Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,141,920	A→G	R816R (CGT→CGC)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,141,920	0	A	G	100.0%	210.4 / NA	59	R816R (CGT→CGC)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)

TGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGA  >  NC_000913/1141783‑1142053                                                                                                                                          |                                                                                                                                      tGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTcgcg                                                                                                                                     >  2:616099/1‑140 (MQ=255)  gTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGa                                                                                                                                    >  2:413307/1‑140 (MQ=255)   ttCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGAt                                                                                                                                   <  1:1348262/140‑1 (MQ=255)    tCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAATGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATa                                                                                                                                  >  2:1024549/1‑140 (MQ=255)    tCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATa                                                                                                                                  <  1:616099/140‑1 (MQ=255)      gcgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGc                                                                                                                                <  1:905954/140‑1 (MQ=255)      gcgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGc                                                                                                                                <  2:1395346/140‑1 (MQ=255)      gcgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGc                                                                                                                                >  2:305964/1‑140 (MQ=255)       cgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGcg                                                                                                                               >  2:188397/1‑140 (MQ=255)               ttCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCgacgacgac                                                                                                                       <  1:305964/140‑1 (MQ=255)                tCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGacgacgacg                                                                                                                      <  2:420984/140‑1 (MQ=255)                tCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGacgacgacg                                                                                                                      <  2:202272/140‑1 (MQ=255)                tCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGacgacgacg                                                                                                                      <  1:1001015/140‑1 (MQ=255)                  aaCCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCt                                                                                                                    <  1:413307/140‑1 (MQ=255)                     cTGTACATCTAGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGAc                                                                                                                 <  1:1114283/140‑1 (MQ=255)                         aCATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACt                                                                                                             <  2:970442/140‑1 (MQ=255)                            tCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTAc                                                                                                          >  2:729281/1‑140 (MQ=255)                             cTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTAcg                                                                                                         >  1:1575470/1‑140 (MQ=255)                                gCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACgcgc                                                                                                      <  1:250639/140‑1 (MQ=255)                                  ggACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAg                                                                                                    >  2:1483330/1‑140 (MQ=255)                                   gACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAgg                                                                                                   >  2:730027/1‑140 (MQ=255)                                              ggATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGa                                                                                        >  1:1568214/1‑140 (MQ=255)                                                 tAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcg                                                                                     >  1:525982/1‑140 (MQ=255)                                                  aGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcgg                                                                                    >  2:51196/1‑140 (MQ=255)                                                    cGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcggcg                                                                                  >  2:511332/1‑140 (MQ=255)                                                    cGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcggcg                                                                                  >  2:1106181/1‑140 (MQ=255)                                                    cGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcggcg                                                                                  >  2:104954/1‑140 (MQ=255)                                                      gATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCgag                                                                                >  1:702559/1‑140 (MQ=255)                                                         ccAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAac                                                                             >  2:63450/1‑140 (MQ=255)                                                             aCTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAacgacg                                                                         <  1:697925/140‑1 (MQ=255)                                                               tttGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGAcgcg                                                                       <  1:1106181/140‑1 (MQ=255)                                                                       agGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCgg                                                                      >  1:18824/1‑133 (MQ=255)                                                                       agGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCgg                                                                      <  2:18824/133‑1 (MQ=255)                                                                                ccGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCa                                                      <  1:51196/140‑1 (MQ=255)                                                                                  ggAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACg                                                    <  2:525982/140‑1 (MQ=255)                                                                                   gagaCGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACgg                                                   >  1:852314/1‑140 (MQ=255)                                                                                       cgcgCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTAt                                               <  1:1483330/140‑1 (MQ=255)                                                                                       cgcgCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTAt                                               <  2:1575470/140‑1 (MQ=255)                                                                                        gcgcACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAc                                                              >  1:1192932/1‑124 (MQ=255)                                                                                        gcgcACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAc                                                              <  2:1192932/124‑1 (MQ=255)                                                                                        gcgcACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATc                                              >  2:364175/1‑140 (MQ=255)                                                                                            aCGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCa                                          <  1:364175/140‑1 (MQ=255)                                                                                            aCGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGATTATCAGCa                                          <  1:63450/140‑1 (MQ=255)                                                                                                 aCGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGtt                                     <  1:559441/140‑1 (MQ=255)                                                                                                  cGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTtc                                    <  1:849054/140‑1 (MQ=255)                                                                                                     tCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTctct                                 <  1:188397/140‑1 (MQ=255)                                                                                                     tCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTctct                                 <  1:80525/140‑1 (MQ=255)                                                                                                          ggCATTGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTg                            >  2:895665/1‑140 (MQ=255)                                                                                                               tGGCGACTGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGtt                       >  1:662110/1‑140 (MQ=255)                                                                                                                      tGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGc                >  2:1229428/1‑140 (MQ=255)                                                                                                                      tGGGTTGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGc                <  1:729281/140‑1 (MQ=255)                                                                                                                      tGGGTTGGATAACGATCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGc                <  1:1229428/140‑1 (MQ=255)                                                                                                                          ttGGATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGtt            <  1:511332/140‑1 (MQ=255)                                                                                                                             gATAACGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTgc         >  1:679873/1‑140 (MQ=255)                                                                                                                                  cGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTgg                   <  2:1243288/125‑1 (MQ=255)                                                                                                                                  cGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTgg                   >  1:1243288/1‑125 (MQ=255)                                                                                                                                  cGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTAc    >  2:1251806/1‑140 (MQ=255)                                                                                                                                  cGCTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTAc    >  1:750070/1‑140 (MQ=255)                                                                                                                                    cTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGtt            <  2:1190693/130‑1 (MQ=255)                                                                                                                                    cTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGtt            >  1:1190693/1‑130 (MQ=255)                                                                                                                                    cTCGTCGCGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGa  >  2:695239/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGA  >  NC_000913/1141783‑1142053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑