑1 (MQ=255)              tGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGa                                                                                                                        <  1:78993/140‑1 (MQ=255)               ggggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGAc                                                                                                                       >  1:853402/1‑140 (MQ=255)                gggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACa                                                                                                                      >  1:219462/1‑140 (MQ=255)                 ggAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACaa                                                                                                                     <  1:209156/140‑1 (MQ=255)                  gAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAAt                                                                                                                    >  1:1428195/1‑140 (MQ=255)                     aaaaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATtca                                                                                                                 <  1:915225/140‑1 (MQ=255)                      aaaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATtcat                                                                                                                <  1:354992/140‑1 (MQ=255)                      aaaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATtcat                                                                                                                <  1:912619/140‑1 (MQ=255)                      aaaCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATtcat                                                                                                                <  2:897207/140‑1 (MQ=255)                            ttATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCAtcagttc                                                                                                          >  2:1028756/1‑140 (MQ=255)                            ttATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCAtcagttc                                                                                                          >  1:547784/1‑140 (MQ=255)                               tCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCAtcagttcagt                                                                                                       <  2:518856/140‑1 (MQ=255)                               tCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCAtcagttcagt                                                                                                       <  2:853402/140‑1 (MQ=255)                                         tttCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGaa                                                                                             >  2:710907/1‑140 (MQ=255)                                           tCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAAcc                                                                                           <  1:137301/140‑1 (MQ=255)                                              gTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                                                                                        <  2:1428195/140‑1 (MQ=255)                                                    tAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                                                                                        >  1:1038002/1‑134 (MQ=255)                                                    tAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGAc                                                                                        <  2:1038002/134‑1 (MQ=255)                                                    tAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                                                                                  >  2:1082758/1‑140 (MQ=255)                                                    tAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                                                                                  >  2:745518/1‑140 (MQ=255)                                                    tAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTcc                                                                                  >  2:7336/1‑140 (MQ=255)                                                      aGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCgg                                                                                >  1:1289305/1‑140 (MQ=255)                                                        ccATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa                                                                              <  2:1289305/140‑1 (MQ=255)                                                        ccATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCa                                                                              <  1:1082758/140‑1 (MQ=255)                                                           tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg                                                                           >  1:375133/1‑140 (MQ=255)                                                           tCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAg                                                                           >  1:1391130/1‑140 (MQ=255)                                                            cccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGc                                                                          >  2:451773/1‑140 (MQ=255)                                                             ccTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGcc                                                                         >  1:1204696/1‑140 (MQ=255)                                                                  tttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAata                                                                    >  1:922390/1‑140 (MQ=255)                                                                   ttCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAatat                                                                   >  2:301977/1‑140 (MQ=255)                                                                    tCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATAtt                                                                  >  1:1327593/1‑140 (MQ=255)                                                                       cGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGcc                                                                         <  1:939266/130‑1 (MQ=255)                                                                       cGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGcc                                                                         >  2:939266/1‑130 (MQ=255)                                                                         gCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCGGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTcc                                                             >  2:1212144/1‑140 (MQ=255)                                                                         gCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTcc                                                             >  2:863153/1‑140 (MQ=255)                                                                               aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCa                                                       <  2:547784/140‑1 (MQ=255)                                                                               aGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCa                                                       <  1:745518/140‑1 (MQ=255)                                                                                ggggTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAg                                                      <  2:516270/140‑1 (MQ=255)                                                                                   gTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   <  2:1391130/140‑1 (MQ=255)                                                                                   gTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   <  2:967115/140‑1 (MQ=255)                                                                                   gTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   <  1:301977/140‑1 (MQ=255)                                                                                   gTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   <  1:938357/140‑1 (MQ=255)                                                                                    tGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGc                                                  >  1:1311635/1‑140 (MQ=255)                                                                                      aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   <  2:1303282/137‑1 (MQ=255)                                                                                      aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   >  1:1303282/1‑137 (MQ=255)                                                                                      aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTa                                                >  1:853678/1‑140 (MQ=255)                                                                                       aCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTaa                                               <  2:1327593/140‑1 (MQ=255)                                                                                          tGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATg                                            >  2:1129353/1‑140 (MQ=255)                                                                                            gagagaTATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATgag                                          <  2:375133/140‑1 (MQ=255)                                                                                             agagaTATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATgaga                                         >  2:1299654/1‑140 (MQ=255)                                                                                               agaTATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGata                                       <  1:1028756/140‑1 (MQ=255)                                                                                                gaTATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTGCGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGatat                                      >  2:1295238/1‑140 (MQ=255)                                                                                                gaTATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGatat                                      <  2:1204696/140‑1 (MQ=255)                                                                                                 atatCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   <  2:249251/126‑1 (MQ=255)                                                                                                 atatCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACg                                                   >  1:249251/1‑126 (MQ=255)                                                                                                    tCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCg                                  >  1:576983/1‑140 (MQ=255)                                                                                                         gCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTaa                                               <  1:677331/122‑1 (MQ=255)                                                                                                         gCCATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTaa                                               >  2:677331/1‑122 (MQ=255)                                                                                                           cATTAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCttt                           >  2:70518/1‑140 (MQ=255)                                                                                                             ttAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCttttt                         <  2:243983/140‑1 (MQ=255)                                                                                                              tAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCtttttt                        >  1:818441/1‑140 (MQ=255)                                                                                                              tAAGACGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCtttttt                        <  2:797595/140‑1 (MQ=255)                                                                                                                  aCGGATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGt                    <  2:922390/140‑1 (MQ=255)                                                                                                                    ggATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCa                  <  1:7336/140‑1 (MQ=255)                                                                                                                    ggATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCa                  <  1:281277/140‑1 (MQ=255)                                                                                                                    ggATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCa                  <  2:120333/140‑1 (MQ=255)                                                                                                                     gATTTCTCCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCAc                 <  2:576983/140‑1 (MQ=255)                                                                                                                           tcCGCCAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTgg           <  1:1295238/140‑1 (MQ=255)                                                                                                                                cAGCGCGCTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACg      >  2:269293/1‑140 (MQ=255)                                                                                                                                    gcgcTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTcc  <  1:1129353/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCC  >  NC_000913/4309905‑4310175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑