Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,010,898	C→T	A312V (GCC→GTC)	uup →	ATP‑binding protein with possible role in replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,010,898	0	C	T	100.0%	201.6 / NA	59	A312V (GCC→GTC)	uup	ATP‑binding protein with possible role in replication
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/26);  total (33/26)

AGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGG  >  NC_000913/1010763‑1011033                                                                                                                                        |                                                                                                                                        agGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGc                                                                                                                                     >  2:616295/1‑140 (MQ=255)      tgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTc                                                                                                                                <  2:246407/140‑1 (MQ=255)       ggATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTcc                                                                                                                               >  2:291370/1‑140 (MQ=255)        gATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCg                                                                                                                              >  2:563925/1‑140 (MQ=255)             gCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTaaa                                                                                                                         >  1:1175343/1‑140 (MQ=255)                     aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGtttt                                                                                                                 >  1:317854/1‑140 (MQ=255)                       cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCg                                                                                                               >  2:757687/1‑140 (MQ=255)                          aGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGaaa                                                                                                            <  2:317854/140‑1 (MQ=255)                          aGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGaaa                                                                                                            <  2:265051/140‑1 (MQ=255)                               gCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGaa                                                                                                       >  2:126778/1‑140 (MQ=255)                               gCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGaa                                                                                                       >  2:767077/1‑140 (MQ=255)                                ccGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAg                                                                                                      <  1:757687/140‑1 (MQ=255)                                 cGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGa                                                                                                     <  2:741549/140‑1 (MQ=255)                                 cGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGa                                                                                                     >  1:1085568/1‑140 (MQ=255)                                    aCCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGt                                                                                                  <  1:1031222/140‑1 (MQ=255)                                    aCCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGt                                                                                                  <  1:563925/140‑1 (MQ=255)                                         tAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCt                                                                                             >  2:893036/1‑140 (MQ=255)                                         tAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCt                                                                                             <  2:276558/140‑1 (MQ=255)                                           aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                           <  2:946386/140‑1 (MQ=255)                                                ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGa                                                                                                     >  2:1145426/1‑125 (MQ=255)                                                ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGa                                                                                                     <  1:1145426/125‑1 (MQ=255)                                                ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                           >  1:414171/1‑135 (MQ=255)                                                ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTAc                                                                                           <  2:414171/135‑1 (MQ=255)                                                ggCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGAGGAAGAGGTGAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGt                                                                                      >  2:1453390/1‑140 (MQ=255)                                                   cgcgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTaa                                                                                   >  2:785188/1‑140 (MQ=255)                                                     cgTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACg                                                                                 >  1:1623028/1‑140 (MQ=255)                                                           cgcCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGc                                                                           >  2:453606/1‑140 (MQ=255)                                                           cgcCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGc                                                                           >  1:1056020/1‑140 (MQ=255)                                                                        aTGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGa                                                              >  1:360197/1‑140 (MQ=255)                                                                        aTGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGa                                                              >  1:1217327/1‑140 (MQ=255)                                                                        aTGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGa                                                              >  2:1576737/1‑140 (MQ=255)                                                                          gCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGAtt                                                            >  1:667785/1‑140 (MQ=255)                                                                             tCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGAttttt                                                         <  2:360197/140‑1 (MQ=255)                                                                              cgcgAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTc                                                        <  1:1272993/140‑1 (MQ=255)                                                                                  aaCGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGcc                                                    <  2:1056020/140‑1 (MQ=255)                                                                                   aCGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGccc                                                   <  2:1217327/140‑1 (MQ=255)                                                                                       ggtgAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGt                                               >  1:835125/1‑140 (MQ=255)                                                                                             cGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTc                                                        >  2:455027/1‑125 (MQ=255)                                                                                             cGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTc                                                        <  1:455027/125‑1 (MQ=255)                                                                                              gTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGt                                        <  1:126778/140‑1 (MQ=255)                                                                                              gTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGt                                        <  1:453606/140‑1 (MQ=255)                                                                                                cgcgAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTgg                                      >  2:955731/1‑140 (MQ=255)                                                                                                   gAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGa                                   <  2:667785/140‑1 (MQ=255)                                                                                                          tGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGa                                   >  2:402540/1‑133 (MQ=255)                                                                                                          tGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGa                                   <  1:402540/133‑1 (MQ=255)                                                                                                          tGGGTACCGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAAtt                            >  2:841032/1‑140 (MQ=255)                                                                                                                 cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                     <  1:767077/140‑1 (MQ=255)                                                                                                                 cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                     >  1:147362/1‑140 (MQ=255)                                                                                                                 cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                     <  1:841032/140‑1 (MQ=255)                                                                                                                 cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                     >  2:16800/1‑140 (MQ=255)                                                                                                                 cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                     >  1:73547/1‑140 (MQ=255)                                                                                                                 cGCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGa                     >  2:1171643/1‑140 (MQ=255)                                                                                                                  gCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGAt                    <  1:616295/140‑1 (MQ=255)                                                                                                                  gCAAAGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGAt                    <  1:955731/140‑1 (MQ=255)                                                                                                                      aGATGCAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGt                >  1:978082/1‑140 (MQ=255)                                                                                                                           cAGGTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGaa           <  1:291370/140‑1 (MQ=255)                                                                                                                              gTGGAAGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATgg        >  2:1326703/1‑140 (MQ=255)                                                                                                                                  aaGAGGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGCTAACGGCAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGAGc    >  1:219794/1‑140 (MQ=255)                                                                                                                                    gagGTCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCgg  <  1:1171643/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTACGTGGCGACAAAATTGCCCTGATTGGTCCGAATGGGTGCGG  >  NC_000913/1010763‑1011033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑