BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,561,411	G→A	G161D (GGC→GAC)	kptA →	RNA 2'‑phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,561,411	0	G	A	100.0%	370.4 / NA	110	G161D (GGC→GAC)	kptA	RNA 2'‑phosphotransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (49/61); total (49/61)

Only 100 of 112 total aligned reads displayed.

CAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGC  >  NC_000913/4561281‑4561537

cAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCttt                                                                                                                       >  2:914295/1‑140 (MQ=255)

      cTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCa                                                                                                                 <  2:877887/140‑1 (MQ=255)

        gATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAg                                                                                                               <  1:619202/140‑1 (MQ=255)

          ttGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCg                                                                                                             >  1:55387/1‑140 (MQ=255)

          ttGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCg                                                                                                             <  2:932077/140‑1 (MQ=255)

                 tGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACg                                                                                                      <  1:368042/140‑1 (MQ=255)

                   aGCGTCACTACGTACATCTCTCTGCTGATGAGGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTAGCAAGCGGAAAACggg                                                                                                    >  2:257008/1‑140 (MQ=255)

                   aGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACggg                                                                                                    >  2:208062/1‑140 (MQ=255)

                   aGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACggg                                                                                                    >  2:1343646/1‑140 (MQ=255)

                   aGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACggg                                                                                                    >  1:129814/1‑140 (MQ=255)

                         aCTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTgg                                                                                              <  1:1047382/140‑1 (MQ=255)

                                  aTCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCa                                                                                     >  2:206934/1‑140 (MQ=255)

                                  aTCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCa                                                                                     >  1:213811/1‑140 (MQ=255)

                                  aTCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCa                                                                                     >  2:629480/1‑140 (MQ=255)

                                    ctctctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAc                                                                                   >  1:612589/1‑140 (MQ=255)

                                    ctctctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAc                                                                                   <  2:1477972/140‑1 (MQ=255)

                                     tctctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcg                                                                                  >  2:62495/1‑140 (MQ=255)

                                      ctctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcgg                                                                                 >  1:1588477/1‑140 (MQ=255)

                                       tctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACGGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggt                                                                                >  2:1393573/1‑140 (MQ=255)

                                       tctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggt                                                                                >  2:1304601/1‑140 (MQ=255)

                                       tctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggt                                                                                >  2:1017592/1‑140 (MQ=255)

                                       tctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggt                                                                                <  1:1443862/140‑1 (MQ=255)

                                       tctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggt                                                                                >  1:1213268/1‑140 (MQ=255)

                                       tctGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggt                                                                                >  2:1298010/1‑140 (MQ=255)

                                         tgctgATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttg                                                                              >  1:921339/1‑140 (MQ=255)

                                           ctgATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcg                                                                            >  1:1124382/1‑140 (MQ=255)

                                            tgatgaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcgg                                                                           >  1:1460142/1‑140 (MQ=255)

                                             gatgaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcggt                                                                          <  2:612589/140‑1 (MQ=255)

                                             gatgaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcggt                                                                          <  2:1077469/140‑1 (MQ=255)

                                             gatgaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcggt                                                                          <  1:1017592/140‑1 (MQ=255)

                                              atgaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcggtt                                                                         >  2:731452/1‑140 (MQ=255)

                                              atgaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcggtt                                                                         >  1:980426/1‑140 (MQ=255)

                                                gaAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGa                                                                       >  1:1074802/1‑140 (MQ=255)

                                                 aaGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAg                                                                      >  2:1507586/1‑140 (MQ=255)

                                                  aGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACGGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGt                                                                     <  1:1393573/140‑1 (MQ=255)

                                                     aaCGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTc                                                                  >  2:336698/1‑140 (MQ=255)

                                                     aaCGGCACGCAAAGAGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTc                                                                  >  2:253017/1‑140 (MQ=255)

                                                        ggCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcggt                                                                          <  1:1318651/129‑1 (MQ=255)

                                                        ggCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAcggttgcggt                                                                          >  2:1318651/1‑129 (MQ=255)

                                                        ggCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTg                                                               >  2:1323472/1‑140 (MQ=255)

                                                        ggCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTATTg                                                               <  2:407769/140‑1 (MQ=255)

                                                         gCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGa                                                              <  2:342757/140‑1 (MQ=255)

                                                          cACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAg                                                             <  1:1304601/140‑1 (MQ=255)

                                                           aCGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGt                                                            >  1:1541257/1‑140 (MQ=255)

                                                             gCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTgg                                                          >  1:1448476/1‑140 (MQ=255)

                                                                 aGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAg                                                      <  2:819530/140‑1 (MQ=255)

                                                                 aGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAg                                                      <  1:1298010/140‑1 (MQ=255)

                                                                  gTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAgg                                                     <  2:1124382/140‑1 (MQ=255)

                                                                   tGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAggg                                                    >  1:341588/1‑140 (MQ=255)

                                                                   tGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATAGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAggg                                                    >  1:319699/1‑140 (MQ=255)

                                                                     ggAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTg                                                               >  2:226600/1‑127 (MQ=255)

                                                                     ggAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTg                                                               <  1:226600/127‑1 (MQ=255)

                                                                         cAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACtt                                              >  2:375147/1‑140 (MQ=255)

                                                                         cAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACtt                                              <  1:1507586/140‑1 (MQ=255)

                                                                             acacGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCt                                          >  1:1336617/1‑140 (MQ=255)

                                                                                   aTCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGaa                                    <  1:253017/140‑1 (MQ=255)

                                                                                   aTCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGaa                                    <  2:1541257/140‑1 (MQ=255)

                                                                                    tCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAg                                   <  1:1042448/140‑1 (MQ=255)

                                                                                    tCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAg                                   <  2:678148/140‑1 (MQ=255)

                                                                                      gCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTg                                 >  2:1029734/1‑140 (MQ=255)

                                                                                      gCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAAAGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTg                                 >  2:1452616/1‑140 (MQ=255)

                                                                                       ccGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGa                                <  2:1075445/140‑1 (MQ=255)

                                                                                        cGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGAc                               <  1:914295/140‑1 (MQ=255)

                                                                                        cGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGAc                               <  1:683355/140‑1 (MQ=255)

                                                                                        cGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGAc                               <  1:669006/140‑1 (MQ=255)

                                                                                        cGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGAc                               <  1:629480/140‑1 (MQ=255)

                                                                                           ttATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTAACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCAc                            >  2:378595/1‑140 (MQ=255)

                                                                                             aTTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACtt                          >  2:556774/1‑140 (MQ=255)

                                                                                                  aaCCGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGc                     <  1:731452/140‑1 (MQ=255)

                                                                                                    ccGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCtt                   >  2:711796/1‑140 (MQ=255)

                                                                                                     cGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTa                  <  2:1074802/140‑1 (MQ=255)

                                                                                                     cGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTa                  <  1:324395/140‑1 (MQ=255)

                                                                                                     cGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTa                  <  2:505931/140‑1 (MQ=255)

                                                                                                     cGTCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTa                  <  1:62495/140‑1 (MQ=255)

                                                                                                       tCAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTaaa                >  1:1052918/1‑140 (MQ=255)

                                                                                                        cAAAGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAaat               >  1:1132050/1‑140 (MQ=255)

                                                                                                         aaaGCACAGGAAATGGCGAAACGAGACCTCCCCTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAaata              >  1:579873/1‑140 (MQ=255)

                                                                                                          aaGCACAGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAaataa             <  2:1213268/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAGTTGCa        <  1:413833/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:744031/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:476027/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:1029734/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:1619222/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:1588477/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:1556227/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:1460142/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:1171055/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:1132050/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:1323472/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:1343646/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:429200/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:375147/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:336698/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:206934/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  1:1452616/140‑1 (MQ=255)

                                                                                                               caGGAAATGGCGAAACGAGACCTCCCCTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCa        <  2:579873/140‑1 (MQ=255)

                                                                                                                 ggAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACt      <  1:556774/140‑1 (MQ=255)

                                                                                                                  gAAATGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACtg     >  1:867676/1‑140 (MQ=255)

                                                                                                                    aaTGGCGAAACGAGACCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACtgtg   <  2:980426/140‑1 (MQ=255)

                                                                                                                     aTGGCGAAACGAGAGCTCCCTTTCTTGCAGGCGGAAAAGGGGGTCTGGCTGACATCAACGCCTGCGGTTGCGTTTCTTGAGTGGTAAGGGCAACTTCCCTCATGAAGCGACCACTTGTAGCTTAAATAATTCCACTGTGc  >  2:273757/1‑140 (MQ=255)

CAGGGGCTGATTGCAGGTGAGCGTCACTACGTACATCTCTCTGCTGATGAAGCAACGGCACGCAAAGTGGGAGCAAGACACGGATCGCCGGTTATTTTAACCGTCAAAGCACAGGAAATGGCGAAACGAGGCCTCCCTTTCTGGCAAGCGGAAAACGGGGTCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGC  >  NC_000913/4561281‑4561537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`