Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,765	A→G	D394G (GAC→GGC)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,765	0	A	G	100.0%	179.2 / NA	50	D394G (GAC→GGC)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/26);  total (24/26)

AGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTC  >  NC_000913/1066632‑1066893                                                                                                                                      |                                                                                                                                 aGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGa                                                                                                                            <  2:594008/140‑1 (MQ=255)  gCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGAt                                                                                                                           <  2:341110/140‑1 (MQ=255)    aaaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCTCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATgg                                                                                                                         >  2:1039540/1‑140 (MQ=255)     aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGa                                                                                                                            <  2:234105/136‑1 (MQ=255)     aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGa                                                                                                                            >  1:234105/1‑136 (MQ=255)     aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGt                                                                                                                        <  2:427370/140‑1 (MQ=255)     aaGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGt                                                                                                                        <  2:1034042/140‑1 (MQ=255)            tCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGcc                                                                                                                 <  1:798586/140‑1 (MQ=255)             cgcgATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGccc                                                                                                                >  1:682228/1‑140 (MQ=255)              gcgATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCg                                                                                                               <  2:930015/140‑1 (MQ=255)              gcgATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCg                                                                                                               <  1:796580/140‑1 (MQ=255)                 aTTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATg                                                                                                            >  1:886117/1‑140 (MQ=255)                    tgatgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGAt                                                                                                         <  2:690869/140‑1 (MQ=255)                    tgatgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGAt                                                                                                         <  1:271675/140‑1 (MQ=255)                      atgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATaa                                                                                                       <  1:1526152/140‑1 (MQ=255)                         aaaaTTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGtt                                                                                                    <  1:1095581/140‑1 (MQ=255)                             ttGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGAt                                                                                                <  1:191936/140‑1 (MQ=255)                                     gtgtATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGtt                                                                                        <  2:682228/140‑1 (MQ=255)                                        tATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaa                                                                                     >  1:685813/1‑140 (MQ=255)                                          tCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaatg                                                                                   <  2:886117/140‑1 (MQ=255)                                             gagTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAg                                                                                >  1:927738/1‑140 (MQ=255)                                              agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCTCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGc                                                                               <  1:1039540/140‑1 (MQ=255)                                              agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATa                                                                                               >  1:279661/1‑124 (MQ=255)                                              agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATa                                                                                               <  2:279661/124‑1 (MQ=255)                                              agTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGc                                                                               >  1:867105/1‑140 (MQ=255)                                                tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  2:1478553/1‑140 (MQ=255)                                                tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  1:519123/1‑140 (MQ=255)                                                tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  1:658262/1‑140 (MQ=255)                                                tGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTGTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                             >  2:1511161/1‑140 (MQ=255)                                                  cGGAACAGTTACGTAATGCCGATGTGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTg                                                                           >  2:479247/1‑140 (MQ=255)                                                  cGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTg                                                                           >  1:545646/1‑140 (MQ=255)                                                     aaCAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGaaa                                                                        <  2:519123/140‑1 (MQ=255)                                                          ttACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACa                                                                   <  2:545646/140‑1 (MQ=255)                                                                     cGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcccc                                                        <  1:926017/140‑1 (MQ=255)                                                                           gTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACGGAAAACTCCCCCGCgag                                                  <  2:72945/140‑1 (MQ=255)                                                                            ttAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgaga                                                 >  2:1208075/1‑140 (MQ=255)                                                                            ttAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgaga                                                 >  2:118351/1‑140 (MQ=255)                                                                               aCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgcg                                                    <  1:718580/134‑1 (MQ=255)                                                                               aCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCcgcg                                                    >  2:718580/1‑134 (MQ=255)                                                                                      aGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                                  <  1:805152/129‑1 (MQ=255)                                                                                      aGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                                  >  2:805152/1‑129 (MQ=255)                                                                                         cACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGAGAGAAGCGGGGGAGTCg                                    <  2:685813/140‑1 (MQ=255)                                                                                            cTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                                 >  2:1391032/1‑140 (MQ=255)                                                                                               cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCg                                    >  1:1457809/1‑134 (MQ=255)                                                                                               cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCAGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCg                                    <  2:1457809/134‑1 (MQ=255)                                                                                                                      aGCGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTc       >  1:473917/1‑140 (MQ=255)                                                                                                                        cGGTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTg     <  1:1208075/140‑1 (MQ=255)                                                                                                                          gTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGtt   >  2:564524/1‑140 (MQ=255)                                                                                                                          gTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGtt   <  1:339/140‑1 (MQ=255)                                                                                                                          gTTGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGtt   >  2:339/1‑140 (MQ=255)                                                                                                                           ttGCCCGATAGGCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTc  >  1:83667/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                 AGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTC  >  NC_000913/1066632‑1066893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑