Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	520,655	G→A	K80K (AAG→AAA)	ybbP →	putative ABC transporter membrane subunit YbbP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	520,655	0	G	A	100.0%	215.6 / NA	61	K80K (AAG→AAA)	ybbP	putative ABC transporter membrane subunit YbbP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/37);  total (24/37)

CGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCTGAAACCGCAGGCGGGCAGCG  >  NC_000913/520523‑520791                                                                                                                                     |                                                                                                                                         cGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGa                                                                                                                                   >  2:499060/1‑140 (MQ=255)     cGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCCGATCACGCGAAGGTCCGCAAGCGTGGGTGGAGGAAACGCAAAACAGCGGCCTGAAAGACGGCAAACAACTGACttt                                                                                                                               >  2:20871/1‑140 (MQ=255)     cGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACttt                                                                                                                               <  1:1214990/140‑1 (MQ=255)     cGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGACAAACAGCTGACttt                                                                                                                               >  1:124324/1‑140 (MQ=255)     cGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGAGCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGGGGCTGGAGGAAGCGCAAAAGCGCGGCCCGAAAGTCGGGAAACAACTGACttt                                                                                                                               >  2:1470045/1‑140 (MQ=255)      gCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGGGGCTGGAGGAAACGCAAAAACGCGGCCTGAAAGTCGGCAAAAAGCTGACtttt                                                                                                                              >  2:284606/1‑139 (MQ=255)            agaAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCTAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCcaca                                                                                                                        <  2:1447696/140‑1 (MQ=255)            agaAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCcaca                                                                                                                        <  2:1522362/140‑1 (MQ=255)                gggCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGa                                                                                                                    >  2:1581837/1‑140 (MQ=255)                gggCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGa                                                                                                                    >  2:948783/1‑140 (MQ=255)                gggCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAACGCAAAAACGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGa                                                                                                                    >  2:732213/1‑140 (MQ=255)                  gCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGGGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGAcc                                                                                                                  >  1:744360/1‑140 (MQ=255)                  gCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGAcc                                                                                                                  >  1:501416/1‑140 (MQ=255)                  gCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGAcc                                                                                                                  >  1:872007/1‑140 (MQ=255)                     tAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCttt                                                                                                               <  1:414571/140‑1 (MQ=255)                     tAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCttt                                                                                                               <  1:1470045/140‑1 (MQ=255)                      aaGCCAGCAAAGCCGTGAGTTTATGGCGGGCTGTCGGGCGTTGCGCACCTCCCGCGAAATTCCCCCACCGTGGCTGGTGGAAGGGCTAAAGAGCGGCCTGCAAGTCTGCCAAAAGCTGACCTTCGCCACAAAGACCcttt                                                                                                              >  2:1072245/1‑139 (MQ=255)                      aaGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTg                                                                                                              >  2:1377174/1‑140 (MQ=255)                          cAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCACTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGTAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGTAgg                                                                                                          <  1:1072245/140‑1 (MQ=255)                                  ccGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGacacac                                                                                                  >  2:1421851/1‑140 (MQ=255)                                            aTGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGc                                                                                        >  1:707473/1‑140 (MQ=255)                                                 gggcgATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACg                                                                                   <  1:20871/140‑1 (MQ=255)                                                  ggcgATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGt                                                                                  >  1:42667/1‑140 (MQ=255)                                                   gcgATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTc                                                                                 >  1:1441866/1‑140 (MQ=255)                                                   gcgATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTc                                                                                 >  2:1388205/1‑140 (MQ=255)                                                    cgATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGACAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCa                                                                                <  2:124324/140‑1 (MQ=255)                                                       tCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAg                                                                             <  2:872007/140‑1 (MQ=255)                                                       tCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAg                                                                             <  1:489927/140‑1 (MQ=255)                                                        cGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGc                                                                            <  2:1149576/140‑1 (MQ=255)                                                          ggCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAGGCgg                                                                          <  1:973318/140‑1 (MQ=255)                                                          ggCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCgg                                                                          <  1:589286/140‑1 (MQ=255)                                                          ggCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCgg                                                                          <  1:1292139/140‑1 (MQ=255)                                                           gCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGt                                                                         <  1:1581837/140‑1 (MQ=255)                                                           gCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGt                                                                         <  1:1377174/140‑1 (MQ=255)                                                              ttGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGga                                                                      <  1:1175471/140‑1 (MQ=255)                                                              ttGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGga                                                                      <  2:370691/140‑1 (MQ=255)                                                              ttGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGga                                                                      <  1:219599/140‑1 (MQ=255)                                                               tGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAg                                                                             >  2:1624598/1‑132 (MQ=255)                                                               tGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAg                                                                             <  1:1624598/132‑1 (MQ=255)                                                                 cgcAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgatga                                                                   >  1:1385634/1‑140 (MQ=255)                                                                 cgcAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgatga                                                                   >  2:787662/1‑140 (MQ=255)                                                                  gcAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgatgat                                                                  <  1:19529/140‑1 (MQ=255)                                                                   cAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGata                                                                 <  1:1388205/140‑1 (MQ=255)                                                                    aGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGatat                                                                <  1:284606/140‑1 (MQ=255)                                                                    aGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGatat                                                                <  2:1441866/140‑1 (MQ=255)                                                                     gTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGga                                                                      >  1:68040/1‑133 (MQ=255)                                                                     gTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGga                                                                      <  2:68040/133‑1 (MQ=255)                                                                         aCGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGTCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAcc                                                           <  2:501416/140‑1 (MQ=255)                                                                         aCGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAcc                                                           <  1:417689/140‑1 (MQ=255)                                                                           gcgAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCg                                                                           <  2:987970/122‑1 (MQ=255)                                                                           gcgAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCg                                                                           >  1:987970/1‑122 (MQ=255)                                                                                gTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGGGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTa                                                    >  2:1007113/1‑140 (MQ=255)                                                                                gTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTa                                                    <  1:1421851/140‑1 (MQ=255)                                                                                gTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTa                                                    >  1:837701/1‑140 (MQ=255)                                                                                   ccGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATgg                                                 >  1:1598674/1‑140 (MQ=255)                                                                                    cGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGc                                                <  1:652347/140‑1 (MQ=255)                                                                                     gCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAAGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCg                                               >  1:907789/1‑140 (MQ=255)                                                                                      cAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGa                                              >  1:1543088/1‑140 (MQ=255)                                                                                            tGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCa                                        <  2:384487/140‑1 (MQ=255)                                                                                             ggCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCaa                                       <  2:837701/140‑1 (MQ=255)                                                                                                  gaggaAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTaa                                  <  2:707473/140‑1 (MQ=255)                                                                                                  gaggaAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTaa                                  <  1:499060/140‑1 (MQ=255)                                                                                                       aGCGCAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGA‑CTGCAAACTAATccccc                            >  1:920683/1‑140 (MQ=255)                                                                                                        gcgcAAAAGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATccccc                            <  1:948783/140‑1 (MQ=255)                                                                                                             aaaGCGCGGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGcc                       >  1:223281/1‑140 (MQ=255)                                                                                                                   cgGCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCTGAAAc                 >  2:140684/1‑140 (MQ=255)                                                                                                                     gCCTGAAAGTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCA‑GCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCTGAAACCGc              >  2:1163294/1‑140 (MQ=255)                                                                                                                             gTCGGCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCTGAAACCGCAGGCggg       <  2:744360/140‑1 (MQ=255)                                                                                                                                ggCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCTGAAACCGCAGGCGGGCAg    >  2:623845/1‑140 (MQ=255)                                                                                                                                 gCAAACAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGA‑CTGCAAACTAATCCCCCTGGCCTGAAACCGCAGGCGGGCAGCg  <  2:920683/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCTGAAACCGCAGGCGGGCAGCG  >  NC_000913/520523‑520791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑