Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 96,560 A→G T187A (ACT→GCT)  mraY → phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091396,5600AG100.0% 384.9 / NA 106T187A (ACT→GCT) mraYphospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (61/45);  total (61/45)

Only 100 of 106 total aligned reads displayed.

CGCTGGGTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCG  >  NC_000913/96423‑96694
                                                                                                                                         |                                                                                                                                      
cgcTGGGTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCt                                                                                                                                      >  2:451590/1‑140 (MQ=255)
    gggTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCa                                                                                                                                  >  1:1172346/1‑140 (MQ=255)
     ggTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCaa                                                                                                                                 <  2:1542050/140‑1 (MQ=255)
     ggTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCaa                                                                                                                                 <  1:1119467/140‑1 (MQ=255)
     ggTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCaa                                                                                                                                 <  2:288240/140‑1 (MQ=255)
     ggTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCaa                                                                                                                                 <  1:142019/140‑1 (MQ=255)
     ggTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCaa                                                                                                                                 <  1:1245324/140‑1 (MQ=255)
         tCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAAcgcg                                                                                                                             >  1:245811/1‑140 (MQ=255)
          cGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCgg                                                                                                                            >  2:421488/1‑140 (MQ=255)
           gCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGC‑ACGCGGTa                                                                                                                          <  2:1172346/140‑1 (MQ=255)
            ccTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTa                                                                                                                          <  2:913107/140‑1 (MQ=255)
            ccTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTa                                                                                                                          >  2:404176/1‑140 (MQ=255)
                cGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAAcc                                                                                                                      >  2:139523/1‑140 (MQ=255)
                cGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAAcc                                                                                                                      >  2:1495162/1‑140 (MQ=255)
                      gTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGATAAACCTGACCg                                                                                                                <  2:361244/140‑1 (MQ=255)
                            tGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGtc                                                                                                          >  1:978113/1‑140 (MQ=255)
                             gCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGtct                                                                                                         >  2:1546001/1‑140 (MQ=255)
                             gCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGtct                                                                                                         >  1:1241285/1‑140 (MQ=255)
                              ccGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGtctc                                                                                                        <  1:171317/140‑1 (MQ=255)
                                 gCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGAc                                                                                                     >  2:1461885/1‑140 (MQ=255)
                                 gCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGAc                                                                                                     >  2:1214717/1‑140 (MQ=255)
                                      gACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCt                                                                                                >  1:855154/1‑140 (MQ=255)
                                        cacGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTgg                                                                                              <  2:1580270/140‑1 (MQ=255)
                                           gCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCaa                                                                                           >  2:384598/1‑140 (MQ=255)
                                                  aCGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGcc                                                                                    <  1:36748/140‑1 (MQ=255)
                                                   cGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCg                                                                                   <  1:384598/140‑1 (MQ=255)
                                                                  cATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGcc                                                                    >  1:1425606/1‑140 (MQ=255)
                                                                    ttCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCgg                                                                  >  1:1155705/1‑140 (MQ=255)
                                                                     tCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggt                                                                 >  2:647124/1‑140 (MQ=255)
                                                                     tCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggt                                                                 >  1:480900/1‑140 (MQ=255)
                                                                     tCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggt                                                                 >  2:1458325/1‑140 (MQ=255)
                                                                     tCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggt                                                                 >  1:8225/1‑140 (MQ=255)
                                                                     tCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggt                                                                 <  1:1617670/140‑1 (MQ=255)
                                                                     tCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggt                                                                 >  2:283567/1‑140 (MQ=255)
                                                                        ttAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggtggt                                                              >  2:1237242/1‑140 (MQ=255)
                                                                          aaaGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGttt                                                            <  1:421488/140‑1 (MQ=255)
                                                                             gATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTgc                                                         <  1:110359/140‑1 (MQ=255)
                                                                             gATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTgc                                                         >  1:1491402/1‑140 (MQ=255)
                                                                               tgtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTgcgc                                                       >  1:428072/1‑140 (MQ=255)
                                                                               tgtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTgcgc                                                       >  1:1567245/1‑140 (MQ=255)
                                                                                gtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCt                                                      >  1:6862/1‑140 (MQ=255)
                                                                                 tgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCtg                                                     >  2:1167882/1‑140 (MQ=255)
                                                                                 tgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCtg                                                     >  1:576926/1‑140 (MQ=255)
                                                                                 tgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCtg                                                     >  2:246877/1‑140 (MQ=255)
                                                                                  gATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCtgg                                                    <  1:1237242/140‑1 (MQ=255)
                                                                                     gCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCtggtgg                                                 <  1:669705/140‑1 (MQ=255)
                                                                                        gCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGt                                              >  1:724064/1‑140 (MQ=255)
                                                                                         cAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTgcg                                                        <  1:277789/129‑1 (MQ=255)
                                                                                         cAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTgcg                                                        >  2:277789/1‑129 (MQ=255)
                                                                                           gCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTggg                                           <  2:1001207/140‑1 (MQ=255)
                                                                                              ggggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGa                                        >  1:1208341/1‑140 (MQ=255)
                                                                                                ggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGAcc                                      >  1:799355/1‑140 (MQ=255)
                                                                                                 gCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCg                                     <  1:1546001/140‑1 (MQ=255)
                                                                                                    gTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCa                                  >  1:995321/1‑140 (MQ=255)
                                                                                                     ttCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCaa                                 >  1:1022799/1‑140 (MQ=255)
                                                                                                     ttCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCaa                                 >  1:261819/1‑140 (MQ=255)
                                                                                                     ttCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTATCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCaa                                 >  2:360881/1‑140 (MQ=255)
                                                                                                      tCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAat                                >  1:1056251/1‑140 (MQ=255)
                                                                                                      tCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAat                                >  1:513606/1‑140 (MQ=255)
                                                                                                         aCATTCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCAACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATg                             >  2:984277/1‑140 (MQ=255)
                                                                                                            ttCTGCTGGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAAc                          >  2:293917/1‑140 (MQ=255)
                                                                                                               tgctgGCTTACTTCGTCATTGTGGGTGCTGGCAGCGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACttt                       >  2:193497/1‑140 (MQ=255)
                                                                                                               tgctgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACttt                       >  2:1249543/1‑140 (MQ=255)
                                                                                                               tgctgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACttt                       >  2:1086441/1‑140 (MQ=255)
                                                                                                               tgctgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACttt                       >  1:155253/1‑140 (MQ=255)
                                                                                                               tgctgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACttt                       >  2:46973/1‑140 (MQ=255)
                                                                                                               tgctgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACttt                       >  2:660035/1‑140 (MQ=255)
                                                                                                                 ctgGCTTACTTCGTCATTGTGGGTGCTGGCAGCGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGc                     <  1:193497/140‑1 (MQ=255)
                                                                                                                 ctgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGc                     >  1:853714/1‑140 (MQ=255)
                                                                                                                  tgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGcc                    <  2:245811/140‑1 (MQ=255)
                                                                                                                  tgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGcc                    <  1:139523/140‑1 (MQ=255)
                                                                                                                  tgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGcc                    <  1:283567/140‑1 (MQ=255)
                                                                                                                  tgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGcc                    <  2:1022799/140‑1 (MQ=255)
                                                                                                                  tgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGcc                    <  1:439834/140‑1 (MQ=255)
                                                                                                                  tgGCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGcc                    <  1:647124/140‑1 (MQ=255)
                                                                                                                   ggCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCa                   <  2:1241285/140‑1 (MQ=255)
                                                                                                                    gCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAata                               <  2:1438296/127‑1 (MQ=255)
                                                                                                                    gCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAata                               >  1:1438296/1‑127 (MQ=255)
                                                                                                                    gCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTg                      >  1:1275838/1‑136 (MQ=255)
                                                                                                                    gCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTg                      <  2:1275838/136‑1 (MQ=255)
                                                                                                                    gCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAg                  >  2:1557154/1‑140 (MQ=255)
                                                                                                                    gCTTACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAg                  >  2:274555/1‑140 (MQ=255)
                                                                                                                       tACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa               <  2:1088496/140‑1 (MQ=255)
                                                                                                                       tACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa               <  1:293917/140‑1 (MQ=255)
                                                                                                                       tACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa               <  1:1316488/140‑1 (MQ=255)
                                                                                                                       tACTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa               <  2:1491402/140‑1 (MQ=255)
                                                                                                                         cTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGGCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt             <  2:1056251/140‑1 (MQ=255)
                                                                                                                         cTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa               <  1:634708/138‑1 (MQ=255)
                                                                                                                         cTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa               >  2:634708/1‑138 (MQ=255)
                                                                                                                         cTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt             <  1:1458325/140‑1 (MQ=255)
                                                                                                                         cTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt             <  2:1208341/140‑1 (MQ=255)
                                                                                                                         cTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt             <  2:8225/140‑1 (MQ=255)
                                                                                                                         cTTCGTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt             <  2:1567245/140‑1 (MQ=255)
                                                                                                                             gTCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCa         <  1:1495162/140‑1 (MQ=255)
                                                                                                                              tCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCat        >  1:489356/1‑140 (MQ=255)
                                                                                                                              tCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCat        >  1:400991/1‑140 (MQ=255)
                                                                                                                              tCATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCat        >  1:1006541/1‑140 (MQ=255)
                                                                                                                               cATTGTGGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCata       <  2:428072/140‑1 (MQ=255)
                                                                                                                                    tgGGTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCg  >  2:367/1‑140 (MQ=255)
                                                                                                                                      ggTGCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCa         <  2:912897/131‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CGCTGGGTGTCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCG  >  NC_000913/96423‑96694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑