Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,331	T→C	V200A (GTA→GCA)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,331	0	T	C	100.0%	234.0 / NA	66	V200A (GTA→GCA)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/32);  total (34/32)

GCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGAC  >  NC_000913/4631194‑4631445                                                                                                                                          |                                                                                                                   gCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCaa                                                                                                                  >  1:406358/1‑140 (MQ=255)     tgGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGt                                                                                                              <  1:204893/140‑1 (MQ=255)       gCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGc                                                                                                            >  1:780633/1‑140 (MQ=255)       gCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGc                                                                                                            >  1:1236474/1‑140 (MQ=255)          aaCCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTgg                                                                                                         <  2:406358/140‑1 (MQ=255)            ccGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACGCAGGCCTGGCAACAGTCCTggca                                                                                                       <  2:559260/140‑1 (MQ=255)               gCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGTTggcaggg                                                                                                    >  1:1186895/1‑140 (MQ=255)                  agagCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTggcagggcag                                                                                                 >  2:1552046/1‑140 (MQ=255)                      ccagccTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGAtgc                                                                                             <  1:258155/140‑1 (MQ=255)                      ccagccTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGAtgc                                                                                             <  2:1305779/140‑1 (MQ=255)                           ccaaCGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGAtgcctgcc                                                                                        <  2:1100773/138‑1 (MQ=255)                           cTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGAtgcctgcc                                                                                        <  1:1189350/140‑1 (MQ=255)                              aCGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGAt                                                                                     >  2:1493257/1‑140 (MQ=255)                              aCGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGAt                                                                                     <  2:1484090/140‑1 (MQ=255)                               cGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGAtt                                                                                    >  2:1329095/1‑140 (MQ=255)                                   tgtgACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCa                                                                                >  1:1437514/1‑140 (MQ=255)                                   tgtgACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCa                                                                                >  2:1375431/1‑140 (MQ=255)                                    gtgACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAg                                                                               >  2:1144514/1‑140 (MQ=255)                                    gtgACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAg                                                                               >  1:795324/1‑140 (MQ=255)                                           gTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCg                                                                        <  1:1180334/140‑1 (MQ=255)                                               tttAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTc                                                                    >  1:1446094/1‑140 (MQ=255)                                               tttAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTc                                                                    >  2:83924/1‑140 (MQ=255)                                                  aGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGc                                                                 <  2:1236474/140‑1 (MQ=255)                                                  aGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGc                                                                 <  1:1144514/140‑1 (MQ=255)                                                   gCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCa                                                                >  1:556010/1‑140 (MQ=255)                                                   gCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCa                                                                <  1:1375431/140‑1 (MQ=255)                                                     gTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAAt                                                              <  1:1275460/140‑1 (MQ=255)                                                      tCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATc                                                             >  1:629713/1‑140 (MQ=255)                                                      tCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATc                                                             >  2:567593/1‑140 (MQ=255)                                                      tCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATc                                                             >  1:160678/1‑140 (MQ=255)                                                         ggCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGGCTATCGCCTCGGCAATCAtt                                                          >  1:134558/1‑140 (MQ=255)                                                          gcgtgcgtCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCAttt                                                         <  2:1446094/140‑1 (MQ=255)                                                               cgtCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTg                                                    <  1:83924/140‑1 (MQ=255)                                                                     gTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGTTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGaac                                              <  2:1186895/140‑1 (MQ=255)                                                                     gTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTTCCAGACTATCGCCTCGGCAATCATTTCACTGGCGaac                                              <  2:1437514/140‑1 (MQ=255)                                                                     gTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGaac                                              <  1:567593/140‑1 (MQ=255)                                                                     gTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGaac                                              <  2:795324/140‑1 (MQ=255)                                                                       aaaCAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGaacaa                                            <  1:314573/140‑1 (MQ=255)                                                                       aaaCAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGaacaa                                            <  2:229886/140‑1 (MQ=255)                                                                        aaCAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGaacaac                                           >  1:1107022/1‑140 (MQ=255)                                                                          cAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAAccc                                         <  2:780633/140‑1 (MQ=255)                                                                               tCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    <  2:1113722/140‑1 (MQ=255)                                                                               tCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    <  2:66616/140‑1 (MQ=255)                                                                               tCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    >  2:786888/1‑140 (MQ=255)                                                                               tCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    <  2:268187/140‑1 (MQ=255)                                                                                ccGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    <  1:937377/139‑1 (MQ=255)                                                                                ccGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    >  2:937377/1‑139 (MQ=255)                                                                                   cTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGt                                >  1:877463/1‑140 (MQ=255)                                                                                    tGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTa                               >  2:1504112/1‑140 (MQ=255)                                                                                     ggCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTAc                              >  2:436594/1‑140 (MQ=255)                                                                                     ggCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTAc                              >  1:652958/1‑140 (MQ=255)                                                                                      gCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACt                             >  2:1200099/1‑140 (MQ=255)                                                                                           tttAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    <  2:891665/128‑1 (MQ=255)                                                                                           tttAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATa                                    >  1:891665/1‑128 (MQ=255)                                                                                              agagCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTcg                     <  1:1552046/140‑1 (MQ=255)                                                                                                agCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTcgcg                   <  2:1107022/140‑1 (MQ=255)                                                                                                agCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTcgcg                   >  2:940610/1‑140 (MQ=255)                                                                                                 gCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTcgcgc                  <  1:1504112/140‑1 (MQ=255)                                                                                                  cGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTcgcgcg                 <  1:1493257/140‑1 (MQ=255)                                                                                                    tATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTa               >  1:1055995/1‑140 (MQ=255)                                                                                                    tATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTa               >  1:82755/1‑140 (MQ=255)                                                                                                    tATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTa               >  1:1159096/1‑140 (MQ=255)                                                                                                     aTCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTAc              >  1:88756/1‑140 (MQ=255)                                                                                                     aTCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTAc              >  1:784822/1‑140 (MQ=255)                                                                                                                 aTGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGcgcgtc  <  2:652958/140‑3 (MQ=255)                                                                                                                 aTGAAAGCGGGTAACACAGGCCTGGCAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGAc  >  1:483662/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                   GCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGAC  >  NC_000913/4631194‑4631445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑